Insight into the genetic diversity of Anaplasma marginale in cattle from ten provinces of China

Yang, Jifei; Ren, Han; Liu, Zhijie; Niu, Qingli; Guan, Guiquan; Liu, Guangyuan; Luo, Jianxun; Yin, Hong

doi:10.1186/s13071-017-2485-x

Cited by 16 publications

(12 citation statements)

References 38 publications

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“…Consistent with our results, sequence variation at the studied gene markers has been reported previously among isolates from ticks, sheep and goats [12,[15][16][17][18][20][21][22][23][24][25]. Similarly, genetic variation is common in other Anaplasma species [40][41][42][43][44][45]. Although genetic diversity is reportedly related to infectivity, virulence, pathogenicity, niche preference, immune evasion, and/or host adaptability [46][47][48][49], this has not been established in A. capra and further studies are needed to evaluate these relationships.…”

Section: Discussionsupporting

confidence: 87%

Novel variants of the newly emerged Anaplasma capra from Korean water deer (Hydropotes inermis argyropus) in South Korea

et al. 2019

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Background: Anaplasma spp. are tick-borne Gram-negative obligate intracellular bacteria that infect humans and a wide range of animals. Anaplasma capra has emerged as a human pathogen; however, little is known about the occurrence and genetic identity of this agent in wildlife. The present study aimed to determine the infection rate and genetic profile of this pathogen in wild animals in the Republic of Korea. Methods: A total of 253 blood samples [198 from Korean water deer (Hydropotes inermis argyropus), 53 from raccoon dogs (Nyctereutes procyonoides) and one sample each from a leopard cat (Prionailurus bengalensis) and a roe deer (Capreolus pygargus)] were collected at Chungbuk Wildlife Center during the period 2015-2018. Genomic DNA was extracted from the samples and screened for presence of Anaplasma species by PCR/sequence analysis of 429 bp of the 16S rRNA gene marker. Anaplasma capra-positive isolates were genetically profiled by amplification of a longer fragment of 16S rRNA (rrs) as well as partial sequences of citrate synthase (gltA), heat-shock protein (groEL), major surface protein 2 (msp2) and major surface protein 4 (msp4). Generated sequences of each gene marker were aligned with homologous sequences in the database and phylogenetically analyzed. Results: Anaplasma capra was detected in blood samples derived from Korean water deer, whereas samples from other animal species were negative. The overall infection rate in tested samples was 13.8% (35/253) and in the water deer the rate was 17.8% (35/198), distributed along the study period from 2015 to 2018. Genetic profiling and a phylogenetic analysis based on analyzed gene markers revealed the occurrence of two distinct strains, clustered in a single clade with counterpart sequences of A. capra in the database. Conclusions: Anaplasma capra infection were detected in Korean water deer in the Republic of Korea, providing insight into the role of wildlife as a potential reservoir for animal and human anaplasmosis. However, further work is needed in order to evaluate the role of Korean water deer as a host/reservoir host of A. capra.

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Section: Discussionsupporting

confidence: 87%

Novel variants of the newly emerged Anaplasma capra from Korean water deer (Hydropotes inermis argyropus) in South Korea

et al. 2019

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“…In the previous study, Anaplasma spp. was detected in cattle in Haikou and the infection rate was 100% [24,25]. Similar results were observed in Leiqiong cattle from Hainan, in which the infection rate of A. marginale infection rate was 100%, and this infection was also detected in R. microplus.…”

Section: Discussionsupporting

confidence: 80%

“…The infection rate of A. marginale in Yunnan, Guizhou, Sichuan and Chongqing, which are located in the subtropical monsoon climate and plateau mountain climate of southwest China, is 36.6 % (67/183). In Liaoning and Inner Mongolia in Northeast China, the infection rate of A. marginale was only 9.5 % (7/74) [24]. However, these areas have obvious climate change characteristics, and the temperature gradually rises, changing into subtropical and tropical monsoon climate.…”

Section: Discussionmentioning

confidence: 99%

First Molecular Detection of Anaplasma Marginale, Babesia Bovis and Babesia Bigemina in Different Breeds of Cattle and Rhipicephalus Microplus in the Northern Hainan, China

Bhowmick

Liu

et al. 2021

Preprint

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BackgroundAnaplasma marginale, Babesia bovis and Babesia bigemina cause huge economic losses to the world's cattle industry every year. At present, there are limited investigations on A. marginale, B. bovis and B. bigemina in Hainan. The purpose of this study was to investigate the infection of A.marginale, B.bovis and B.bigemina in different breeds of cattle (Leiqiong and Wagyu), buffaloes and Rhipicephalus microplus in the northern part of Hainan.MethodsIn this study, 63 R. microplus and 151 samples of bovine blood were collected from cattle in the northern part of Hainan. A. marginale, B. bovis and B. bigemina were detected by PCR and nPCR. ResultsThe results showed that the infection rates of A. marginale, B. bovis and B. bigemina in 214 samples were 10.75% (23/214), 4.21% (9/214) and 9.35% (20/214), respectively. In Leiqiong cattle, the total infection rate of several pathogens was the highest, which was 73.33% (22/30), and there was cross-infection of several pathogens, the infection rate was 46.67% (14/30). Among them, A. marginale and B. bigemina had the highest cross-infection rate, which was 26.67% (8/30). No mixed infection was detected in R. microplus, buffalo and Wagyu. Among buffalo, the infection rates of A. marginale, B. bovis and B. bigemina were 2.17% (1/46), 6.52% (3/46) and 4.35% (2/46), respectively. A. marginale infection was only found in Wagyu, with an infection rate of 2.67% (2/75). A. marginale and B. bigemina infections were found in R. microplus, but no B. bovis infection was found. ConclusionsThe results of this study provide a reference for the formulation of prevention and control strategies for A. marginale, B. bigemina and B. bovis in Hainan.

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“…Compared to the high prevalence of A . marginale in cattle in Madagascar (89.7%), north-eastern Uganda (82.9%) [11], South Africa (57%) [31], Thailand (39.1%) [13] and in China (31.6%)[33], we demonstrated a relatively low infection rate of A . marginale (2.61%) in Chongqing.…”

Section: Discussionmentioning

confidence: 80%

Molecular epidemiology and risk factors of Anaplasma spp., Babesia spp. and Theileria spp. infection in cattle in Chongqing, China

Zhou

Sun

et al. 2019

PLoS ONE

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Tick-borne pathogens (TBPs) seriously affect cattle production and can be economically damaging. The epidemiology of these organisms in the Chongqing municipality of China is not well described. This study aimed to investigate the prevalence and risk factors of TBPs including Anaplasma spp., Babesia spp. and Theileria spp. in cattle in Chongqing municipality. The results showed that 43.48% (150/345) of cattle were infected with at least one TBP, of which single infections were detected in 104 (30.14%), double infections in 34 cattle (9.86%) and triple infections in 12 (3.48%) of the cattle. The overall prevalence of Anaplasma spp., Theileria spp. and B . bigemina were 22.32%, 23.19% and 7.24%, respectively. Among these, the prevalence of A . bovis , A . central , A . phagocytophilum , A . platys , A . marginale , T . sinensisi and T . orientalis were 8.41%, 7.83%, 4.93%, 4.35%, 2.61%, 22.32% and 2.60%, respectively. We could not detect B . bovis , T . annulata , T . luwenshuni or T . uilenbergi in cattle. Cattle ≥1-year-old were more likely to be infected with Theileria spp. [adjusted odd ratio (AOR) = 2.70, 95% CI = 1.12–6.56)] compared with younger cattle, while cattle ≥1-year-old had reduced susceptibility to B . bigemina (AOR = 0.14, 95% CI = 0.03–0.60). Cattle living at higher altitude (≥500 m) were more susceptible to B . bigemina (AOR = 6.97, 95% CI = 2.08–23.35) and Theileria spp. infection (AOR = 1.87, 95% CI = 1.06–3.32). The prevalence of Theileria spp. on farms with cats was significantly higher than that without cats (AOR = 2.56, 95% CI = 1.12–5.88). Infection with A . bovis and A . central were significantly associated with A . phagocytophilum infection. Furthermore, there were significant associations between A . bovis and A . central infection, T . sinensisi and A . marginale infection, and B . bigemina and ...

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Insight into the genetic diversity of Anaplasma marginale in cattle from ten provinces of China

Cited by 16 publications

References 38 publications

Novel variants of the newly emerged Anaplasma capra from Korean water deer (Hydropotes inermis argyropus) in South Korea

Novel variants of the newly emerged Anaplasma capra from Korean water deer (Hydropotes inermis argyropus) in South Korea

First Molecular Detection of Anaplasma Marginale, Babesia Bovis and Babesia Bigemina in Different Breeds of Cattle and Rhipicephalus Microplus in the Northern Hainan, China

Molecular epidemiology and risk factors of Anaplasma spp., Babesia spp. and Theileria spp. infection in cattle in Chongqing, China

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