Experimental and Pilot Clinical Study of Different Tissue‐Engineered Bone Grafts Based on Calcium Phosphate, Mesenchymal Stem Cells, and Adipose‐Derived Stromal Vascular Fraction

Бозо, И Я; Volozhin, G.A.; Zorin, Vadim; Деев, Р. В.; Рожков, С И; Eremin, Petr S.; Toropov, Evgeniy N.; Пулин, А А; Grachev, Vasily I.; Еремин, И И; Комлев, В. С.

doi:10.1002/9781119372097.ch12

Cited by 2 publications

(1 citation statement)

References 15 publications

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“…Recent studies have shown that the use of tissue-engineered bone grafts in clinical practice can represent a promising approach for the effective restoration of bone defects [16][17][18] . However, the manufacturing of tissue-engineered bone Notably, not only BM-MNCs but also bone marrow aspirate cells seeded on implants are capable of improving bone tissue regeneration in humans [10,19,20] .…”

Section: Discussionmentioning

confidence: 99%

Minimally Manipulated Bone Marrow-Derived Cells Can Be Used for Tissue Engineering In Situ and Simultaneous Formation of Personalized Tissue Models

Baranovskii

Demchenko

et al. 2022

Bull Exp Biol Med

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Background: The use of tissue-engineered bone autografts is a promising approach for bone defects restoration. The isolation of cells and their seeding on bone autograft is usually carried out in a laboratory, requiring significant time and two separate surgical interventions. Intraoperative creation of tissueengineered bone autograft can represent a perspective solution. The aim of this study is to investigate the possibility of creation of tissue-engineered bone autograft by intraoperative enrichment of bone tissue with bone marrow-derived mononuclear cells (BM-MNCs) isolated simultaneously. Methods. Red bone marrow and autologous bone tissue (bone fragments and bone chips) of the donor were harvested intraoperatively. BM-MNCs were isolated, and bone fragments were enriched with BM-MNCs intraoperatively. Assessment of the adhesion and proliferation of BM-MNCs on bone fragments was carried out by fluorescence microscopy and histological examination. MTT assay was used to compare metabolic activity of BM-MNCs and wBMA cells seeded on bone chips. Results. Autologous bone fragments were colonized with autologous BM-MNCs isolated simultaneously in the O.R. with further adhesion and active growth of cells. When seeded on bone chips, metabolic activity of BM-MNCs was statistically significantly higher compared to wBMA cells (p-value=0.0272) on day 14. There was no difference in metabolic activity of BM-MNCs and wBMA cells cultured in nutrient medium without bone chips. Conclusion. Technically simple method of intraoperative enrichment of autologous bone fragments with BM-MNCs isolated simultaneously allowed to create tissue-engineered bone autograft in the O.R. The safety and effectiveness of intraoperatively enriched autografts should be investigated further.

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Section: Discussionmentioning

confidence: 99%

Minimally Manipulated Bone Marrow-Derived Cells Can Be Used for Tissue Engineering In Situ and Simultaneous Formation of Personalized Tissue Models

Baranovskii

Demchenko

et al. 2022

Bull Exp Biol Med

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Intraoperative enrichment of bone autograft with autologous bone marrow-derived mononuclear cells isolated simultaneously: moving towards tissue engineeringin situ

Baranovskii

B²,

Krasilnikova

et al. 2020

Preprint

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BackgroundThe use of tissue-engineered bone autografts is a promising approach for bone defects restoration. The isolation of cells and their seeding on bone autograft is usually carried out in a laboratory, requiring significant time and two separate surgical interventions. Intraoperative creation of tissue-engineered bone autograft can represent a perspective solution. The aim of this study is to investigate the possibility of creation of tissue-engineered bone autograft by intraoperative enrichment of bone tissue with bone marrow-derived mononuclear cells (BM-MNCs) isolated simultaneously.MethodsRed bone marrow and autologous bone tissue (bone fragments and bone chips) of the donor were harvested intraoperatively. BM-MNCs were isolated, and bone fragments were enriched with BM-MNCs intraoperatively. Assessment of the adhesion and proliferation of BM-MNCs on bone fragments was carried out by fluorescence microscopy and histological examination. MTT assay was used to compare metabolic activity of BM-MNCs and wBMA cells seeded on bone chips.ResultsAutologous bone fragments were colonized with autologous BM-MNCs isolated simultaneously in the O.R. with further adhesion and active growth of cells. When seeded on bone chips, metabolic activity of BM-MNCs was statistically significantly higher compared to wBMA cells (p-value=0.0272) on day 14. There was no difference in metabolic activity of BM-MNCs and wBMA cells cultured in nutrient medium without bone chips.ConclusionTechnically simple method of intraoperative enrichment of autologous bone fragments with BM-MNCs isolated simultaneously allowed to create tissue-engineered bone autograft in the O.R. The safety and effectiveness of intraoperatively enriched autografts should be investigated further.

show abstract

Experimental and Pilot Clinical Study of Different Tissue‐Engineered Bone Grafts Based on Calcium Phosphate, Mesenchymal Stem Cells, and Adipose‐Derived Stromal Vascular Fraction

Cited by 2 publications

References 15 publications

Minimally Manipulated Bone Marrow-Derived Cells Can Be Used for Tissue Engineering In Situ and Simultaneous Formation of Personalized Tissue Models

Minimally Manipulated Bone Marrow-Derived Cells Can Be Used for Tissue Engineering In Situ and Simultaneous Formation of Personalized Tissue Models

Intraoperative enrichment of bone autograft with autologous bone marrow-derived mononuclear cells isolated simultaneously: moving towards tissue engineeringin situ

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