Use of X-chromosome inactivation pattern to determine the clonal origins of uterine leiomyoma and leiomyosarcoma

Zhang, Peng; Zhang, Cunxian; Hao, Jie; Sung, C. James; Quddus, M. Ruhul; Steinhoff, Margaret M.; Lawrence, W. Dwayne

doi:10.1016/j.humpath.2006.05.005

Cited by 58 publications

(38 citation statements)

References 16 publications

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“…Herein, the term overexpression refers to an expression exceeding that of the matching myometrium. Despite the monoclonal origin of UL (Townsend et al, 1970;Mashal et al, 1994;Hashimoto et al, 1995;Zhang et al, 2006) overexpression of HMGA2 must not necessarily be due to mutations affecting the gene itself. It has recently been described that HMGA2 is regulated by microRNAs of the let-7 family (Lee and Dutta, 2007;Mayr et al, 2007;Park et al, 2007;Shell et al, 2007;Kumar et al, 2008;Motoyama et al, 2008;Peng et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Overexpression of HMGA2 in uterine leiomyomas points to its general role for the pathogenesis of the disease

Klemke

Meyer

Nezhad

et al. 2008

Genes Chromosomes & Cancer

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An overexpression of HMGA2 is supposed to be a key event in the genesis of leiomyoma with chromosomal rearrangements affecting the region 12q14-15 targeting the HMGA2 gene, but gene expression data regarding differences between uterine leiomyomas with and those without 12q14-15 aberrations are insufficient. To address the question whether HMGA2 is only upregulated in the 12q14-15 subgroup, the expression of HMGA2 was analyzed in a comprehensive set of leiomyomas (n = 180) including tumors with 12q14-15 chromosomal aberrations (n = 13) and matching myometrial tissues (n = 51) by quantitative RT-PCR. The highest expression levels for HMGA2 were observed in tumors with rearrangements affecting the region 12q14-15, but although HMGA2 is expressed at lower levels in leiomyomas without such aberrations, the comparison between the expression in myomas and matching myometrial tissues indicates a general upregulation of HMGA2 regardless of the presence or absence of such chromosomal abnormalities. The significant (P < 0.05) overexpression of HMGA2 also in the group of fibroids without chromosomal aberrations of the 12q14-15 region suggests a general role of HMGA2 in the development of the disease.

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Section: Discussionmentioning

confidence: 99%

Overexpression of HMGA2 in uterine leiomyomas points to its general role for the pathogenesis of the disease

Klemke

Meyer

Nezhad

et al. 2008

Genes Chromosomes & Cancer

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“…Although many women are asymptomatic and thus unaware of their condition, others have aggravating symptoms, such as chronic pelvic pain, dysfunctional uterine bleeding and anemia, which require treatment. Usually, multiple polyps on the uterus of the same patient represent ULs where each polyp has an independent genetic origin (1). ULs are clinically apparent in approximately 25% of women of reproductive age, with data suggesting that more than 75% of women present at least one asymptomatic myoma during their lifetime.…”

Section: Introductionmentioning

confidence: 99%

SPARC-like1 mRNA is overexpressed in human uterine leiomyoma

Mencalha

Levinsphul²,

Deterling³

et al. 2008

Mol Med Report

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Abstract. Uterine leiomyomas (ULs), also known as fibroids, are benign and monoclonal tumors frequently found in the female population. The genetic alterations that contribute to UL tumor development have not been well established, and the goal of this study was to reveal gene expression variation between ULs and healthy uterine tissue. We compared the gene expression profiles of 13 UL tumors with that of their adjacent normal tissue using the Differential Display mRNA assay (DDRT-PCR). Among the genes upregulated in some of the UL samples, several genes previously described in the context of cancer were identified, namely LIMK1, MCM3 and UHRF1. In addition, we identified a cDNA present in UL samples from distinct patients, which was absent from their normal tissue. Direct sequencing of this cDNA revealed a human SPARC-like1 (SPARCL1) mRNA homology. Through semi-quantitative PCR, we demonstrated that SPARCL1 was upregulated in approximately 77% of UL samples, but was absent in normal tissue. Real-time PCR (QPCR) revealed that SPARCL1 expression was increased 5-fold in ULs compared to adjacent normal tissue. These results suggest that the SPARCL1 gene is involved in UL development.

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“…Leiomyoma have monoclonal origin, arise from benign cases or may develop de nova. 7 To consider a leiomyoma as malignant-the mitotic figures should be greater than 15/10 High Power Field, 8 Tumour cell necrosis, nuclear atypia and pleomorphism are noted.…”

Section: Discussionmentioning

confidence: 99%

A Huge Parasitic Fibroid Causing Hernia

Geetha¹,

Hameed²,

Seethalakshmi³

et al. 2014

jemds

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Leiomyoma is the most common estrogen dependent benign tumour of the uterus occurring in the reproductive age. It is composed of smooth muscle and fibrous tissue. Asymptomatic myomas can be present in 50% of cases. It causes mainly menstrual problems like menorrhagia, metorrhagia, dysmenorrhoea and also infertility. Nowadays it is very rare to get huge myomas because most of the patients for any vague, trivial complaints undergoes scan, which picks up even very small myomas. We are presenting a very huge fibroid causing hernia in a patient 48years old P3L3 who developed hernia, following puerperal sterilization done. She had a hernia repair done 20 years ago. Now she developed recurrent hernia due to the huge abdominal mass 22×22cms of a parasitic fibroid. She was taken up for laporotomy. The mass was removed in addition total hysterectomy with bilateral salpingo oophorectomy was done. This case is presented here because of its rarity and its management was quite tough-a Herculean task of course. KEYWORDS: Hernia, parasitic fibroid, Subserous fibroid, total abdominal hysterectomy with salpingo-oophorectomy. CASE REPORT:A 48years old lady P3L3 got admitted with the history of menorrhagia, abdominal pain and breathlessness since 6months. On examination she was found to be anemic. Uterus was palpable up to 24 weeks size and she had a separate huge mass 22×22cms size extending up to the umbilicus and the right lumbar region. She had a scar in the infra umbilical region with incisional hernia. Cervix was healthy.No significant medical history present. She was transfused with two units of blood. Other blood investigations were normal. X-Ray chest showed cardiomegaly with left ventricular hypertrophy. Intra venous pyelogram showed bilateral hydroureter. Ultrasound showed umbilical hernia, uterus had multiple fibroids-sizes: 20×17×10cms and a huge fundal fibroid of size 22×22cms. The right ovary was cystic. Computed tomography showed large heterogeneous multi loculated abdomino pelvic mass of size 22×20cms with internal degeneration and calcifications from the pelvis. MANAGEMENT:Patient was taken up for laporotomy. There were plenty of adhesions which were released. A huge subserous fundal fibroid, 24×24cms with a twisted pedicle almost getting detached was attached to the Fundus of the uterus on the right side. There were plenty of vessels from the omentum feeding the fibroid. The whole omentum was attached to the surface of the fibroid. It was very vascular. The clamps were applied on the twisted pedicle of the subserous fibroid as well as on the huge vessels which were feeding it.Thanks to the general surgeon, the task was made easy. Part of the omentum had to be sacrificed because it was adherent to the surface of the subserous fibroid. The uterus was enlarged to 24 weeks size which had intramural fibroids. Total abdominal hysterectomy with bilateral salpingo oophorectomy was done. Hernia repair was done meticulously with mesh.

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Use of X-chromosome inactivation pattern to determine the clonal origins of uterine leiomyoma and leiomyosarcoma

Cited by 58 publications

References 16 publications

Overexpression of HMGA2 in uterine leiomyomas points to its general role for the pathogenesis of the disease

Overexpression of HMGA2 in uterine leiomyomas points to its general role for the pathogenesis of the disease

SPARC-like1 mRNA is overexpressed in human uterine leiomyoma

A Huge Parasitic Fibroid Causing Hernia

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