Preparation and Structural Elucidation of the Picolinyl Ester of Aldosterone for Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry

Derivatization reagents in liquid chromatography/electrospray ionization tandem mass spectrometry

“…The transition of m/z 506 ([M + H] + ) to m/z 383 was used for SRM (Honda et al ., 2007b). Picolinic acid was used for the analysis of the steroids having hydroxyl groups (Honda et al ., 2008; 2009a; Yamashita et al ., 2007a–c; 2008a–c, 2009a, b). 2‐Fluoro‐1‐methylpyridinium p ‐toluensulfonate (FPMTS) reacted with hydroxyl group at room temperature for 1 h in dichloromethane–acetonitrile containing triethylamine.…”

Highly sensitive and specific analysis of sterol profiles in biological samples by HPLC–ESI–MS/MS

“…The picolinyl derivatives showed the best HPLC-ESI-MS/MS behavior and 100-fold higher detection response by SRM compared with underivatized steroid molecules [55,56]. In addition, they have successfully applied the picolinyl derivatization to corticosteroids [57,58] and aldosterone [59,60]. Table 2.…”