Use of anchor protein modules in fluorescence polarisation aptamer assay for ochratoxin A determination

Самохвалов, А. В.; Safenkova, Irina V.; Eremin, Sergei A.; Zherdev, Anatoly V.; Dzantiev, Boris B.

doi:10.1016/j.aca.2017.01.024

Cited by 37 publications

(30 citation statements)

References 49 publications

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“…This presented MNPs-SPEs sensor offers higher sensitivity as compared with other methods in the detection of OTA, including fluorescence methods [ 33 , 34 , 35 ], enzyme immunoassay [ 30 ], fluorescence polarization (FP) analysis [ 36 ], and other different sensor methods [ 37 , 38 , 39 , 40 , 41 ]. Compared with other ultrasensitive techniques, such as fluorescence resonance energy transfer (FRET) aptasensor [ 42 ] and impedimetric aptasensor [ 43 ], our MNPs-SPEs sensor offers a similar detection limit, but in a shorter detection time.…”

Section: Resultsmentioning

confidence: 99%

Development of a Magnetic Nanoparticles-Based Screen-Printed Electrodes (MNPs-SPEs) Biosensor for the Quantification of Ochratoxin A in Cereal and Feed Samples

Zhang

Wang

Xie

et al. 2018

Toxins

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A rapid and sensitive electrochemical biosensor based on magnetic nanoparticles and screen-printed electrodes (MNPs-SPEs sensor) was developed for the detection of ochratoxin A (OTA) in cereal and feed samples. Different types of magnetic nanoparticles-based ELISA (MNPs-ELISA) were optimized, and the signal detection, as well as sensitivity, was enhanced by the combined use of screen-printed electrodes (SPEs). Under the optimized conditions, the calibration curve of the MNPs-SPEs sensor was y = 0.3372x + 0.8324 (R2 = 0.9805). The linear range of detection and the detection limit were 0.01–0.82 ng/mL and 0.007 ng/mL, respectively. In addition, 50% inhibition (IC50) was detectable at 0.10 ng/mL. The limit of detection (LOD) of this MNPs-SPEs sensor in cereal and feed samples was 0.28 μg/kg. The recovery rates in spiked samples were between 78.7% and 113.5%, and the relative standard deviations (RSDs) were 3.6–9.8%, with the coefficient of variation lower than 15%. Parallel analysis of commercial samples (corn, wheat, and feedstuff) showed a good correlation between MNPs-SPEs sensor and liquid chromatography tandem mass spectrometry (LC/MS-MS). This new method provides a rapid, highly sensitive, and less time-consuming method to determine levels of ochratoxin A in cereal and feedstuff samples.

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Section: Resultsmentioning

confidence: 99%

Development of a Magnetic Nanoparticles-Based Screen-Printed Electrodes (MNPs-SPEs) Biosensor for the Quantification of Ochratoxin A in Cereal and Feed Samples

Zhang

Wang

Xie

et al. 2018

Toxins

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“…Fluorescence polarization (FP) or fluorescence anisotropy is a rapid, accurate, quantitative, and sensitive technique in the biochemical research in a broad range of experimental conditions [85,86,87,88,89]. The polarization value has reverberate relevance with the rotation rate of the fluorescently labeled molecule, which depends upon the molecular volume and molecular mass at a fixed solution viscosity and temperature [90].…”

Section: Fluorescence Polarization Assaymentioning

confidence: 99%

Nanotechnology based strategies for HIV-1 and HTLV-1 retroviruses gene detection

Mozhgani

Kermani

Norouzi

et al. 2020

Heliyon

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Early detection of retroviruses including human T-cell lymphotropic virus and human immunodeficiency virus in the human body is indispensable to prevent retroviral infection propagation and improve clinical treatment. Until now, diverse techniques have been employed for the early detection of viruses. Traditional methods are timeconsuming, resource-intensive, and laborious performing. Therefore, designing and constructing a selective and sensitive diagnosis system to detect serious diseases is highly demanded. Genetic detection with high sensitivity has striking significance for the early detection and remedy of disparate pathogenic diseases. The nucleic acid biosensors are based on the identification of specific DNA sequences in biological samples. Nanotechnology has an important impact on the development of sensitive biosensors. Different kinds of nanomaterials include nanoparticles, nanoclusters, quantum dots, carbon nanotubes, nanocomposites, etc., with different properties have been used to improve the performance of biosensors. Recently, DNA nanobiosensors are developed to provide simple, fast, selective, low-cost, and sensitive detection of infectious diseases. In this paper, the research progresses of nano genosensors for the detection of HIV-1 and HTLV-1 viruses, based on electrochemical, optical, and photoelectrochemical platforms are overviewed.

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“…The covalent linkage of the aptamer to the carboxylic acid group that presents on surface or nanomaterial [38] and thiolated binding aptamers to CdTe quantum dots (QDs) or Au-based materials [39]. Besides, affinity binding based on biotin-streptavidin or other affinity interaction [40,41] and hybridization of partially complementary single-stranded DNA which immobilized on surface or nanoparticle [42]. Duan et al [43] developed multicolor quantum dot nanobeads for simultaneous qualitative immunochromatographic detection of mycotoxins (ZEN, OTA, and FB 1 ) in corn samples with detection limits reached up to 5, 20, and 10 ng/mL within 10 min, respectively.…”

Section: Aptasensormentioning

confidence: 99%

Recent Biosensors Technologies for Detection of Mycotoxin in Food Products

Rovina¹,

Shaeera²,

Vonnie³

et al. 2020

Mycotoxins and Food Safety

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Mycotoxins are chemically diverse and capable of inducing a wide diversity of acute and chronic symptoms, ranging from feed refusal to rapid death. Accurate detection and monitoring of mycotoxins is an essential component of the prevention, diagnosis, and remediation of mycotoxin-related issues in livestock and human food. Current trends in food analysis are focusing on the application of fast, simple procedure needed, and low-cost biosensor technologies that can detect with high sensitivity and selectivity different compounds associated with food safety. This chapter discussed the recent analytical methods-based biosensor technology for quantification of mycotoxins in food products. Mainly focus on the biosensor technology based on the immobilization of antibodies onto various nanomaterials such as nanoparticles, graphite, carbon nanotubes, and quantum dots. The nanomaterials are able to be functionalized with various biomolecules such as enzymes, antibodies, nucleic acids, DNA/RNA aptamers, bio-or artificial receptors that make them suitable for detection of various substances such as food toxins, bacteria, and other compounds important in food analysis. All the nanomaterials provide an effective platform for achieving high sensitivity that is similar and, in some cases, even better than conventional analytical methods. We believe that future trends will be emphasized on improving biosensor properties toward practical application in the food industry.

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Use of anchor protein modules in fluorescence polarisation aptamer assay for ochratoxin A determination

Cited by 37 publications

References 49 publications

Development of a Magnetic Nanoparticles-Based Screen-Printed Electrodes (MNPs-SPEs) Biosensor for the Quantification of Ochratoxin A in Cereal and Feed Samples

Development of a Magnetic Nanoparticles-Based Screen-Printed Electrodes (MNPs-SPEs) Biosensor for the Quantification of Ochratoxin A in Cereal and Feed Samples

Nanotechnology based strategies for HIV-1 and HTLV-1 retroviruses gene detection

Recent Biosensors Technologies for Detection of Mycotoxin in Food Products

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