Is the Poly (L- Lactide- Co– Caprolactone) Nanofibrous Membrane Suitable for Urinary Bladder Regeneration?

Abstract

The purpose of this study was to compare: a new five-layered poly (L–lactide–co–caprolactone) (PLC) membrane and small intestinal submucosa (SIS) as a control in rat urinary bladder wall regeneration. The five-layered poly (L–lactide–co–caprolactone) membrane was prepared by an electrospinning process. Adipose tissue was harvested from five 8-week old male Wistar rats. Adipose derived stem cells (ADSCs) were seeded in a density of 3×106 cells/cm2 onto PLC membrane and SIS scaffolds, and cultured for 5-7 days in the stem cell culture medium. Twenty male Wistar rats were randomly divided into five equal groups. Augmentation cystoplasty was performed in a previously created dome defect. Groups: (I) PLC+ 3×106ADSCs; (II) SIS+ 3×106ADSCs; (III) PLC; (IV) SIS; (V) control. Cystography was performed after three months. The reconstructed urinary bladders were evaluated in H&E and Masson's trichrome staining. Regeneration of all components of the normal urinary bladder wall was observed in bladders augmented with cell-seeded SIS matrices. The urinary bladders augmented with SIS matrices without cells showed fibrosis and graft contraction. Bladder augmentation with the PLC membrane led to numerous undesirable events including: bladder wall perforation, fistula or diverticula formation, and incorporation of the reconstructed wall into the bladder lumen. The new five-layered poly (L–lactide–co–caprolactone) membrane possesses poorer potential for regenerating the urinary bladder wall compared with SIS scaffold.

3D composites based on the blends of chitosan and collagen with the addition of hyaluronic acid

“…Cells proliferation was compared to the control (fibroblasts cultured in basic medium). Each experiment was performed in 5 replications [31,32].…”

Application of polymeric nanofibers in soft tissues regeneration

“…Bladder augmented with adipose‐derived stem cells (ADSCs) seeded poly(lactic‐co‐caprolactone) (PLCL) (A), ADSCs seeded small intestinal submucosa (SIS) (B), unseeded PLCL (C) and unseeded SIS (D). The arrows point out the reconstructed area …”

“…Pokrywczynska et al . used a new five‐layered poly (L–lactide–co–caprolactone) (PLC) nanofibrous membrane and small intestinal submucosa (SIS) as a control in rat urinary bladder wall regeneration.…”

“…Bladder augmentation with the PLC membrane led to numerous undesirable events including: bladder wall perforation, fistula or diverticula formation, and incorporation of the reconstructed wall into the bladder lumen (Fig. ) …”

Application of Bladder Acellular Matrix in Urinary Bladder Regeneration: The State of the Art and Future Directions

“…Numerous natural and synthetic biomaterials have been used for urinary bladder reconstruction with a wide range of outcomes [ 4 ]. The most favourable material for urinary bladder reconstruction has to possess good biocompatibility, biodegradation profile, and mechanical properties, especially fatigue strength and elasticity [ 5 ]. Due to these requirements, the ideal proposition seems to be extracellular matrix-derived grafts, like bladder acellular matrix (BAM).…”