Urine Is a Highly Cytotoxic Agent: Does It Influence Stem Cell Therapies in Urology?

Adamowicz, Jan; Kloskowski, Tomasz; Tworkiewicz, J.; Pokrywczyńska, Marta

doi:10.1016/j.transproceed.2012.01.128

Cited by 47 publications

(33 citation statements)

References 11 publications

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“…Previous reports indicated that if exposure time to urine is more than five hours, then hUDCs lose their viability because of remarkable decrease in nutrients contained in urine and changes in pH. [1,2,7] Furthermore, the preliminary data of the present study showed the presence of a strong correlation between donor's age and number of healthy cells in culture. For example, cells which were obtained from diabetic donors were never isolated and cultured successfully.…”

Section: Discussionsupporting

confidence: 58%

“…Additionally, we paid attention to collect urine samples within the first 4-5 hours after it had drained into catheter bag. [7] In the process of harvesting the hUDCs, six fresh urine samples (average amount of 100 mL per sample) were immediately transferred to the laboratory under sterile conditions approximately at 4°C. Each sample was centrifuged (Eppendorf Centrifuge 5417R, North America) at 500g for 5 minutes and the cell pellets were gently resuspended in sterile phosphate buffer saline (PBS) solution.…”

Section: Isolation and Cultivation Of Human Urine-derived Cellsmentioning

confidence: 99%

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Isolation and in vitro cultivation of human urine-derived cells: an alternative stem cell source

Tayhan¹,

Keleş²,

Topçu³

et al. 2017

Turkish Journal of Urology

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Objective: For in vitro tissue engineering in urology, stem cells are commonly isolated from tissue specimens obtained during open or endoscopic surgery. Within the context of the present study our aim was to isolate cells from human urine by an alternative and safe technique rather than using the indicated method. Material and methods:After human urine samples had been collected from young and healthy donors via urethral catheterization, cells were precipitated by centrifugation and cultured. Following this isolation procedure, cells were characterized by immunocytochemical method using specific antibodies.Results: When these cells were characterized by immunocytochemical methods using specific antibodies some of them were positive for mesenchymal stem cell marker CD90 while the others were labelled with urothelial marker cytokeratin 7. When all these results were taken into consideration, urothelial cells together with stem cells were observed in the urine-derived cell population. Conclusion:According to the results obtained from this study human urine may be preferred as an alternative stem cell and urothelial cell source in that this method is and easily reproducible non-invasive method.

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Section: Discussionsupporting

confidence: 58%

Section: Isolation and Cultivation Of Human Urine-derived Cellsmentioning

confidence: 99%

Isolation and in vitro cultivation of human urine-derived cells: an alternative stem cell source

Tayhan¹,

Keleş²,

Topçu³

et al. 2017

Turkish Journal of Urology

View full text Add to dashboard Cite

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“…Thick layer of the biomaterial has very good insulating properties against urine infiltration, yet thick layer of material is far harder to be digested by cells and as a consequence can trigger tissue inflammation caused by long time contact with a foreign body. The porosity, wettability and thickness of the scaffold needs to be balanced for the best performance [28].…”

Section: Discussionmentioning

confidence: 99%

Ureter Regeneration–The Proper Scaffold Has to Be Defined

et al. 2014

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The aim of this study was to compare two different acellular scaffolds: natural and synthetic, for urinary conduit construction and ureter segment reconstruction. Acellular aortic arch (AAM) and poly(L-lactide-co-caprolactone) (PLCL) were used in 24 rats for ureter reconstruction in both tested groups. Follow-up period was 4 weeks. Intravenous pyelography, histological and immunohistochemical analysis were performed. All animals survived surgical procedures. Patent uretero-conduit junction was observed only in one case using PLCL. In case of ureter segment reconstruction ureters were patent in one case using AAM and in four cases using PLCL scaffolds. Regeneration of urothelium layer and focal regeneration of smooth muscle layer was observed on both tested scaffolds. Obtained results indicates that synthetic acellular PLCL scaffolds showed better properties for ureter reconstruction than naturally derived acellular aortic arch.

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“…prions), and are easy to prepare, handle, and store. Urine is extremely cytotoxic for stem cells [20], [21]. Therefore, the separation of stem cells from the toxic environment of urine seems to be crucial for urinary bladder tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

Is the Poly (L- Lactide- Co– Caprolactone) Nanofibrous Membrane Suitable for Urinary Bladder Regeneration?

et al. 2014

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The purpose of this study was to compare: a new five-layered poly (L–lactide–co–caprolactone) (PLC) membrane and small intestinal submucosa (SIS) as a control in rat urinary bladder wall regeneration. The five-layered poly (L–lactide–co–caprolactone) membrane was prepared by an electrospinning process. Adipose tissue was harvested from five 8-week old male Wistar rats. Adipose derived stem cells (ADSCs) were seeded in a density of 3×106 cells/cm2 onto PLC membrane and SIS scaffolds, and cultured for 5-7 days in the stem cell culture medium. Twenty male Wistar rats were randomly divided into five equal groups. Augmentation cystoplasty was performed in a previously created dome defect. Groups: (I) PLC+ 3×106ADSCs; (II) SIS+ 3×106ADSCs; (III) PLC; (IV) SIS; (V) control. Cystography was performed after three months. The reconstructed urinary bladders were evaluated in H&E and Masson's trichrome staining. Regeneration of all components of the normal urinary bladder wall was observed in bladders augmented with cell-seeded SIS matrices. The urinary bladders augmented with SIS matrices without cells showed fibrosis and graft contraction. Bladder augmentation with the PLC membrane led to numerous undesirable events including: bladder wall perforation, fistula or diverticula formation, and incorporation of the reconstructed wall into the bladder lumen. The new five-layered poly (L–lactide–co–caprolactone) membrane possesses poorer potential for regenerating the urinary bladder wall compared with SIS scaffold.

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Urine Is a Highly Cytotoxic Agent: Does It Influence Stem Cell Therapies in Urology?

Cited by 47 publications

References 11 publications

Isolation and in vitro cultivation of human urine-derived cells: an alternative stem cell source

Isolation and in vitro cultivation of human urine-derived cells: an alternative stem cell source

Ureter Regeneration–The Proper Scaffold Has to Be Defined

Is the Poly (L- Lactide- Co– Caprolactone) Nanofibrous Membrane Suitable for Urinary Bladder Regeneration?

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