Use of different methods for testing antioxidative activity of oregano essential oil

Kulišić, Tea; Radonić, Ani; Katalinić, Višnja; Miloš, Mladen

doi:10.1016/j.foodchem.2003.07.024

Cited by 876 publications

(615 citation statements)

References 25 publications

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“…The assay is widely used due to its simplicity, rapidity, sensitivity and reproducibility. Based on this principle, the reduction in the concentration of the DPPH solution in the presence of a hydrogen-donating antioxidant is allowed to monitor the decrease in its absorbance at a characteristic wavelength and lead to the formation of a non-radical form DPPH-H [32].…”

Section: Resultsmentioning

confidence: 99%

The Antioxidative Capacity of Kefir Produced from Goat Milk

Yilmaz‐Ersan¹,

Özcan²,

Akpinar‐Bayizit³

et al. 2016

IJCEA

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Abstract-In this present study, the antioxidant properties of kefir produced from goat milk with kefir grains were investigated. The antioxidant capacity of kefir was evaluated by assessing the DPPH (2,2-Diphenyl-1-picrylhydrazyl), the ABTS-based method [2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid)] radical-scavenging activity and ferric reducing antioxidant power (FRAP) at different stages of fermentation and storage period. Generally, the antioxidant capacity of goat milk-kefir samples was mainly dependent on the fermentation and storage period and good stability in DPPH, ABTS and FRAPS assays. During fermentation and storage, the total phenolic content in samples demonstrated significantly decreased.

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Section: Resultsmentioning

confidence: 99%

The Antioxidative Capacity of Kefir Produced from Goat Milk

Yilmaz‐Ersan¹,

Özcan²,

Akpinar‐Bayizit³

et al. 2016

IJCEA

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“…However, much of its antioxidant properties have been demonstrated in experimental in vitro systems (Aydin et al 2014). Carvacrol decreases peroxidation of phospholipid liposomes and is found to be a good scavenger of peroxyl radicals in the b-carotene bleaching (BCB) test and in DPPH and TBARS assays (Aeschbach et al 1994;Kulisic et al 2004). It has also been reported that isoprenoid such as, CAR, via posttranscriptional actions, suppressed 3-hydroxy-3-methylglutaryl CoA (HMG-CoA) reductase activity in liver, the rate limiting step for the synthesis of MDA (Akkol et al 2009).…”

Section: Discussionmentioning

confidence: 99%

Carvacrol modulates oxidative stress and decreases cell injury in pancreas of rats with acute pancreatitis

et al. 2015

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Acute pancreatitis (AP) is considered as major problem around the world and the incidence of AP is increasing. Carvacrol (CAR), a monoterpenic phenol, has good antioxidant activity. This in vivo study was designed to evaluate whether CAR provide protection against AP that developed by pancreas injury. The rats were randomised into groups to receive (I) no therapy; (II) 50 lg/kg cerulein at 1 h intervals by four intraperitonally (i.p.) injections; (III) 50, 100 and 200 mg/kg CAR by one i.p. injection; and (IV) cerulein plus CAR after 2 h of cerulein administration. 12 h later, serum samples were obtained to assess pancreatic function, the lipase and amylase values. The oxidative stress markers were evaluated by changes in the amount of lipid peroxides measured as malondialdehyde (MDA) and changes in main tissue antioxidant enzyme levels including SOD, CAT and GSH-PX. Histopathological examination was performed using scoring systems. Additionally, oxidative DNA damage was determined by measuring the increases of 8-hydroxy-deoxyguanosine (8-OH-dG) formations. We found that the increasing doses of CAR decreased AP-induced MDA and 8-OH-dG levels. Moreover, the pancreas antioxidant enzyme activities were higher than that of the rats in the AP group when compared to the AP plus CAR group. In the treatment groups, the lipase and amylase were reduced. Besides, histopathological findings in the pancreatic tissue were alleviated (p \ 0.05). We suggest that CAR could be a safe and potent new drug candidate for treating AP through its antioxidative mechanism of action for the treatment of a wide range of disorders related to pancreas.

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“…Antioxidant activity Antioxidant potential of the silver oxide nanoparticles was determined by 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) radical scavenging activity, [50] total antioxidant activity, [51] reducing power potential [52] and b-carotene bleaching activity [53] assays in a dose-dependent manner. Ascorbic acid was used as a positive control and each experiment was performed in triplicates at each concentration.…”

Section: Therapeutic Potential Of Silver Oxide Nanoparticlesmentioning

confidence: 99%

“…[52] Cytotoxic activity of biologically synthesised silver oxide nanoparticles was evaluated by brine shrimp lethality test in a dose-dependent and time-dependent manner. Silver oxide nanoparticles exhibited high cytotoxic activity against brine shrimp nauplii with an LC 50 value 85.32 mg/ml and LC 90 value 221.8 mg/ml (time: 4 hrs).…”

Section: Brine Shrimp Lethalitymentioning

confidence: 99%

A novel approach for the biosynthesis of silver oxide nanoparticles using aqueous leaf extract ofCallistemon lanceolatus(Myrtaceae) and their therapeutic potential

Ravichandran

Paluri

Kumar

et al. 2015

Journal of Experimental Nanoscience

102

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This study describes a novel biological route for the biosynthesis of silver oxide nanoparticles utilising the aqueous extract of Callistemon lanceolatus D.C. leaves. Formation of silver oxide nanoparticles was confirmed by UVÀvisible spectroscopy, Fourier transform infrared spectroscopy, scanning electron microscopeÀenergy dispersive X-ray spectroscopy and X-ray diffraction spectroscopy analysis. The biologically synthesised silver oxide nanoparticles were found to be 3À30 nm in size with spherical and hexagonal shape by high-resolution transmission electron microscope analysis. Furthermore, the biogenic silver oxide nanoparticles demonstrated significant (p < 0.05) dose-dependent antioxidant activity in various in vitro antioxidant methods. These particles also exhibited significant (p < 0.05) dosedependent and time-dependent cytotoxic activity towards brine shrimp nauplii. Moreover, the reported method is a simple, cost-effective and eco-friendly approach for the synthesis of silver oxide nanoparticles with useful pharmacological properties.

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Use of different methods for testing antioxidative activity of oregano essential oil

Cited by 876 publications

References 25 publications

The Antioxidative Capacity of Kefir Produced from Goat Milk

The Antioxidative Capacity of Kefir Produced from Goat Milk

Carvacrol modulates oxidative stress and decreases cell injury in pancreas of rats with acute pancreatitis

A novel approach for the biosynthesis of silver oxide nanoparticles using aqueous leaf extract ofCallistemon lanceolatus(Myrtaceae) and their therapeutic potential

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