Detection of Anaplasma marginale in Hyalomma asiaticum ticks by PCR assay

Zhang, Limei; Wang, Yong; Cai, Dongjie; He, Guangming; Cheng, Ziqiang; Liu, Jianzhu; Meng, Kai; Yang, Dawei; Wang, Shujing

doi:10.1007/s00436-013-3437-9

“…luwenshuni and T . uilenbergi ) infections were detected by PCR or nested PCR using the primers reported in previous studies [7,23–29], the detail of primers can be found in S1 Table. The primers were synthesized by Bioligo Biotechnology Co., Ltd (Shanghai, China).…”

Section: Methodsmentioning

confidence: 99%

Molecular epidemiology and risk factors of Anaplasma spp., Babesia spp. and Theileria spp. infection in cattle in Chongqing, China

Zhou

¹

,

Li

²

,

Sun

³

et al. 2019

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Tick-borne pathogens (TBPs) seriously affect cattle production and can be economically damaging. The epidemiology of these organisms in the Chongqing municipality of China is not well described. This study aimed to investigate the prevalence and risk factors of TBPs including Anaplasma spp., Babesia spp. and Theileria spp. in cattle in Chongqing municipality. The results showed that 43.48% (150/345) of cattle were infected with at least one TBP, of which single infections were detected in 104 (30.14%), double infections in 34 cattle (9.86%) and triple infections in 12 (3.48%) of the cattle. The overall prevalence of Anaplasma spp., Theileria spp. and B . bigemina were 22.32%, 23.19% and 7.24%, respectively. Among these, the prevalence of A . bovis , A . central , A . phagocytophilum , A . platys , A . marginale , T . sinensisi and T . orientalis were 8.41%, 7.83%, 4.93%, 4.35%, 2.61%, 22.32% and 2.60%, respectively. We could not detect B . bovis , T . annulata , T . luwenshuni or T . uilenbergi in cattle. Cattle ≥1-year-old were more likely to be infected with Theileria spp. [adjusted odd ratio (AOR) = 2.70, 95% CI = 1.12–6.56)] compared with younger cattle, while cattle ≥1-year-old had reduced susceptibility to B . bigemina (AOR = 0.14, 95% CI = 0.03–0.60). Cattle living at higher altitude (≥500 m) were more susceptible to B . bigemina (AOR = 6.97, 95% CI = 2.08–23.35) and Theileria spp. infection (AOR = 1.87, 95% CI = 1.06–3.32). The prevalence of Theileria spp. on farms with cats was significantly higher than that without cats (AOR = 2.56, 95% CI = 1.12–5.88). Infection with A . bovis and A . central were significantly associated with A . phagocytophilum infection. Furthermore, there were significant associations between A . bovis and A . central infection, T . sinensisi and A . marginale infection, and B . bigemina and ...

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“…In China, A. marginale was first isolated from cattle as early as 1987 in Lushi County, Henan Province [ 11 ]. Since then, A. marginale has been detected in Hyalomma asiaticum ticks and cows from five farms in northwestern China [ 13 ]. A molecular survey of Anaplasma spp.…”

Section: Discussionmentioning

confidence: 99%

Insight into the genetic diversity of Anaplasma marginale in cattle from ten provinces of China

Yang

¹

,

Ren

²

,

Liu

³

et al. 2017

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Background Anaplasma marginale is an important tick-transmitted rickettsial pathogen of cattle, with worldwide distribution and an important economic impact. The genetic diversity of A. marginale strains has been extensively characterized in different geographical regions throughout the world, while information is limited on studies in China. This study was carried out to determine the prevalence and genetic diversity of A. marginale strains in cattle from ten provinces of China.MethodsA total of 557 blood samples from cattle were collected and screened for the occurrence of A. marginale by PCR based on the msp4 gene. The partial msp1a gene containing tandem repeat sequences was further amplified from msp4 positive samples. The Msp1a amino acid repeats were identified, and genetic variation of A. marginale strains was characterized based on the variation in the repeated portion of Msp1a.ResultsOur results showed that 31.6% of 557 cattle were positive for A. marginale. The infection rates of A. marginale varied considerably from 0 to 96.9% in different sampling regions. Sequence analysis revealed that two msp4 sequence variants of A. marginale exist in cattle. One hundred and three msp1a sequences were obtained and permitted to identify 42 Msp1a tandem repeats, 21 of which were not previously published for A. marginale. Moreover, 61 A. marginale genotypes were identified based on the structure of Msp1a tandem repeats.Conclusions Anaplasma marginale is widely distributed in China and a high prevalence of infection was observed in cattle. The geographical strains of A. marginale were molecularly characterized based on the structure of Msp1a tandem repeats. Forty-two Msp1a tandem repeats and 61 genotypes of A. marginale were identified. This study, for the first time, revealed the genetic diversity of A. marginale strains in cattle in China.

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“…Infections are mainly transmitted by Rhipicephalus microplus and although most infections are subclinical, there can be fever and severe anemia resulting in production losses from decreased milk production and abortion. Studies in China have shown that the organism can be found in Rhipicephalus ( Boophilus ) microplus [28] and also that the organism might be transmitted by Hyalomma asiaticum [5]. The organism appears to be widespread in domestic ruminants in China and it has been reported to be a relatively common infection of cattle in southern and northern China [5, 15, 29, 30].…”

Section: Discussionmentioning

confidence: 99%

Molecular Detection of Anaplasma spp. and Ehrlichia spp. in Ruminants from Twelve Provinces of China

Qiu

¹

,

Kelly

²

,

Zhang

³

et al. 2016

Canadian Journal of Infectious Diseases and Medical Microbiolog

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Anaplasma spp. and Ehrlichia spp. are tick-transmitted bacteria that are of significant economic importance as they can infect large and small ruminants and also people. There is little information on anaplasmosis and ehrlichiosis in ruminants in China. 16S rRNA FRET-qPCRs were used to screen convenience whole blood samples from 2,240 domestic ruminants in 12 provinces of China for Anaplasma spp. and Ehrlichia spp. Positive samples were further analyzed with a standard PCR for the gltA. Anaplasma spp. DNA was detected in the sheep (11.7%; 13/111), goats (81.8%; 219/270), cattle (13.2%; 241/1,830), and water buffaloes (6.9%; 2/29). Ehrlichia spp. DNA was detected in sheep (1.8%; 2/111), goats (1.1%; 3/270), and cattle (3.6%; 65/1830) but not in water buffaloes (0/29). Sequencing of gltA PCR products showed that A. marginale, A. ovis, Ehrlichia canis, and Ehrlichia sp. (JX629807) were present in ruminants from China, while the 16S rRNA FRET-qPCR sequence data indicated that there might also be A. platys, A. phagocytophilum, Anaplasma sp. BL126-13 (KJ410243), and Anaplasma sp. JC3-6 (KM227012). Our study shows that domestic ruminants from China are not uncommonly infected with a variety of Anaplasma spp. and Ehrlichia spp.

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Detection of Anaplasma marginale in Hyalomma asiaticum ticks by PCR assay

Cited by 11 publications

References 19 publications

Molecular epidemiology and risk factors of Anaplasma spp., Babesia spp. and Theileria spp. infection in cattle in Chongqing, China

Molecular epidemiology and risk factors of Anaplasma spp., Babesia spp. and Theileria spp. infection in cattle in Chongqing, China

Insight into the genetic diversity of Anaplasma marginale in cattle from ten provinces of China

Molecular Detection of Anaplasma spp. and Ehrlichia spp. in Ruminants from Twelve Provinces of China

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