Antioxidant supplementation, effect on post‐thaw spermatozoan function in three sturgeon species

Li, P; Xi,; Du, Hao; Qiao, XM; Zg, Liu

doi:10.1111/rda.13103

Cited by 25 publications

(22 citation statements)

References 45 publications

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“…51 Freezing and de-freezing process naturally reduces stem cells quality due to the presence of free radicals. 52,53 In one of the stages of the current study before de-freezing, it was revealed that in the presence of H 2 O 2 , HSCs treated with GFc7 growth nanofactor had higher viability.…”

Section: Dovepressmentioning

confidence: 68%

<p>GFc7 as a Smart Growth Nanofactor for ex vivo Expansion and Cryoprotection of Humans’ Hematopoietic Stem Cells</p>

Hafizi¹,

Kalanaky²,

Fakharzadeh³

et al. 2020

IJN

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Background: Nowadays, smart synthesized nanostructures have attracted wide attention in the field of stem cell nanotechnology due to their effect on different properties of stem cells. Methods: GFc7 growth nanofactor was synthesized based on nanochelating technology as an iron-containing copper chelator nanocomplex. The effect of this nanocomplex on the expansion and differentiation of hematopoietic stem cells (HSCs) as well as its performance as a cryoprotectant was evaluated in the present study. Results: The results showed that the absolute count of CD34 + and CD34 + CD38 − cells on days 4, 7, 10 and 13; the percentage of lactate dehydrogenase enzyme on the same days and CD34 + CXCR4 population on day 10 were significantly increased when they were treated with GFc7 growth nanofactor in a fetal bovine serum (FBS)-free medium. This medium also led to delayed differentiation in HSCs. One noticeable result was that CD34 + CD38 − cells cultured in an FBS medium were immediately differentiated into CD34 + CD38 + cells, while CD34 + CD38 − cells treated with GFc7 growth nanofactor in FBS medium did not show such an immediate significant differentiation. De-freezing GFc7-treated CD34 + cells, which were already frozen according to cord blood bank protocols, showed a higher percentage of cell viability and a larger number of colonies according to colony-forming cell assay as compared to control. Conclusion: It can be claimed that treating HSCs with GFc7 growth nanofactor leads to quality and quantity improvement of HSCs, both in terms of expansion in vitro and freezing and de-freezing processes.

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Section: Dovepressmentioning

confidence: 68%

<p>GFc7 as a Smart Growth Nanofactor for ex vivo Expansion and Cryoprotection of Humans’ Hematopoietic Stem Cells</p>

Hafizi¹,

Kalanaky²,

Fakharzadeh³

et al. 2020

IJN

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“…This work was the first attempt to investigate the effects of glutamine and the combination of glutamine and cysteine on post‐thaw sperm quality in catfish species. In Persian sturgeon and Belgian sturgeon, high concentrations of glutamine resulted in higher rates of sperm motility and fertilization (Aramli et al, 2016; Li et al, 2018). However, in R .…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, an effective antioxidant defence is essential for adequate spermatozoa function. Supplementation of sperm with antioxidants during cryopreservation has been proposed to minimize these harmful effects of ROS and thus to improve sperm viability (Aramli et al, 2016; Li et al, 2018; Öğretmen et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Effect of glutamine and cysteine supplementation on quality of cryopreserved sperm of South American silver catfish

Costa

Marques

Lassen

et al. 2020

Aquaculture Research

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The aim of this study was to investigate the effects of different concentrations of glutamine (experiment 1) and glutamine + cysteine (experiment 2) on post‐thaw sperm quality of South American silver catfish (Rhamdia quelen). The sperm was cryopreserved in cryoprotectant solution (fructose 50 g/L, powdered milk 50 g/L and methanol 100 ml/L) containing different glutamine concentrations (0, 2.5 and 5 mM; experiment 1) and a combination of glutamine (2.5 and 5 mM) and cysteine (2.5 and 5 mM; experiment 2). In experiment 1: glutamine supplementation groups increased DNA fragmentation; the concentration of carbonyl groups was lower and the concentration of sulfhydryl groups was higher in control; a higher activity of superoxide dismutase (SOD), catalase (CAT), glutathione‐S‐transferase (GST) and glutathione peroxidase (GPx) enzymes was observed in the 2.5 and 5 mM groups. In experiment 2: the group with 5 mM of cysteine +5 mM of glutamine presented the higher DNA damage; treatments with 5 mM of glutamine presented the higher concentration of carbonyl groups and a higher activity of CAT and GST. The findings of this study show that glutamine and cysteine, at the concentrations evaluated in this study, does not present satisfactory results but rather damaging effects on sperm quality of R. quelen.

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“…According to some studies in fish, the ROS produced during cryopreservation promote alterations in sperm cells (Balamurugan et al 2018;Figueroa et al, 2019), resulting in lipid peroxidation (Martínez-Páramo et al 2012b;Klaiwattana et al 2016;Riesco et al 2017a), DNA fragmentation (Cabrita et al 2011;Ögretmen et al 2015), mitochondrial damage and dysfunction (He & Woods 2004;Cabrita et al 2005a;Figueroa et al 2016Figueroa et al , 2019, protein oxidation (Purdy et al 2016) and loss or inactivation of enzymes associated with sperm motility (Dietrich et al 2015;Nynca et al 2015;Klaiwattana et al 2016). For this reason, the supplementation of cryopreservation media with both enzymatic and nonenzymatic antioxidants has become a common practice in cryopreservation of the semen of several fish species (Cabrita et al 2011;Lahnsteiner et al 2011;Figueroa et al 2018;Li et al 2018); nevertheless, the use of these compounds is still controversial. The objectives of this review were as follows: (i) to provide an overview of oxidative stress mechanisms and the damage caused by ROS during cryopreservation of fish spermatozoa, and (ii) to summarise recent knowledge about antioxidant defence in fish semen and antioxidant supplementation in cryopreservation media to establish perspectives for future studies.…”

Section: Introductionmentioning

confidence: 99%

“…2018; Li et al . 2018); nevertheless, the use of these compounds is still controversial. The objectives of this review were as follows: (i) to provide an overview of oxidative stress mechanisms and the damage caused by ROS during cryopreservation of fish spermatozoa, and (ii) to summarise recent knowledge about antioxidant defence in fish semen and antioxidant supplementation in cryopreservation media to establish perspectives for future studies.…”

Section: Introductionmentioning

confidence: 99%

Oxidative stress and use of antioxidants in fish semen cryopreservation

Sandoval‐Vargas

Jiménez

González

et al. 2020

Reviews in Aquaculture

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Reactive oxygen species (ROS) have been proposed as one of the main causes of the impairment of fish spermatozoa integrity and functionality during cryopreservation. The high content of unsaturated fatty acids in sperm cells and the low antioxidant capacity of diluted semen are key factors in making sperm cells susceptible to ROS attacks. For this reason, some recent studies have determined the antioxidant status of the seminal plasma and spermatozoa of fish species. Additionally, some studies have evaluated the effects of antioxidants on post-thaw sperm quality. Although ROS are certainly involved in sperm damage, other factors, such as ice crystal formation, seem to play a crucial role in cryodamage. This challenge has not yet been resolved because both the endogenous antioxidant capacity of the semen and its response to different supplementation practices seem to present specific inter-and intraspecies characteristics and effects. This review summarises knowledge on antioxidant defence and oxidative stress in fish semen, as well as antioxidant supplementation in cryopreservation media, in order to establish perspectives for future studies.

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Antioxidant supplementation, effect on post‐thaw spermatozoan function in three sturgeon species

Cited by 25 publications

References 45 publications

<p>GFc7 as a Smart Growth Nanofactor for ex vivo Expansion and Cryoprotection of Humans’ Hematopoietic Stem Cells</p>

<p>GFc7 as a Smart Growth Nanofactor for ex vivo Expansion and Cryoprotection of Humans’ Hematopoietic Stem Cells</p>

Effect of glutamine and cysteine supplementation on quality of cryopreserved sperm of South American silver catfish

Oxidative stress and use of antioxidants in fish semen cryopreservation

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