Use of a live vaccine to modulate Cryptobia salmositica infections in Salmo salar

Davy, Christina M.; Crispo, Erika; Woo, P. T. K.

doi:10.3354/dao076045

Cited by 2 publications

(1 citation statement)

References 9 publications

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“…Although the LV does not cause disease in salmonids (e.g., Woo and Li 1990;Sitja-Babodilla and Woo 1994), it may cause a slight temporary anemia in fish (e.g., Atlantic salmon) where the strain multiplies (Ardelli and Woo 2002;Davy et al 2007). This is because of considerable release of parasite antigens with the production of antibodies (e.g., complement-fixing antibodies; Li and Woo 1995) by the fish and the formation of immune complexes on the surface membrane of red blood cells (Thomas and Woo 1988).…”

Section: Discussionmentioning

confidence: 99%

Towards a metalloprotease-DNA vaccine against piscine cryptobiosis caused by Cryptobia salmositica

2007

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Cysteine protease is a metabolic enzyme, whereas metalloprotease is the virulent factor in cryptobiosis caused by Cryptobia salmositica. Recombinant DNA vaccines were produced with the insertion of either the metalloprotease or cysteine protease gene of C. salmositica into plasmid vectors (pEGFP-N). As expected, fishes (Oncorhynchus mykiss and Salmo salar) injected intramuscularly with the metalloprotease-DNA (MP-DNA) vaccine (50 microg/fish) were consistently more anemic (lower packed cell volume, PCV) than controls (injected only with the plasmid) at 3-5 weeks post-inoculation. Also, there were no difference in PCV between fish injected with the cysteine-DNA plasmids and the controls. In addition, agglutinating antibodies against Cryptobia were detected only in the blood of MP-DNA-vaccinated fish at 5-7 weeks post-vaccination and not in cysteine-DNA plasmids and the control groups. MP-DNA-vaccinated fish when challenged with the pathogen had consistently lower parasitemia, delayed peak parasitemia, and faster recovery compared with the controls. All fish vaccinated with attenuated strain were protected when challenged with the pathogen; this positive control group confirmed that the two vaccines operate through different mechanisms.

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