Tandem Mass Spectrometry: The Competitive Edge for Pharmacology

Fenselau, Catherine

doi:10.1146/annurev.pa.32.040192.003011

Cited by 15 publications

(4 citation statements)

References 73 publications

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“…The coupling of liquid chromatography (LC) and mass spectrometry (MS) has been established as one of the most powerful tools in analytical chemistry since it debuted in the mid 1980s. The application of this technology has resulted in important advances especially in biomedical and biochemical research. , In general, the predominantly used interfaces are atmospheric pressure ionization (API) techniques such as electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI). As the ionization typically occurs on the basis of acid/base reactions, ESI and APCI are particularly well suited for the analysis of pharmaceuticals, drug metabolites, peptides, and other polar compounds.…”

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confidence: 99%

Application of On-Line Electrochemical Derivatization Coupled with High-Performance Liquid Chromatography Electrospray Ionization Mass Spectrometry for Detection and Quantitation of (p-Chlorophenyl)aniline in Biological Samples

et al. 2006

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A rapid, sensitive, and specific assay for detection and quantitation of (p-chlorophenyl)aniline (CPA) in biological samples was developed. The assay was established based on rapid electrochemical oxidation of CPA to a dimerized product (1.0 V vs Pd) with the enhanced detection sensitivity of electrospray mass spectrometer (ES/MS). A "head-to-tail" dimer ([M + H]+ at m/z 217) was exhibited as the predominant species after electrochemical conversion of CPA. Optimal detection sensitivity and specificity for the dimer of CPA that was present in the biological matrix (e.g., rat urine) were achieved through on-line electrochemistry (EC) coupled with high-performance liquid chromatography tandem mass spectrometry. No matrix-associated ion suppression was observed. The limit of detection (S/N approximately 6) was 20 ng/mL, and the limit of quantitation was 50 ng/mL. The calibration curve was exhibited to be quadratic over the range of 50-2000 ng/mL with r2 > 0.99 in various biological matrixes. The assay was validated and used to study the biotransformation of p-chlorophenyl isocyanate (CPIC) to CPA in rats administered intraperitoneally with CPIC (50 mg/kg). The present LC/EC/MS/MS assay of CPA brings important technical advantages to assist in the risk assessment of new chemical entities, which have the potential to produce anilines via biotransformation.

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confidence: 99%

Application of On-Line Electrochemical Derivatization Coupled with High-Performance Liquid Chromatography Electrospray Ionization Mass Spectrometry for Detection and Quantitation of (p-Chlorophenyl)aniline in Biological Samples

et al. 2006

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“…Interestingly, a number of drugs with a basic nitrogen moiety such as cocaine (3), methadone, (22), codeine (4), phencyclidine (6), haloperidol (9), ofloxacin (10), and nicotine (8) have all been shown to incorporate in greater amounts into hair of darker vs lighter pigmentation. At the same time the nonbasic, but nitrogen-containing drug, phenobarbital has been shown to have no preferential incorporation into hair of darker pigmentation over hair of lighter pigmentation (7).…”

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Relationship of Melanin Degradation Products to Actual Melanin Content: Application to Human Hair

Borges

Roberts

Wilkins

et al. 2001

Analytical Biochemistry

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“…c. Tandem mass spectrometry Tandem mass spectrometry developed into a routine technique by the introduction of QQQ and Q-TOF mass spectrometers, which added a new dimension of specificity to mass spectrometric analyses (for reviews see Fenselau, 1992;Costello, 1993, for a historical sketch see Jennings, 2000). By LC-MS/MS stable isotopes could now be detected/quantified also on the fragment ion level, which allows their intramolecular localization in biomolecules.…”

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confidence: 99%

A timeline of stable isotopes and mass spectrometry in the life sciences

Lehmann

2016

Mass Spec Rev

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This review retraces the role of stable isotopes and mass spectrometry in the life sciences. The timeline is divided into four segments covering the years 1920-1950, 1950-1980, 1980-2000, and 2000 until today. For each period methodic progress and typical applications are discussed. Application of stable isotopes is driven by improvements of mass spectrometry, chromatography, and related fields in sensitivity, mass accuracy, structural specificity, complex sample handling ability, data output, and data evaluation. We currently experience the vision of omics-type analyses, that is, the comprehensive identification and quantification of a complete compound class within one or a few analytical runs. This development is driven by stable isotopes without competition by radioisotopes. In metabolic studies as classic field of isotopic tracer experiments, stable isotopes and radioisotopes were competing solutions, with stable isotopes as the long-term junior partner. Since the 1990s the number of metabolic studies with radioisotopes decreases, whereas stable isotope studies retain their slow but stable upward tendency. Unique fields of stable isotopes are metabolic tests in newborns, metabolic experiments in healthy controls, newborn screening for inborn errors, quantification of drugs and drug metabolites in doping control, natural isotope fractionation in geology, ecology, food authentication, or doping control, and more recently the field of quantitative omics-type analyses. There, cells or whole organisms are systematically labeled with stable isotopes to study proteomic differences or specific responses to stimuli or genetic manipulation. The duo of stable isotopes and mass spectrometry will probably continue to grow in the life sciences, since it delivers reference-quality quantitative data with molecular specificity, often combined with informative isotope effects. © 2016 Wiley Periodicals, Inc. Mass Spec Rev 36:58-85, 2017.

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Tandem Mass Spectrometry: The Competitive Edge for Pharmacology

Cited by 15 publications

References 73 publications

Application of On-Line Electrochemical Derivatization Coupled with High-Performance Liquid Chromatography Electrospray Ionization Mass Spectrometry for Detection and Quantitation of (p-Chlorophenyl)aniline in Biological Samples

Application of On-Line Electrochemical Derivatization Coupled with High-Performance Liquid Chromatography Electrospray Ionization Mass Spectrometry for Detection and Quantitation of (p-Chlorophenyl)aniline in Biological Samples

Relationship of Melanin Degradation Products to Actual Melanin Content: Application to Human Hair

A timeline of stable isotopes and mass spectrometry in the life sciences

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