Use of Ovsynch in dairy herds—differences between primiparous and multiparous cows

Tenhagen, B.-A.; Surholt, Ralf; Wittke, Miriam; Vogel, C.; Drillich, M.; Heuwieser, W.

doi:10.1016/j.anireprosci.2003.08.009

Cited by 59 publications

(40 citation statements)

References 36 publications

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“…For example, Ovsynch is an effective program used to synchronise ovulation in lactating dairy cows, but it is less effective with heifers [58]. Even among lactating cows, different pregnancy rates are observed between primiparous and multiparous cows [59]. Those different success rates could be caused by small differences in follicular development, which are impossible to detect with current methods.…”

Section: Discussionmentioning

confidence: 99%

Global gene expression in granulosa cells of growing, plateau and atretic dominant follicles in cattle

Girard

Dufort

Douville

et al. 2015

Reprod Biol Endocrinol

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BackgroundThe physiological state of the dominant follicle is important as it may be linked to the competence of the oocyte within. The objective of this study was to analyze, by transcriptomic analysis, the changes occurring in granulosa cells from dominant follicles at different phases of follicular growth.MethodsGranulosa cells were collected from slaughterhouse dairy cattle follicles with a diameter greater than 9 mm, and were classified at different phases of follicle growth based on flow cytometry profiles of DNA content after staining with propidium iodide. Three phases were identified based on the proportion of cells in -G1 (less than 2n DNA), G0-G1 (2n DNA) or S-M (more than 2n DNA) and follicles were thus allocated to the growing, plateau or atresia group. Between group analysis (BGA) showed clear segregation of the three groups, and the groups were contrasted against each other in a loop design to identify differently expressed genes. Ingenuity Pathway Analysis (IPA) was used to identify the functions and upstream regulators associated with the observed differently expressed genes.ResultsMajor differences were observed between the growth phases. Granulosa cells from follicles in the plateau phase had increased expression of TYRO3 and downregulation of JAM2 compared to growing follicles, supporting the idea of a shift from proliferation to differentiation. On the other hand, genes regulating the response to oxidative stress (VNN1) and angiogenesis (ANGPT2) were upregulated in granulosa cells from atretic follicles. While the predicted activated functions in cells at the plateau stage compared to cells at the growing stage included synthesis and transport of molecules, the predictions for atretic follicles relative to plateau ones included an increase in apoptosis and cell death.ConclusionConsistent with previous studies, these observations allowed us to match the presence of specific gene transcripts to a particular physiological status and consequently to classify follicles. The results also demonstrated that the plateau phase is not a simple ‘in between’ status between growth and atresia, as several characteristics are unique to this stage.Electronic supplementary materialThe online version of this article (doi:10.1186/s12958-015-0010-7) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Global gene expression in granulosa cells of growing, plateau and atretic dominant follicles in cattle

Girard

Dufort

Douville

et al. 2015

Reprod Biol Endocrinol

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“…Pregnancy rates did not differ between the treatment groups ( p = 0.873) similar to the findings of Lopez-Gatius et al [16] who reported that synchronisation does not significantly affect pregnancy rates. Other experimental studies on synchronisation protocols reported pregnancy rates from 30% to 40% [3,13,20,23,30,32]. …”

Section: Discussionmentioning

confidence: 99%

Comparison between two progesterone sources and two oestradiol formulations in a Heatsynch protocol for postpartum cycling dairy cows in pasture

et al. 2013

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To compare an injectable progesterone (MAD-4) with an intravaginal device (IPD), and natural O17 with synthetic oestradiol (OB) in a synchronisation protocol, 51 cows were divided into four groups. Each group was treated with one of the two sources of progesterone and one of the two oestradiol formulations. Oestrus behaviour, follicle diameter, and pregnancy rates were evaluated. Oestrus behaviour (p = 0.902), numbers of cows in oestrus (p = 0.917), follicle diameter (p = 0.416), and pregnancy rates (p = 0.873) were similar among the four groups. More cows in the group treated with the IPD and OB scored > 200 oestrus behaviour points compared to the other groups (p = 0.038). A longer interval between the end of treatment and oestrus was observed among cows treated with MAD-4 than cows given the IPD (p = 0.030), but no differences were found between animals receiving the two oestradiol formulations (OB and O17). While the use of MAD-4 requires further testing, similar responses to natural oestradiol observed in the present study could allow the use of this formulation in reproductive protocols because it is not associated with the potential human health risks of OB.

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“…These responses still prove to be controversial today (Tenhagen et al, 2004). Although problematic, the possible reason for better responses of primiparous cows to fertility and synchronization protocols is that there is a lower risk of metabolic problems in early lactation (Erb and Grohn, 1988;Gröhn and Rajala-Schultz, 2000).…”

Section: Discussionmentioning

confidence: 99%

Effects of blood ²-hydroxybutyric acid levels on the response to Ovsynch in primiparous cows

Salar¹,

Baştan²

2017

Anim Reprod

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The aim of this study was to investigate the effects of low and moderate blood β-hydroxybutyric acid (BHBA) levels in primiparous cows during the early postpartum period on follicle development and pregnancy rate after the Ovsynch protocol. Holstein primiparous cows with low (n = 85) and moderate (n = 71) BHBA concentrations were used as experimental animals. At 7-8 weeks postpartum, blood samples were collected and the Ovsynch protocol was performed on the same day. Ultrasonographic examination was performed to determine the dominant follicle (DF) diameter and presence of corpus luteum on the day of the first and second gonadotropin releasing hormone (GnRH1 and GnRH2) injections and prostaglandin F2 alpha (PGF2α) injection as well as 24 and 48 h after the GnRH1 and PGF2α injections. The ovulatory response to GnRH1 and pregnancy rate at day 30 and day 60 postinsemination were also evaluated. No significant differences were observed between groups in the DF diameter on the day of GnRH1 and PGF2α injections, and at 24 and 48 h after these injections, as well as in the ovulation and atresia response to GnRH1 (P > 0.05). Significant differences were observed between groups in DF diameter (low-BHBA group, 13.8 ± 0.2 mm; moderate-BHBA group, 13.2 ± 0.2 mm; P = 0.039) at the time of insemination, and in pregnancy rates at 30 days (low-BHBA group, 38.82%; moderate-BHBA group, 21.13%, P = 0.017) and 60 days after insemination (37.6 and 18.3%, respectively; P = 0.008). Our findings suggest that moderate blood levels of BHBA (0.80-1.19 mmol/L) in primiparous cows during the early postpartum period affect the response to the Ovsynch protocol.

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Use of Ovsynch in dairy herds—differences between primiparous and multiparous cows

Cited by 59 publications

References 36 publications

Global gene expression in granulosa cells of growing, plateau and atretic dominant follicles in cattle

Global gene expression in granulosa cells of growing, plateau and atretic dominant follicles in cattle

Comparison between two progesterone sources and two oestradiol formulations in a Heatsynch protocol for postpartum cycling dairy cows in pasture

Effects of blood ²-hydroxybutyric acid levels on the response to Ovsynch in primiparous cows

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