Use of the Atomic Force Microscope To Determine the Effect of Substratum Surface Topography on Bacterial Adhesion

Boyd, Robert; Verran, Joanna; Jones, Martin V.; Bhakoo, Manmohan

doi:10.1021/la011142p

Cited by 135 publications

(108 citation statements)

References 18 publications

Supporting

Mentioning

103

Contrasting

Order By: Relevance

“…There is a positive correlation between protein adsorption and short-term bacterial attachment with surface property changes (Cunliffe et al 2003). Also, the strength of bacterial attachment has been positively correlated to surface topography (Boyd et al 2002). Detachment of bacteria is affected by surface properties (Powell and Slater 1982) and bacteria are most readily removed from the smoothest, most hydrophilic, neutral surfaces (Pasmore et al 2002).…”

Section: Introductionmentioning

confidence: 88%

Influence of nanophase titania topography on bacterial attachment and metabolism

Webster¹

2008

IJN

View full text Add to dashboard Cite

Surfaces with nanophase compared to conventional (or nanometer smooth) topographies are known to have different properties of area, charge, and reactivity. Previously published research indicates that the attachment of certain bacteria (such as Pseudomonas fl uorescens 5RL) is higher on surfaces with nanophase compared to conventional topographies, however, their effect on bacterial metabolism is unclear. Results presented here show that the adhesion of Pseudomonas fl uorescens 5RL and Pseudomonas putida TVA8 was higher on nanophase than conventional titania. Importantly, in terms of metabolism, bacteria attached to the nanophase surfaces had higher bioluminescence rates than on the conventional surfaces under all nutrient conditions. Thus, the results from this study show greater select bacterial metabolism on nanometer than conventional topographies, critical results with strong consequences for the design of improved biosensors for bacteria detection.

show abstract

Section: Introductionmentioning

confidence: 88%

Influence of nanophase titania topography on bacterial attachment and metabolism

Webster¹

2008

IJN

View full text Add to dashboard Cite

show abstract

“…Boyd et al (8) found that enhanced adhesion of S. aureus occurred on rougher stainless steel compared to its adhesion on smooth surfaces. Surfaces with features on the same scale as the S. aureus cells (1 m) appeared to promote the strongest attachment due to maximal cell-substrate contact area.…”

mentioning

confidence: 99%

Effect of Micro- and Nanoscale Topography on the Adhesion of Bacterial Cells to Solid Surfaces

Hsu

Fang

Borca-Tasciuc

et al. 2013

Appl Environ Microbiol

284

212

View full text Add to dashboard Cite

c Attachment and biofilm formation by bacterial pathogens on surfaces in natural, industrial, and hospital settings lead to infections and illnesses and even death. Minimizing bacterial attachment to surfaces using controlled topography could reduce the spreading of pathogens and, thus, the incidence of illnesses and subsequent human and financial losses. In this context, the attachment of key microorganisms, including Escherichia coli, Listeria innocua, and Pseudomonas fluorescens, to silica and alumina surfaces with micron and nanoscale topography was investigated. The results suggest that orientation of the attached cells occurs preferentially such as to maximize their contact area with the surface. Moreover, the bacterial cells exhibited different morphologies, including different number and size of cellular appendages, depending on the topographical details of the surface to which they attached. This suggests that bacteria may utilize different mechanisms of attachment in response to surface topography. These results are important for the design of novel microbe-repellant materials.

show abstract

“…Additionally, in concurrence with D. S. Rimai et al (9) , they mentioned that changes in the contact areas between the substrate and sphere are non-linear but they did not clearly mention the reasons behind this. R. D. Boyd et al (10) recognized that the roughness and surface shape of the substrate influenced the attachment and resistance against bacteria over substrate, and requested the force, which is needed for the removal of the bacteria cell, by decreasing abrading using a commercial cantilever against the bacteria cell over a stainless substrate. Also, there are reports on controlling the various surface effects in a micro contact surface, effectively.…”

Section: Introductionmentioning

confidence: 99%

Cleaning of Dust between Interactive Contact Surfaces by Application of Normal Loads of Artificial Stainless-Cantilever in AFM

Choi

Horie

Ando

2007

JAMDSM

View full text Add to dashboard Cite

In this study, we investigated that interactive contact surfaces were affected by dust and applied normal loads of cantilever such as bristles. In order to study the effect of interactive contact surfaces, spherical particles (dry borosilicate glass sphere, plastic sphere) with curvature radius(R=5µm, R=10µm) were glued to artificial stainless cantilevers (spring constant k=576.7N/m). The experiments were performed on various normal applied loads using an AFM (Atomic force microscope). The results indicate that spheres with a small curvature radius removed dust more effectively than did either of those with a large curvature radius, abraded by using the stainless cantilever, over the wide contact area (50µmx50µm). The plastic spheres tend to deform more than do the borosilicate glass spheres under the same applied load and the spheres with a smaller curvature radius tend to deform than do those with a larger curvature radius and the same material properties. Therefore, it had an influence on interactive surface forces. Restructuring dust aggregates by sliding a cantilever, as well as applying loads and contact pressure, forms a new micro contact area, which influences micro surface forces.

show abstract

Use of the Atomic Force Microscope To Determine the Effect of Substratum Surface Topography on Bacterial Adhesion

Cited by 135 publications

References 18 publications

Influence of nanophase titania topography on bacterial attachment and metabolism

Influence of nanophase titania topography on bacterial attachment and metabolism

Effect of Micro- and Nanoscale Topography on the Adhesion of Bacterial Cells to Solid Surfaces

Cleaning of Dust between Interactive Contact Surfaces by Application of Normal Loads of Artificial Stainless-Cantilever in AFM

Contact Info

Product

Resources

About