Use of repetitive DNA elements to define genetic relationships amongAnaplasma marginaleisolates

Abstract

Anaplasma marginale genomic DNA was tested for the presence of repetitive extragenic palindromic (REP) and enterobacterial repetitive intergenic consensus (ERIC)-like sequences in order to evaluate the genetic diversity of multiple A. marginale isolates. A. marginale isolates were obtained from cattle of six different states of Brazil, from the US and an Anaplasma centrale strain was obtained from Uruguay. Patterns obtained from A. marginale isolates varied from 14 to 17 fragments by REP-polymerase chain reaction (PCR) and 6 to 14 fragments by ERIC-PCR. All A. marginale isolates presented a 0.75-kb fragment by REP and two common fragments (0.38 and 1.0 kb) by ERIC-PCR. These two fragments were not detectable in A. centrale. Both methods produced similar patterns (80%) among A. marginale isolates obtained from the same region, although some isolates within regions shared less similarity. Isolates from Parana and Pernambuco, were differentiated by these methods. The study demonstrates the presence of ERIC and REP-like elements in A. marginale isolates and shows that A. marginale isolates and strains can be differentiated by these methods.

Genomic polymorphism of Trifolium repens root nodule symbionts from heavy metal-abundant 100-year-old waste heap in southern Poland

“…Repetitive genomic sequences, such as ERIC and REP, containing highly conserved palindromic inverted repeats are widely dispersed in bacterial genomes and have been used as a tool for the molecular typing and differentiation the bacterial strains of various genera, i.e., Mesorhizobium sp., Bacillus sp., Bordetella sp., Bartonella sp., Eschericha sp., Listeria sp., Mycobacterium sp., Salmonella sp. (Ferreira et al 2001; Sampaio et al 2006; Yang et al 2009; Gnat et al 2015). ERIC elements are 127-bp sequences which consist of highly conserved inverted repeats located in the intergenic regions whereas REP sequences are 21−65-bp palindromic units, which contain a 5-bp variable loop and are located in extragenic regions of DNA (Versalovic et al 1998; Lindsay and Sharp 2006).…”