Genetic diversity and molecular phylogeny of Anaplasma marginale studied longitudinally under natural transmission conditions in Rio de Janeiro, Brazil

Silva, Jenevaldo Barbosa; Gonçalves, Luiz Ricardo; Varani, Alessandro de Mello; André, Marcos Rogério; Machado, Rosangela Zacarias

doi:10.1016/j.ttbdis.2015.04.002

Cited by 24 publications

(26 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In our study, a high transplacental transmission rate of A. marginale was observed. This result has been reported in most acute experimental infections (Zaugg, 1985;Ribeiro et al, 1995;Kessler, 2001), and also in naturally infected pregnant cows (Norton et al, 1983;Ribeiro et al, 1995;Maldonado et al, 2012;Grau et al, 2013;Silva et al, 2015). Prior studies have demonstrated that transmission occurs in the second and third trimesters of pregnancy (Zaugg and Kuttler, 1984;Zaugg, 1985;Potgieter and Van Rensburg, 1987;Ribeiro et al, 1995).…”

Section: Discussionsupporting

confidence: 57%

“…The transplacental transmission of A. marginale is the most common, being described extensively in experimental studies, including longitudinal studies (Norton et al, 1983;Potgieter and Van Rensburg, 1987;Ribeiro et al, 1995;Pypers et al, 2011;Maldonado et al, 2012;Grau et al, 2013;Silva et al, 2015). It mainly occurs between the second and third trimesters of pregnancy (Fowler and Swift, 1975;Paumer, 1976, 1978;Zaugg and Kuttler, 1984;Zaugg, 1985;Potgieter and Van Rensburg, 1987;Ribeiro et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Transplacental transmission of bovine tick-borne pathogens: Frequency, co-infections and fatal neonatal anaplasmosis in a region of enzootic stability in the northeast of Brazil

Costa

Magalhães

Oliveira³

et al. 2016

Ticks and Tick-borne Diseases

Self Cite

View full text Add to dashboard Cite

Bovine tick-borne disease (TBD) constitutes a worldwide group of diseases that result in great losses for dairy and beef cattle. With regard to the epidemiological profile of the diseases, the importance of transplacental transmission is still not very well understood. The aim of this study was to determine the transplacental transmission of TBD agents (Anaplasma marginale, Babesia bovis and B. bigemina) in a herd of dairy cattle that had been naturally infected in an area of enzootic stability in northeastern Brazil. Blood for serology of the three agents was collected from cows within 120 days of gestation and serology, haemogram and nPCR assays were performed after birth. Blood was collected from the calves within 3h of birth, and haemogram and nPCR assays were performed in all animals. Pre-colostrum serology was achieved in 34 animals. The Student's t-test was used to compare the haemogram results between animals that were positive and negative for the haemoparasites. The cows were seropositive for all agents in at least one of the examinations. We detected 15 cases of vertical transmission of A. marginale, 4 of B. bovis and 2 of B. bigemina in the 60 cows. In infected animals, co-infection was detected for A. marginale and B. bovis in 1 of 60 calves, and a triple infection was detected in one other calf. Fatal neonatal anaplasmosis was observed in 1 of 15 calves, in which death occurred within 24h of birth. From the results, we concluded that transplacental transmission of TBD agents occurs, including in cases of co- and triple-infection. Such transplacental transmission can cause neonatal death, increasing the importance of this form of epidemiological transmission and suggesting its role as a cause of undiagnosed neonatal death.

show abstract

Section: Discussionsupporting

confidence: 57%

Section: Introductionmentioning

confidence: 99%

Transplacental transmission of bovine tick-borne pathogens: Frequency, co-infections and fatal neonatal anaplasmosis in a region of enzootic stability in the northeast of Brazil

Costa

Magalhães

Oliveira³

et al. 2016

Ticks and Tick-borne Diseases

Self Cite

View full text Add to dashboard Cite

show abstract

“…Therefore, the occurrence of two independent transmission mechanisms (mechanical and biological) reflects a population with high genetic diversity in endemic areas (FUENTE et al, 2001). This same hypothesis was investigated in endemic regions by Silva et al (2015a) in Brazil, where the animals were infected with multiple A. marginale strains. In the southeast and central-western regions of Brazil, where the present study was conducted, the tick R.…”

Section: Discussionmentioning

confidence: 90%

“…The global distribution and variation in pathogenicity of A. marginale are associated to the genetic diversity and variability of this bacterium (FUENTE et al, 2007). The genetic diversity of A. marginale strains is high in cattle in endemic regions worldwide FUENTE et al, 2007;CABEZAS-CRUZ et al, 2013;SILVA et al, 2015a).…”

Section: Introductionmentioning

confidence: 99%

“…In Brazil, significant variations in the MSP1a tandem repeats of strains of A. marginale have been found in the states of Paraná (VIDOTTO et al, 2006), Minas Gerais POHL et al, 2013), Rio de Janeiro (SILVA et al, 2015a) and São Paulo (SILVA et al, 2015b). In MSP1a tandem repeats of strains of A. marginale described in Brazil, the most frequent sequences are 16, α, τ in Paraná (VIDOTTO et al, 2006), 72 and α in Minas Gerais (POHL et al, 2013), 4 and τ in Rio de Janeiro (SILVA et al, 2015a) and, α and β in São Paulo (SILVA et al, 2015b). The present study aimed to understand the genetic diversity of A. marginale during an outbreak of bovine anaplasmosis in rural properties located in the municipalities of Lins, state of São Paulo (southeastern), and Mambaí, state of Goiás (central-western), Brazil.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Outbreak of anaplasmosis associated with the presence of different Anaplasma marginale strains in dairy cattle in the states of São Paulo and Goiás, Brazil

Machado

Silva

André

et al. 2015

Rev. Bras. Parasitol. Vet.

Self Cite

View full text Add to dashboard Cite

The present study reports the genetic diversity of Anaplasma marginale during anaplasmosis outbreaks in rural properties of the states of Goiás and São Paulo, Brazil. Mortality rates of 3.5% (37/1,050) in calves, 4.7% (45/954) in heifers and 1.1% (25/2,200) in lactating cows were observed in a cattle herd of the municipality of Mambaí, state of Goiás, central-western Brazil. In a cattle herd from the municipality of Lins, state of São Paulo, in southeastern Brazil, none of the animals died, despite presenting clinical signs suggestive of bovine anaplasmosis and exhibiting a drastic decrease in milk production. Thus, blood samples were collected from 100 animals with clinical signs suggestive of bovine anaplasmosis in the municipalities of Mambaí and Lins. Based on the microsatellite structure of the MSP1a of A. marginale, the genotypes E and H were observed in Lins, and the C, D and E genotypes were found in Mambaí. The analysis of the tandem repeat structures of the MSP1a showed nine different strains (τ-10 -15, α-β 2 , α-β 3 -13, α-β 2 192, τ-β-100, α-β 2 -Γ, 193-β-100, 191-13-Γ and 191-13-18) in Lins and two (α-β 3 -Γ and E-F-φ 2 -F 2 ) in Mambaí. Three new tandem repeats of MSP1a (191, 192 and 193) were described. The τ-10-15 and α-β 3 -Γ strains were predominantly associated with the occurrence of clinical anaplasmosis and mortality in calves, heifers and lactating cows.

show abstract

Correlation between Rhipicephalus microplus ticks and Anaplasma marginale infection in various cattle breeds in Brazil

et al. 2020

View full text Add to dashboard Cite

Your article is protected by copyright and all rights are held exclusively by Springer Nature Switzerland AG. This e-offprint is for personal use only and shall not be selfarchived in electronic repositories. If you wish to self-archive your article, please use the accepted manuscript version for posting on your own website. You may further deposit the accepted manuscript version in any repository, provided it is only made publicly available 12 months after official publication or later and provided acknowledgement is given to the original source of publication and a link is inserted to the published article on Springer's website. The link must be accompanied by the following text: "The final publication is available at link.springer.com".

show abstract

Genetic diversity and molecular phylogeny of Anaplasma marginale studied longitudinally under natural transmission conditions in Rio de Janeiro, Brazil

Cited by 24 publications

References 39 publications

Transplacental transmission of bovine tick-borne pathogens: Frequency, co-infections and fatal neonatal anaplasmosis in a region of enzootic stability in the northeast of Brazil

Transplacental transmission of bovine tick-borne pathogens: Frequency, co-infections and fatal neonatal anaplasmosis in a region of enzootic stability in the northeast of Brazil

Outbreak of anaplasmosis associated with the presence of different Anaplasma marginale strains in dairy cattle in the states of São Paulo and Goiás, Brazil

Correlation between Rhipicephalus microplus ticks and Anaplasma marginale infection in various cattle breeds in Brazil

Contact Info

Product

Resources

About