Use of Autologous Human mesenchymal Stromal Cell/Fibrin Clot Constructs in Upper Limb Non-Unions: Long-Term Assessment

Giannotti, Stefano; Trombi, Luisa; Bottai, Vanna; Ghilardi, M; D’Alessandro, Delfo; Danti, Serena; Dell’Osso, G; Guido, Giulio; Petrini, Mario

doi:10.1371/journal.pone.0073893

Cited by 74 publications

(54 citation statements)

References 50 publications

(48 reference statements)

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“…The need for cell attachment, proliferation, and differentiation in tissue engineering requires the design of functional scaffolds, preferably bioabsorbable. Cancellous bone allograft offers a scaffold providing advantages in terms of biocompatibility and good osteoconductive and osteoinductive properties (Giannotti et al, 2013b). There was no significant difference in the mean rate of union between the treatment groups, but there was the relatively short time to achieve union in the group of composite BMAC-ACB grafting as compared to the allograft group.…”

Section: Bmac-acb Gramentioning

confidence: 90%

“…The samples were collected at the Department of Hematology. Bone marrow mesenchymal stem cells (BM-MSCs) cultures were established as previously described (Giannotti et al, 2013b;Hernigou et al, 2005a). Bone marrow aspiration concentrate (BMAC) was produced via a density gradient centrifugation using the Sorvall TM centrifuge (Thermo Scientific, MA, USA) at 3,670 rpm for 7 min.…”

Section: Isolation and Osteogenic Pre-induction Of Mscsmentioning

confidence: 99%

“…Autologous cancellous bone gra ment of many bone defects, accelerating fracture healing, and reducing complications (Giannotti et al, 2013b;Ismail et al, 2013;Ohgushi, 2014). According to the above mentioned "diamond concept", BM-MSCs provide the most appropriate cells for inducing bone repair, as they have a strong osteogenic potential and are easily obtained by culturing iliac crest aspirates (Akram et al, 2014;Gómez-Barrena et al, 2015).…”

Section: Bmac-acb Gramentioning

confidence: 99%

“…But the graft of BM-MSCs should be seen as the catalyst for bone healing and not the only determining factor for the resolution of the disease. A surgical approach still remains the most important factor (Giannotti et al, 2013b). BM-MSCs aspiration from the iliac crest contains osteoprogenitor cells and has osteogenic and osteoinductive but not osteoconductive properties (Hatzokos et al, 2011).…”

Section: Bmac-acb Gramentioning

confidence: 99%

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Autologous Bone Marrow Stem Cells combined with Allograft Cancellous Bone in Treatment of Nonunion

et al. 2015

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Abstract-Autologous cancellous bone graft is currently used as a gold standard method for treatment of bone nonunion. However, there is a limit to the amount of autologous cancellous bone that can be harvested and the donor site morbidity presents a major disadvantage to autologous bone grafting. Embedding viable cells within biological scaffolds appears to be extremely promising. The purpose of this study was to assess the outcome of autologous bone marrow stem cells combined with a cancellous bone allograft as compared to an autologous bone graft in the treatment of bone nonunion. Bone marrow aspiration concentrate (BMAC) was previously produced from bone marrow aspirate via a density gradient centrifugation. Autologous cancellous bone was harvested in 9 patients and applied to the nonunion site. In 18 patients of the clinical trial group after the debridement, the bone gaps were filled with a composite of BMAC and allograft cancellous bone chips (BMAC-ACB). Bone consolidation was obtained in 88.9%, and the mean interval between the cell transplantation and union was 4.6 ± 1.5 months in the autograft group. Bone union rate was 94.4% in group of composite BMAC-ACB implantation. The time to union in BMAC-ACB grafting group was 3.3 ± 0.90 months, and led to faster healing when compared to the autograft. A mean concentration of autologous progenitorcells was found to be 2.43 ± 1.03 (x10 6 ) CD34 + cells/ml, and a mean viability of CD34 + cells was 97.97 ± 1.47 (%). This study shows that the implantation of BMAC has presented the efficacy for treatment of nonunion and may contribute an available alternative to autologous cancellous bone graft. But large clinical application of BM-MSCs requires a more appropriate and profound scientific investigations.

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Section: Bmac-acb Gramentioning

confidence: 90%

Section: Isolation and Osteogenic Pre-induction Of Mscsmentioning

confidence: 99%

Section: Bmac-acb Gramentioning

confidence: 99%

Section: Bmac-acb Gramentioning

confidence: 99%

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Autologous Bone Marrow Stem Cells combined with Allograft Cancellous Bone in Treatment of Nonunion

et al. 2015

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“…Thus, hMPCs may play a significant role in the regenerative process when compared with hMSCs alone 12,13 . Finally, short-term osteo-induction pushes hMSCs to start their differentiation into the osteogenic lineage without losing their proliferative potential and viability 12 .…”

Section: Introductionmentioning

confidence: 99%

Mesenchymal Stromal Cell Culture and Delivery in Autologous Conditions: A Smart Approach for Orthopedic Applications

Trombi

Danti

Savelli

et al. 2016

JoVE

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Human Mesenchymal Stromal Cells (hMSCs) are cultured in vitro with different media. Limits on their use in clinical settings, however, mainly depend on potential biohazard and inflammation risks exerted by xenogeneic nutrients for their culture. Human derivatives or recombinant materials are the first choice candidates to reduce these reactions. Therefore, culture supplements and materials of autologous origin represent the best nutrients and the safest products.Here, we describe a new protocol for the isolation and culture of bone marrow hMSCs in autologous conditions -namely, patient-derived serum as a supplement for the culture medium and fibrin as a scaffold for hMSC administration. Indeed, hMSC/fibrin clot constructs could be extremely useful for several clinical applications. In particular, we focus on their use in orthopedic surgery, where the fibrin clot derived from the donor's own blood allowed effective cell delivery and nutrient/waste exchanges. To ensure optimal safety conditions, it is of the utmost importance to avoid the risks of hMSC transformation and tissue overgrowth. For these reasons, the approach described in this paper also indicates a minimally ex vivo hMSC expansion, to reduce cell senescence and morphologic changes, and short-term osteo-differentiation before implantation, to induce osteogenic lineage specification, thus decreasing the risk of subsequent uncontrolled proliferation.

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Experimental and Pilot Clinical Study of Different Tissue‐Engineered Bone Grafts Based on Calcium Phosphate, Mesenchymal Stem Cells, and Adipose‐Derived Stromal Vascular Fraction

Бозо¹,

Volozhin²,

Zorin³

et al. 2019

Bioceramics and Biocomposites

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Use of Autologous Human mesenchymal Stromal Cell/Fibrin Clot Constructs in Upper Limb Non-Unions: Long-Term Assessment

Cited by 74 publications

References 50 publications

Autologous Bone Marrow Stem Cells combined with Allograft Cancellous Bone in Treatment of Nonunion

Autologous Bone Marrow Stem Cells combined with Allograft Cancellous Bone in Treatment of Nonunion

Mesenchymal Stromal Cell Culture and Delivery in Autologous Conditions: A Smart Approach for Orthopedic Applications

Experimental and Pilot Clinical Study of Different Tissue‐Engineered Bone Grafts Based on Calcium Phosphate, Mesenchymal Stem Cells, and Adipose‐Derived Stromal Vascular Fraction

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