Application of Bladder Acellular Matrix in Urinary Bladder Regeneration: The State of the Art and Future Directions

Pokrywczyńska, Marta; Gubańska, Iga; Drewa, Tomasz

doi:10.1155/2015/613439

Cited by 51 publications

(45 citation statements)

References 87 publications

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“…Biological scaffolds, derived from decellularized tissues and organs, are progressively applied in regenerative surgeries . During the last decade, decellularization (DC) of biological materials including heart valves, urinary bladder, dermis, cartilage and small intestine has received significant attention . as a result, a large number of newly‐developed commercial products are available and the clinical use of decellularized scaffolds is substantially increasing.…”

Section: Introductionmentioning

confidence: 99%

“…1 During the last decade, decellularization (DC) of biological materials including heart valves, urinary bladder, dermis, cartilage and small intestine has received significant attention. [2][3][4][5][6][7]43 as a result, a large number of newly-developed commercial products are available and the clinical use of decellularized scaffolds is substantially increasing. Even successful whole organ DC has been described, indicating potential application in organ transplantation.…”

Section: Introductionmentioning

confidence: 99%

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Tissue‐engineering acellular scaffolds—The significant influence of physical and procedural decellularization factors

Starnecker¹,

König²,

Hagl³

et al. 2016

J Biomed Mater Res

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The importance of decellularized medical products has significantly increased during the last years. In this paper, we evaluated the effects of selected physical and procedural decellularization (DC) factors with the aim to systematically assess their influence on DC results. 72 porcine aortic walls (AW) were divided into three groups and exposed to a DC solution for 4 h and 8 h, either continuously or in repeated cycles. The AW were rocked (90bpm), whirled (10 l/min), sonicated (120W, 45 kHz) or exposed to a combination of these treatments, followed by 10 washing cycles. Defining successful DC as removal of nuclei while keeping an intact extracellular matrix (ECM), we equalized the efficiency to the penetration depth (PD), obtained by DAPI fluorescence and H&E staining. Additionally, we performed scanning electron microscopy (SEM), Pentachrome and Picrosirius-Red staining. Results showed that significantly higher DC depths are achieved on outer compared to inner surfaces (61 ± 7%; p < 0.001). Furthermore, the PD showed a high time dependency for all samples. Compared to continuous rocking, we achieved a significant increase in the DC efficiency through cyclic treatments ( ∼ 43%), whirling ( ∼ 19%) and sonication ( ∼ 49%). The combined treatment supported these results. In all procedures, a skeletonized but intact Collagen fibrous network was obtained as confirmed by SEM analysis. In conclusion, we systematically identified essential factors to significantly enhance DC procedures. We highly recommend considering these factors in future DC protocols. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 153-162, 2018.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Tissue‐engineering acellular scaffolds—The significant influence of physical and procedural decellularization factors

Starnecker¹,

König²,

Hagl³

et al. 2016

J Biomed Mater Res

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“…[16] Currently, the major applied materials contain decellularized amnion, bladder decellularized matrix, and small intestine submucosa. [17] …”

Section: Discussionmentioning

confidence: 99%

The histocompatibility research of hair follicle stem cells with bladder acellular matrix

Wang

et al. 2016

Medicine

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Background:Hair follicle stem cells (HFSCs) were reported to have multidirectional differentiation ability and could be differentiated into melanocytes, keratin cells, smooth muscle cells, and neurons. However, the functionality of HFSCs in bladder tissue regeneration is unknown.Methods:This study was conducted to build HFSCs vs bladder acellular matrix (BAM) complexes (HFSCs–BAM complexes) in vitro and evaluated whether HFSCs have well biocompatibility with BAM. HFSCs were separated from SD rats. BAM scaffold was prepared from the submucosa of rabbit bladder tissue. Afterwards, HFSCs were inoculated on BAM.Results:HFSCs–BAM complexes grew rapidly through inverted microscope observation. Cell growth curve showed the proliferation was in stagnate phase at 7th and 8th day. Cytotoxicity assay showed the toxicity grading of BAM was 0 or 1. Scanning electron microscopy, HE staining, and masson staining showed that cells have germinated on the surface of scaffold.Conclusion:The results provide evidence that HFSCs–BAM complexes have well biocompatibility and accumulate important experimental basis for clinical applying of tissue engineering bladder.

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“…In this regard, recent studies show that the use of BAM allografts provided with VEGF (vascular endothelial growth factor) -and bFGF (basic fibroblast growth factor) loated PLGA (polylactic-co-glycolic acid) nanoparticles can rapidly restore graft vascularization meanwhile inhibiting contracture of regenerated bladder tissue in augmentation cystoplasties (10). What seems to be also reached by incorporation of trophic growth factors, among which VEGF and NGF, into cell seeded either BAM-or smart material-based scaffolds, leading to effective graft vascularization and innervation (11,16,52).…”

Section: Reliable Difficulties About the Bladder Tissue Engineering Cmentioning

confidence: 99%

Whyever bladder tissue engineering clinical applications still remain unusual even though many intriguing technological advances have been reached?

Alberti¹

2016

Gchir

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IntroductionAbout the bladder reconstructive surgery -from bladder augmentation cystoplasty to orthotopic whole neobladder -the use of autologous bowel segments, though with the onset sometimes of both prosthetic malignancies and systemic metabolic complications, still remains the gold standard since no better alternative has been proved to be wholly reliable.With regard to such pathomorphosic and malignant complications, clinical and animal model histologic examinations on prosthetic intestinal segments may at times show a sequential pathway from chronic urine exposure-related inflammatory conditions to malignant transformation, given that phlogogenic cells can also overexpress cancerogenic cytokines. Besides the adenocarcinoma, other tumors -including polypoid adenoma, signet ring carcinoma, transitional cell carcinoma, sarcoma, lymphoma and carcinoid -may affect the intestinal urinary diversion, particularly that colonic rather than the ileal one. Preternatural histotectonic connections between ureteral transitional epithelium and paranastomotic intestinal mucosa most likely explain, through altered cell growth signalling between two dissimilar cell compartments, the prevailing appearance of malignant changes just at the uretero-intestinal suture line (1-4).Systemic metabolic imbalances of intestinal urinary diversion arise from both the chronic exposure of bowel to urine -response to excess of urinary NH4, H + , Clabsorption with developing iperchloremic acidosis, whence hypokalemia, bone demineralization with resulting hyperphosphatemia/hyperphosphaturia and phosphate urinary stone formation (that's made easier given the mucus overexpression) -and, otherwise, the reconstructive measure-due removal of ileal segment with following chologenic diarrhoea/steatorrhoea, hype-

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Application of Bladder Acellular Matrix in Urinary Bladder Regeneration: The State of the Art and Future Directions

Cited by 51 publications

References 87 publications

Tissue‐engineering acellular scaffolds—The significant influence of physical and procedural decellularization factors

Tissue‐engineering acellular scaffolds—The significant influence of physical and procedural decellularization factors

The histocompatibility research of hair follicle stem cells with bladder acellular matrix

Whyever bladder tissue engineering clinical applications still remain unusual even though many intriguing technological advances have been reached?

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