Use of Adipose-Derived Mesenchymal Stem Cells to Increase Viability of Composite Grafts

Yücel, Ergin; Alagöz, Murat Şahin; Eren, Guler Gamze; Yaşar, Emrah Kağan; İzmirli, Hakkı; Duruksu, Gökhan; İşgören, Serkan; Müezzínoğlu, Bahar; Karaöz, Erdal

doi:10.1097/scs.0000000000002707

Cited by 8 publications

(4 citation statements)

References 22 publications

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“…This might have occurred due to the probable incomplete action of the hyaluronidase enzyme, resulting in poorer osteoinductive and osteoconductive responses in the grafted sites, along with the presence of edema, leukocyte aggregation, and postoperative decreased blood flow, leading to the formation of a local organic barrier. Yucel et al 37 confirmed that when applied immediately after the creation of dorsal defects in rats without the presence of adipose stem cells, composite scaffolds undergo necrosis and contraction. However, when the same composites combined with mADSCs were grafted after 4 days, enhanced results were observed.…”

Section: Discussionmentioning

confidence: 95%

Adipogenic Mesenchymal Stem Cells and Hyaluronic Acid as a Cellular Compound for Bone Tissue Engineering

Boeckel

Sesterheim

Peres

et al. 2019

Journal of Craniofacial Surgery

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This study investigates the applicability of adipose mesenchymal stem cells (mADSCs) and hyaluronic acid (HA) as a cellular compound for bone tissue engineering. A critical bone defect was created on each femur of 25 rats in vivo, receiving the following 5 graft treatments: I-Control-defect; II-HA; III-mADSCs; IV-mADSCsþHA; and V-previously osteoinduced mADSCsþHA. Evaluation using microcomputed tomography, histomorphometry, and RT-PCR analysis was performed 23 days after implantation. Microcomputed tomography analysis indicated higher means of bone contact surface (BCS) and bone surface density (BSD) for the mADSCsþHA group compared with Control and the HA groups (P < 0.05). Histomorphometric findings showed higher means of bone regeneration in the mADSCsþHA compared with HA and Control groups (P < 0.05). The RT-PCR ratios showed no difference in type 1 collagen (Col1A) gene expression or osteopontin (OP) gene expression, whereas for the osteonectin gene (ON) higher means were found in the HA and mADSCs osteoinþHA groups (P < 0.05). These results suggest that a combination of HA and mADSCs without prior osteoinduction might be applicable for bone tissue regeneration.

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Section: Discussionmentioning

confidence: 95%

Adipogenic Mesenchymal Stem Cells and Hyaluronic Acid as a Cellular Compound for Bone Tissue Engineering

Boeckel

Sesterheim

Peres

et al. 2019

Journal of Craniofacial Surgery

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“…In this study, the groups treated with ADSCs showed a higher survival rate and more neovascularization than the control group; however, there was no difference depending on whether the ADSC injection was performed shortly before surgery or several days prior. In contrast, according to Yucel et al 22 , survival rates were significantly different between the group injected with ADSCs 4 days prior to composite graft surgery and the group injected immediately before surgery. It has been speculated that the raw surface stimulates the paracrine effect of ADSCs.…”

Section: Discussionmentioning

confidence: 80%

“…was performed shortly before surgery or several days prior. In contrast, according to Yucel et al [22], survival rates were significantly different between the group injected with ADSCs 4 days prior to composite graft surgery and the group injected immediately before surgery. It has been speculated that the raw surface stimulates the paracrine effect of ADSCs.…”

Section: Fig 6 Neovascularization Levelsmentioning

confidence: 80%

Effects of Human Adipose-Derived Stem Cells on the Survival of Rabbit Ear Composite Grafts

Kim

Jeon

et al. 2017

Arch Plast Surg

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BackgroundComposite grafts are frequently used for facial reconstruction. However, the unpredictability of the results and difficulties with large defects are disadvantages. Adipose-derived stem cells (ADSCs) express several cytokines, and increase the survival of random flaps and fat grafts owing to their angiogenic potential. MethodsThis study investigated composite graft survival after ADSC injection. Circular chondrocutaneous composite tissues, 2 cm in diameter, from 15 New Zealand white rabbits were used. Thirty ears were randomly divided into 3 groups. In the experimental groups (1 and 2), ADSCs were subcutaneously injected 7 days and immediately before the operation, respectively. Similarly, phosphate-buffered saline was injected in the control group just before surgery in the same manner as in group 2. In all groups, chondrocutaneous composite tissue was elevated, rotated 90 degrees, and repaired in its original position. Skin flow was assessed using laser Doppler 1, 3, 6, 9, and 12 days after surgery. At 1 and 12 days after surgery, the viable area was assessed using digital photography; the rabbits were euthanized, and immunohistochemical staining for CD31 was performed to assess neovascularization. ResultsThe survival of composite grafts increased significantly with the injection of ADSCs (P<0.05). ADSC injection significantly improved neovascularization based on anti-CD31 immunohistochemical analysis and vascular endothelial growth factor expression (P<0.05) in both group 1 and group 2 compared to the control group. No statistically significant differences in graft survival, anti-CD31 neovascularization, or microcirculation were found between groups 1 and 2. ConclusionsTreatment with ADSCs improved the composite graft survival, as confirmed by the survival area and histological evaluation. The differences according to the injection timing were not significant.

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“…ASC-based therapy has become an applicable method to prevent I/R injury for assisting flap transplantation. For instance, ASCs are able to enhance angiogenesis to increase the viability of chondrocutaneous composite grafts, for the application of defects in the nose, ear scales, and skin (Yucel et al, 2016).…”

Section: Asc-exos In Skin Flaps Injurymentioning

confidence: 99%

Exosomes From Adipose-Derived Stem Cells: The Emerging Roles and Applications in Tissue Regeneration of Plastic and Cosmetic Surgery

Xiong

Zhang

et al. 2020

Front. Cell Dev. Biol.

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Adipose-derived stem cells (ASCs) are an important stem cell type separated from adipose tissue, with the properties of multilineage differentiation, easy availability, high proliferation potential, and self-renewal. Exosomes are novel frontiers of intercellular communication regulating the biological behaviors of cells, such as angiogenesis, immune modulation, proliferation, and migration. ASC-derived exosomes (ASC-exos) are important components released by ASCs paracrine, possessing multiple biological activities. Tissue regeneration requires coordinated "vital networks" of multiple growth factors, proteases, progenitors, and immune cells producing inflammatory cytokines. Recently, as cell-to-cell messengers, ASC-exos have received much attention for the fact that they are important paracrine mediators contributing to their suitability for tissue regeneration. ASC-exos, with distinct properties by encapsulating various types of bioactive cargoes, are endowed with great application potential in tissue regeneration, mechanically via the migration and proliferation of repair cells, facilitation of the neovascularization, and other specific functions in different tissues. Here, this article elucidated the research progress of ASC-exos about tissue regeneration in plastic and cosmetic surgery, including skin anti-aging therapy, dermatitis improvement, wound healing, scar removal, flap transplantation, bone tissue repair and regeneration, obesity prevention, fat grafting, breast cancer, and breast reconstruction. Deciphering the biological properties of ASC-exos will provide further insights for exploring novel therapeutic strategies of tissue regeneration in plastic and cosmetic surgery.

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Use of Adipose-Derived Mesenchymal Stem Cells to Increase Viability of Composite Grafts

Cited by 8 publications

References 22 publications

Adipogenic Mesenchymal Stem Cells and Hyaluronic Acid as a Cellular Compound for Bone Tissue Engineering

Adipogenic Mesenchymal Stem Cells and Hyaluronic Acid as a Cellular Compound for Bone Tissue Engineering

Effects of Human Adipose-Derived Stem Cells on the Survival of Rabbit Ear Composite Grafts

Exosomes From Adipose-Derived Stem Cells: The Emerging Roles and Applications in Tissue Regeneration of Plastic and Cosmetic Surgery

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