Use of endophytic bacteria from roots of Cyperus rotundus for biocontrol of Meloidogyne incognita

Mardhiana, Mardhiana; Pradana, Ankardiansyah Pandu; Adiwena, Muh; Santoso, Dwi; Wijaya, Rizza; Murtilaksono, Aditya

doi:10.13057/biodiv/d180404

Cited by 16 publications

(10 citation statements)

References 26 publications

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“…isolated from 5271 Coffea canephora plant showed significant nematicidal activities (85.8%) against Meloidogyne incognita (Hoang et al 2020). Two endophytic bacteria isolated from Piper nigrum L. showed high inhibition (53.6% to 65.8%) against rootknot nematode in vitro, which can be applied as bio-control agents to suppress soil-borne pathogens (Wiratno et al 2019).…”

Section: Introductionmentioning

confidence: 99%

“…According to Malfanova et al (2011), Bacillus subtilis was isolated from the hogweed plant Heracleum sosnowskyi, can significantly stimulate the growth of tomato plants and protect the plant against Meloidogyne sp. Endophytic bacteria isolated from the roots of C. rotundus are capable of reducing the number of M. incognita in the roots as well as reducing the number of galls on the roots of tomato plants infected with M. incognita (Pradana et al 2017). So, it is necessary to identify the secondary metabolites produced by endophytic bacteria against Meloidogyne sp.…”

Section: Introductionmentioning

confidence: 99%

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Secondary metabolites produced by endophytic bacteria against the Root-Knot Nematode (Meloidogyne sp.)

et al. 2020

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Abstract. Maulidia V, Soesanto L, Syamsuddin, Khairan K, Hamaguchi T, Hasegawa K, Sriwati R. 2020. Secondary metabolites produced by endophytic bacteria against the Root-Knot Nematode (Meloidogyne sp.). Biodiversitas 21: 5270-5275. Endophytic bacteria live and colonize in plant tissues without causing disease to their plant host. Among several processes, these bacteria can produce secondary metabolites that can help in the defense of plant host against pathogens. This study aimed to identify endophytic bacteria as biocontrol agents against Meloidogyne sp. in tomato plants. Six endophytic bacteria candidates from the genus Pseudomonas, Arthrobacter, Bacillus, and Serratia were isolated from Solanum Lycopersicum, Psidium guajava, Pinus merkusii, Dendrocalamus asper, Albizia chinensis, and Theobroma cacao L, respectively. The average mortality of Meloidogyne sp. by endophytic bacteria was 70,27% to 95,46%. From these, B. thuringiensis AK08 produced compounds of the secondary metabolites such as flavonoid, phenol, tannins, terpenoids, steroids, saponins, and alkaloids. The best result of the average incubation period, number of galls in the root, number of nematodes at the root, and the number of nematodes in the soil on tomato plant were shown by B. thuringiensis. The major compounds in GC-MS analysis of B. thuringiensis were cholest-5-en-3-ol (3.beta.)-carbonochloridate (25.35%). Bacillus thuringiensis not only has rules as bio-insecticide but also has nematicidal effect.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Secondary metabolites produced by endophytic bacteria against the Root-Knot Nematode (Meloidogyne sp.)

et al. 2020

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“…First, in direct way by producing phytohormones, namely, auxin or cytokinin, or by producing l-aminocyclopropanel-carbon (ACC) deaminase enzyme, which reduced ethylene level in plants (Win et al 2018). Second, in indirect way by producing the plant nutrient, namely, nitrogen fixation, phosphate dilution, iron addition, and produce siderophores which can inhibit the absorption of nutrients by pathogens (Mardhiana et al 2017;Ferreira et al 2019;Munif et al 2019).…”

Section: Discussionmentioning

confidence: 99%

The potential of endophytic bacteria isolated from Tagetes sp. to control Meloidogyne spp. infection on tomato plants

2021

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Abstract. Munif A, Nursalim M, Pradana AP. 2021. The potential of endophytic bacteria isolated from Tagetes sp. to control Meloidogyne spp. infection on tomato plants. Biodiversitas 22: 3229-3236. The root-knot nematode (Meloidogyne spp.) is one of the plant-parasitic nematodes that cause economical loss in the vegetable plantations in the world. The endophytic bacteria may act as a potential biological agent to control the plant-parasitic nematodes. The aim of this study was to explore the endophytic bacteria from Tagetes sp., which had a potential to control Meloidogyne spp. and act as growth promoter for tomato plants. The endophytic bacteria were isolated from the roots and stems of Tagetes sp. and tissues were cultured on the tryptic soy agar (TSA) media. The endophytic bacterial isolates were selected using hypersensitive and blood agar tests to identify their biological safety. The selected endophytic bacteria were also assessed for growth promoter test in the tomato plants. The selected endophytic bacteria were assessed in vitro against the Meloidogyne spp. juvenile 2 and their effectiveness in suppressing the root-knot nematodes on tomato plants in greenhouse. The results showed that total of 184 endophytic bacterial isolates were successfully isolated from the roots and stem of Tagetes sp. In hypersensitive and hemolytic tests, 78 and 36 isolates showed negative responses, respectively. Of the 36 isolates tested, it was found that 14 of them were able to promote the growth of tomato seedlings. In in vitro test, 14 endophytic bacteria showed 73-93% mortality rate of Meloidogyne spp J2. The results at greenhouse showed that 3 bacterial isolates, namely, AL21, AL44, and AL53 suppress the number of root-knot nematodes up to 50-74% and improve tomato plant growth by 32%. These results indicate that endophytic bacteria isolated from Tagetes sp. have the potential to act as biocontrol agents of parasitic nematodes and a plant growth promoter.

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“…infection. Another study reported that the biological agent from rhizobacteria and endophytic bacteria groups could suppress the total of nematode eggs in tomato plant rooting system (Mardhiana et al 2017;Viljoen et al 2019).…”

Section: Discussionmentioning

confidence: 99%

Cost-effective bacteria-based bionematicide formula to control the root-knot nematode Meloidogyne spp. on tomato plants

et al. 2021

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Abstract. Asyiah IN, Prihatin J, Hastuti AD, Winarso S, Widjayanthi L, Nugroho D, Firmansyah K, Pradana AP. 2021. Cost-effective bacteria-based bionematicide formula to control root-knot nematode Meloidogyne spp. in tomato plants. Biodiversitas 22: 3256-3264. The root-knot nematode, Meloidogyne spp. can infect and cause loss production in various horticultural plants, including tomatoes. In the previous study, we found 3 endophytic bacteria isolates and 1 rhizobacterium isolate that could control several plant-parasitic nematodes. In this study, we formulated these bionematicide isolates with cheap and environmentally friendly organic materials. The formula was fortified using several organic matters, vitamin sources, protein sources, and sugar sources. The research was conducted in an experimental land with a history of severe root-knot nematode infection. The analysis showed that there were 63.7 J2 Meloidogyne spp. per 100 ml of soil on the experimental land. The application was given at a time interval of 2 weeks at the concentration of 0.5%, 1%, 1.5%, and 2%, with a dose of 100 ml per plant. As a negative control, the plant did not give any treatments, and as a positive control, the plant was given 5 g carbofuran per plant. The results revealed that treatment with 2% bionematicide formula concentration showed the best consistent result. This treatment increased canopy wet weight by 38.63% and root dry weight by 106.97% compared to negative control. The P4 treatment was also found effective to increase fruit weight by 33.61% and fruit diameter by 26.16% as compared to negative control. Increased plant growth in P4 treatment was closely related to the total of root-knot suppression and root damage intensity. In the P4 treatment, the total of root-knots and root damage intensities was 44.83% and 32.66%, respectively, compared to the negative control. This suppression also occurred in the nematode population and nematode eggs. In the P4 treatment, the total of Meloidogyne spp. J2 in soil and root was lower by 60.74% and 66.24%, respectively, compared to the negative control. A similar phenomenon also occurred in the total of eggs, which was 79.40% lower than the total of eggs in the negative control. This study provides the latest information about a cost-effective bacteria-based bionematicide formula, which is effective in suppressing Meloidogyne spp. infection in tomato, and promotes the growth and development tomato plant.

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Use of endophytic bacteria from roots of Cyperus rotundus for biocontrol of Meloidogyne incognita

Cited by 16 publications

References 26 publications

Secondary metabolites produced by endophytic bacteria against the Root-Knot Nematode (Meloidogyne sp.)

Secondary metabolites produced by endophytic bacteria against the Root-Knot Nematode (Meloidogyne sp.)

The potential of endophytic bacteria isolated from Tagetes sp. to control Meloidogyne spp. infection on tomato plants

Cost-effective bacteria-based bionematicide formula to control the root-knot nematode Meloidogyne spp. on tomato plants

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