Use of sugarcane bagasse and grass hydrolysates as carbon sources for xylanase production by Bacillus circulans D1 in submerged fermentation

Bocchini, Daniela Alonso; Oliveira, Olga Maria Mascarenhas Faria; Gomes, Eleni; Silva, Roberto da

doi:10.1016/j.procbio.2005.02.021

Cited by 43 publications

(25 citation statements)

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“…Lignolytic microorganisms include fungi, actinomycetes, yeast, bacteria, and algae, all of which can produce enzymes responsible for lignocellulose degradation (Bajpai and Bajpai 1994; Annachatre and Gheewala 1996). Enzymatic treatment can be applied as a single step or with other physical and chemical methods, as extensively reviewed by Chen et al (2010), Saritha et al (2012), andBarakat et al (2014).Fungal enzymes, collectively termed as ligninases or lignin-modifying enzymes (LMEs), can degrade lignin into simple sugars and starch (Bocchini et al 2005;Dashtban et al 2010;Chopra and Singh 2012). Ligninases can be classified as phenol oxidases (laccase) or heme peroxidases (Martínez et al 2005).…”

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confidence: 99%

Wastewater Treatment and Reclamation: A Review of Pulp and Paper Industry Practices and Opportunities

Hubbe

Metts

Hermosilla

et al. 2016

BioRes

182

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The pulp and paper (P&P) industry worldwide has achieved substantial progress in treating both process water and wastewater, thus limiting the discharge of pollutants to receiving waters. This review covers a variety of wastewater treatment methods, which provide P&P companies with costeffective ways to limit the release of biological or chemical oxygen demand, toxicity, solids, color, and other indicators of pollutant load. Conventional wastewater treatment systems, often comprising primary clarification followed by activated sludge processes, have been widely implemented in the P&P industry. Higher levels of pollutant removal can be achieved by supplementary treatments, which can include anaerobic biological stages, advanced oxidation processes, bioreactors, and membrane filtration technologies. Improvements in the performance of wastewater treatment operations often can be achieved by effective measurement technologies and by strategic addition of agents including coagulants, flocculants, filter aids, and optimized fungal or bacterial cultures. In addition, P&P mills can implement upstream process changes, including dissolved-air-flotation (DAF) systems, filtration save-alls, and kidney-like operations to purify process waters, thus reducing the load of pollutants and the volume of effluent being discharged to end-of-pipe wastewater treatment plants. Keywords: Wastewater treatment; Pulp and paper manufacturing; Advanced oxidation; Membrane technologies; Clarification; Activated sludgeContact information: a: North Carolina State University, Dept. of Forest Biomaterials, Campus Box 8005, Raleigh, NC 29695-8005; b: WestRock Company, Water and Waste Treatment, 600 S 8th St, Fernandina Beach, FL 32034; c: Department of Agricultural and Forestry Engineering, University of Valladolid, Campus Duques de Soria, E-42004 Soria, Spain; d: Complutense University of Madrid, Department of Chemical Engineering, Ingn. Quim, Facultad de Ciencias Químicas, Ciudad Universitaria s/n,S-N, E-28040 Madrid, Spain; e: Concordia University, Dept. Bldg. Civil & Environm. Engn., 1455 Maisonneuve Blvd, West Montreal, PQ H3G 1M8, Canada; f: University of Jyväskylä, Dept. Chem., Box 35, FI-40014 Jyväskylä, Finland; g: Department of Biology, Center for Environmental and Marine Studies, CESAM, University of Aveiro, 3810-193 Aveiro, Portugal; h: Department of Environment and Planning, Center for Environmental and Marine Studies, CESAM, Aveiro & Department of Materials and Ceramics, Institute of Materials, CICECO, University of Aveiro, 3810-193 Aveiro, Portugal; i: FPInnovations, 570 St. Jean Blvd., Pointe Claire, PQ H9R 3J9, Canada; * Corresponding author: hubbe@ncsu.edu Contents of the Article INTRODUCTIONThe pulp and paper (P&P) industry occupies a challenging position with respect to the natural environment. On the positive side, the industry is based on the usage of renewable, photosynthetic resources. On the other hand, the industry discharges huge quantities of aqueous effluents. Large volumes (up to 70 m 3 ) of wastewater ...

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confidence: 99%

Wastewater Treatment and Reclamation: A Review of Pulp and Paper Industry Practices and Opportunities

Hubbe

Metts

Hermosilla

et al. 2016

BioRes

182

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“…Atualmente, resíduos agroindustriais têm sido utilizados como substratos para a produção de várias enzimas devido à disponibilidade local e por representarem uma fonte alternativa de baixo valor comercial 6,8,29 . Neste sentido, foi avaliada a atividade da protease secretada pelo microrganismo em estudo, quando cultivado em um meio contendo soro de queijo e água de maceração de milho.…”

Section: Utilização Do Soro De Queijo E áGua De Maceração De Milho Paunclassified

“…O uso de substratos de baixo custo, como resíduos agroindustriais, é uma alternativa para reduzir os custos de produção 6 . Dentre estes resíduos incluem-se o soro de queijo 28 e a água de maceração de milho 14 .…”

Section: Introductionunclassified

Otimização de um meio de cultura para a produção de proteases por um Bacillus sp. termofílico

Nascimento

Silva

Carvalho

et al. 2007

Ciênc. Tecnol. Aliment.

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ResumoBacillus sp. SMIA-2 cresceu e secretou proteases quando cultivado em culturas líquidas contendo citrato trissódico como fonte de carbono e nitrato de amônio como fonte de nitrogênio. A atividade máxima da enzima foi alcançada após 8 horas de incubação do microrganismo, com níveis de 7,2 U.mg -1 proteína. A suplementação do meio com 2,0 mM de CaCl 2 não proporcionou um aumento da atividade da protease, porém aumentou a sua estabilidade de 2 para 4 horas. A redução da concentração do fosfato de potássio no meio de cultura de 11,0 para 5,0 mM promoveu um acréscimo de 82% (13 U.mg -1 proteína) na atividade da protease. A substituição do nitrato de amônio presente no meio de cultura por 0,1% de soro de queijo aumentou a atividade da protease para 25 U.mg -1 proteína. Nestas condições, o tempo requerido para que a enzima atingisse seu pico de atividade máxima, que antes era de 9 horas, passou para 16 horas. A substituição do citrato trissódico do meio de cultura pela água de maceração de milho (0,5%) não só aumentou a atividade da enzima como também retardou o processo de desativação que é típico da produção de proteases. A atividade máxima da enzima obtida quando estes dois resíduos foram utilizados no meio foi 59,5 U.mg -1 proteína. Após alcançar este valor a enzima se manteve estável por mais de 20 horas o que favorece a sua produção em larga escala. Palavras-chave: proteases; Bacillus sp.; água de maceração de milho; soro de queijo. AbstractBacillus sp. SMIA-2 grew and produced proteases when cultivated in liquid cultures containing trisodium citrate as a carbon source and ammonium nitrate as a nitrogen source. The maximum activity of the enzyme was reached after incubating the microorganism for 9 hours, with levels of 7.2 U.mg -1 protein. The supplementation of the medium with 2.0 mM of CaCl 2 did not improve the activity of the protease, but increased its stability from 2 to 4 hours. The reduction in the amount of inorganic phosphate in the medium from 11.0 to 5.0 mM resulted in an improvement of 82% (13 U.mg -1 protein) in the protease activity. Replacing ammonium nitrate in the medium by 0.1% milk whey increased the protease activity to 25 U.mg -1 protein. In these conditions, the time required for the enzyme to reach a maximum activity increased from 9 to 16 hours. Replacing citrate trissodium in the medium by corn steep liquor (0.5%) not only produced much better enzyme activity, but also delayed the deactivation process which is typical for the production of proteases. The maximum activity reached when these wastes were used in the medium was 59.5 U.mg -1 protein. In addition, the enzyme maintained stable for more than 20 hours, which is favorable for its production on a large scale.

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“…Ao ser hidrolisado, o produto apresenta uma fase líquida, constituída principalmente de glicose e xilose, entre outros açúcares (Bocchini et al, 2005).…”

Section: Introductionunclassified

Variáveis que influenciam a produção de celulases e xilanase por espécies de Aspergillus

Sales

Moura

Porto

et al. 2010

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi determinar as variáveis que influenciam a produção simultânea de celulases e xilanase por Aspergillus aculeatus URM 4953 e A. phoenicis URM 4924, com uso de bagaço de cana-de-açúcar como substrato. As variáveis avaliadas foram pH inicial, tempo de cultivo, concentração do substrato, agitação, concentração do inóculo, temperatura, tipo de bagaço de cana-de-açúcar e espécie de Aspergillus. Foi utilizado o planejamento fatorial fracionário 2 , com três repetições no ponto central. Todas as variáveis analisadas influenciaram a produção de pelo menos uma das enzimas. As maiores atividades enzimáticas observadas foram: celulases totais, 0,45 UI mL . As melhores condições para produção das enzimas foram: pH 6,0; tempo de cultivo de 168 horas; bagaço de cana-de-açúcar residual como substrato; e A. aculeatus URM 4953, que produziu as celulases e a xilanase simultaneamente. As variáveis que influenciam a produção simultânea das celulases e xilanase são pH, tempo de cultivo, tipo de bagaço de cana-de-açúcar e espécie de Aspergillus.Termos para indexação: Aspergillus aculeatus, Aspergillus phoenicis, bagaço de cana-de-açúcar, celobiase, endoglucanase, exoglucanase. Variables that influence the production of cellulases and xylanase by Aspergillus speciesAbstract -The objective of this work was to determine the variables that influence the simultaneous production of cellulases and xylanase by Aspergillus aculeatus URM 4953 and A. phoenicis URM 4924, using sugarcane bagasse as substrate. Initial pH, cultivation time, substrate concentration, agitation, inoculum concentration, temperature, sugarcane bagasse type and Aspergillus species were the variables evaluated. It was used the 2 8-4fractional factorial design, with three replicates in the central point. All analysed variables influenced at least one of the enzyme production. The highest enzymatic activities were: total cellulases, 0.45 UI mL . The best conditions for enzyme production were: pH 6.0; cultivation time of 168 hours; residual sugarcane bagasse as substrate; and A. aculeatus URM 4953, which produced simultaneously all the cellulases and xylanase. The variables that influence the simultaneous production of cellulases and xylanase are pH, cultivation time, sugarcane bagasse type, and Aspergillus species.

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Use of sugarcane bagasse and grass hydrolysates as carbon sources for xylanase production by Bacillus circulans D1 in submerged fermentation

Cited by 43 publications

References 22 publications

Wastewater Treatment and Reclamation: A Review of Pulp and Paper Industry Practices and Opportunities

Wastewater Treatment and Reclamation: A Review of Pulp and Paper Industry Practices and Opportunities

Otimização de um meio de cultura para a produção de proteases por um Bacillus sp. termofílico

Variáveis que influenciam a produção de celulases e xilanase por espécies de Aspergillus

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