Use of tandem mass spectrometry (MS-MS) for aldosterone assay at the nanogram level in complex biological mixtures

Tandem Mass Spectrometry: The Competitive Edge for Pharmacology

“…Sensitivities are reported to be comparable to those of radioimmune assays (102,105,110), and the MSMS technique is recognized to provide much better specificity. Specificity is superior to high pressure liquid chromatogra phy and to many bioassays.…”

Preparation and Structural Elucidation of the Picolinyl Ester of Aldosterone for Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry

“…3,4) Recent trend in the development of analytical methodology for corticosteroids, liquid chromatography-mass spectrometry (LC-MS) has extensively been used in enhancing sensitivity rather than gas chromatography-mass spectrometry (GC-MS). [5][6][7][8] Aldosterone was analyzed by LC-atmospheric pressure chemical ionization (APCI)-MS 9) or LC-photospray ionization (PSI)-MS 10) with the minimum concentration of 10-15 pg/ml serum as an intact molecule (negative mode).…”

Long-term performance of a gas chromatography/tandem mass spectrometry assay for tebufelone in plasma

“…However, several groups have recently reported examples that demonstrate clear advantages of quantitative MS/MS relative to conventional MS in terms of improved limits of quantitation, reduced analysis time, and minimized sample preparation (7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22). These examples include quantitation using no chromatographic inlet (8)(9)(10)(11), a continuous flow inlet for fast atom bombardment and secondary ion MS/MS (12,13), LC/MS/MS (14,15), and GC/MS/MS (7,(16)(17)(18)(19)(20)(21)(22). Although all of these certainly demonstrate the applicability of MS/MS to trace-level quantitation in complex matrices, none have yet assessed the long-term performance of this technique upon routine application.…”