Use of antioxidants on rainbow trout Oncorhynchus mykiss (Walbaum, 1792) sperm diluent: effects on motility and fertilizing capability

Abstract

ABSTRACT. The present investigation determined how different antioxidants incorporated into the sperm diluent for cold storage of semen affected sperm motility and spermatozoan fertility capabilities of the rainbow trout. For the evaluations, fresh semen (C) and semen that had been stored without diluents (T1) were used as control groups. The diluents were prepared using a base of UCT diluents (T2), adding grape polyphenol (0.1 g 100 mL -1 ) (T3), trolox C (0.1 g 100 mL -1 ) (T4), polyphenol (0.1 g 100 mL -1 ) plus trolox (0.1 g 100 mL -1 ) (T5), and vitamin C (0.018 g 100 mL -1 ) (T6). The incorporation of antioxidants into sperm diluents prolongs motility and fertility of rainbow trout semen. The results show that by day two, all of the treatments showed level 5 sperm motility. After seven days of storage, only T3 and T6 dropped to level 4 sperm motility. The duration of flagellate activity on this day was maximal for T3 with 36.87 ± 0.51 s and minimal for T6 with 29.78 ± 0.52 s. On day seven, fertility was maintained with no statistically significant differences between the control and T2 (92.80 ± 0.62%), T3 (83.66 ± 2.52%), T4 (90.46 ± 1.60%), T5 (83.57 ± 2.75%), and T6 (83.57 ± 2.30%). By days 10 and 17 of storage, the fertility of T1 was zero and that of T2 was significantly lower than the control group. On day 17, the highest percentage of fertilization was 97.38 ± 1.85% for T5 and the lowest value was 64.69 ± 3.76% for T2. The results allow concluding that the sperm viability of semen stored with different antioxidants is significantly prolonged. Keywords: Oncorhynchus mykiss, polyphenol, trolox C, vitamin C, fertilizing capability, sperm motility.Uso de antioxidantes en el diluyente espermático para trucha arcoiris Oncorhynchus mykiss (Walbaum, 1792): efecto en la motilidad y capacidad fecundante RESUMEN. En la presente investigación se determinó el efecto en la motilidad espermática y la fertilidad del espermatozoide de trucha arcoiris, de diferentes antioxidantes incorporados en el diluyente espermático para el almacenamiento en frío de semen. Para las evaluaciones se utilizó como control semen fresco (C) y semen almacenado sin diluir (T1), los diluyentes fueron preparados utilizando como base el diluyente UCT (T2) al que se le incorporó polifenol de uva (0,1 g 100 mL -1 ) (T3), trolox C (0,1 g 100 mL -1 ) (T4), polifenol más trolox (0,1 g 100 mL -1 + 0,1 g 100 mL -1 ), respectivamente (T5) y vitamina C (0,018 g 100 mL -1 ) (T6). La incorporación de antioxidantes en los diluyentes espermáticos, prolongan la motilidad y fertilidad del semen de trucha arcoiris. Los resultados muestran que al día dos todos los tratamientos presentaban un nivel 5 de motilidad. Después de 7 días de almacenamiento, sólo T3 y T6 bajaron a un nivel 4 de motilidad. La duración de la actividad flagelar en este día fue máxima en T3 con 36,87 ± 0,51 s y mínima en T6 con 29,78 ± 0,52 s. Al día 7 la fertilidad se mantuvo sin diferencias estadísticamente significativas con el control en T2 (92,80 ± 0,62%), T3 (83,66 ± 2,52%), T4 (90...

Example Use of Low-Cost System for Capturing the Kinetic Parameters of Sperm Cells in Atlantic Salmon (<i>Salmo salar</i>)

“…We used a haemocyter for sperm cell count, and the sample had approximately 11 × 10 −9 ± 2 cells by ml before dilution, similar to values from another study [24]. The samples show an activation time of 180 ± 10 s and decay at 50%, similar to results from a previous study [25]. The samples were agitated daily and aerated by injecting compressed oxygen to prevent mortality of the sperm cells (which are not motile for the entire conservation period) and preserved for 20 days for periodic experiments [26].…”

“…However, these programs are expensive and require specialized instruments, therefore, their use is limited. Salmon sperm cells can be activated under laboratory conditions; the duration of motility can be assessed to evaluate sperm cell quality, the observation of initial motility, and the loss of certain properties as sperm cells in the sample lose their motility [15] [25]. Measuring semen quality is crucial in order to use these cells more efficiently.…”

“…Figure 4(a) shows examples of traces registered from samples upon initial observation and after 10 and 15 days of storage in the laboratory according to conditions of oxygenation, temperature and movement. Figures 4(b)-(d) show the VCL, VSL and VAP values on different days and illustrate the significant reduction in these values that occurs when samples are preserved in low-temperature and shaken every day for oxygenation, as previous described by [25]; the kinetic properties were quantified using an optimized protocol in our laboratory. The data indicate that this easy way for the analysis of salmon sperm cell kinetics is able to distinguish changes that occur during storage and can be used to assess sperm cell quality.…”

Cryopreservation and vitrification of fish semen: a review with special emphasis on marine species

“…Their effectiveness in freshwater teleost fish was proved by Lahnsteiner (), Lahnsteiner et al . (), Lahnsteiner and Mansour () and Ubilla and Valdebenito ().…”

Antioxidant supplementation, effect on post‐thaw spermatozoan function in three sturgeon species

“…Antioxidant molecules, such as ascorbic acid (vitamin C), catalase, glutathione and cysteine, may reduce the impact of oxidative stress and thus improve quality of thawed sperm, which have been tested in vitro and in vivo, on human, bovine, ram, rabbit, horse and boar sperm (Chatterjee & Gagnon, ; Großfeld et al., ; Maxwell & Stojanov, ; Vernet, Aitken, & Drevet, ) with inconsistent results. Available data on fish are scarce (Lahnsteiner, Mansour, & Kunz, ; Martinez‐Paramoa et al., ; Ogretmen, Inanan, & Ozturk, ; Ubilla & Valdebenito, ). Our previous work demonstrated that cryopreservation resulted in significant osmotic and oxidative stress in common carp Cyprinus carpio spermatozoa (Li & Li et al., ).…”