This study describes a novel biological route for the biosynthesis of silver oxide nanoparticles utilising the aqueous extract of Callistemon lanceolatus D.C. leaves. Formation of silver oxide nanoparticles was confirmed by UVÀvisible spectroscopy, Fourier transform infrared spectroscopy, scanning electron microscopeÀenergy dispersive X-ray spectroscopy and X-ray diffraction spectroscopy analysis. The biologically synthesised silver oxide nanoparticles were found to be 3À30 nm in size with spherical and hexagonal shape by high-resolution transmission electron microscope analysis. Furthermore, the biogenic silver oxide nanoparticles demonstrated significant (p < 0.05) dose-dependent antioxidant activity in various in vitro antioxidant methods. These particles also exhibited significant (p < 0.05) dosedependent and time-dependent cytotoxic activity towards brine shrimp nauplii. Moreover, the reported method is a simple, cost-effective and eco-friendly approach for the synthesis of silver oxide nanoparticles with useful pharmacological properties.
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