Ureter Regeneration–The Proper Scaffold Has to Be Defined

Kloskowski, Tomasz; Jundziłł, Arkadiusz; Kowalczyk, Tomasz; Nowacki, Maciej; Bodnar, Magdalena; Marszałek, Andrzej; Pokrywczyńska, Marta; Frontczak-Baniewicz, Małgorzata; Kowalewski, T. A.; Chłosta, P.; Drewa, Tomasz

doi:10.1371/journal.pone.0106023

Cited by 37 publications

(36 citation statements)

References 47 publications

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“…In our work, we compared two scaffold types: natural and synthetic [24]. Obtained results showed better regeneration of urothelium on synthetic biodegradable polymer compared to natural derived acellular blood vessel matrix [24]. It should be noted that material for artificial urinary conduit construction should be produced from components that will protect from fibrosis and calcification of urine substrates on the scaffold surface.…”

Section: Plcl -Poly (L-lactide-co-caprolactone); Pga/plga -Polyglicolmentioning

confidence: 98%

“…In all animals with acellular aortic arch conduit, atresion was observed. In a second group, in which PLCL scaffold was used in 3 cases, constructed conduits were patent at the end of follow-up (4 weeks), but only in one case intense urine flow, without the presence of pus in the urinary tract, was observed [24]. Autologous acellular aortic arch, despite easy accessibility and proper extracellular matrix composition, is unsuitable for urinary conduit construction because of its small diameter and too elastic structure, which leads to atresion about one week after the surgical procedure.…”

Section: Current Attempts In Artificial Conduit Constructionmentioning

confidence: 99%

“…Use of PLCL resulted in ureter segment reconstruction in 4 cases (n = 6), which was confirmed by urography. In that group, continuity of the ureter was preserved [24]. All currently performed attempts of experimental artificial urinary conduit construction using tissue engineering techniques are presented in Figure 1.…”

Section: Current Attempts In Artificial Conduit Constructionmentioning

confidence: 99%

“…Some studies showed no differences in seeded and unseeded group [20]; on the other hand, other researches obtained positive results only when scaffold was seeded with urothelial cells [22,23]. Dorin et al regenerated the urethra using acellular [19,20,24]. B -two ureters were anastomosed with artificial conduit; urinary bladder was removed [21,22,23].…”

Section: Future Perspectivesmentioning

confidence: 99%

“…In previous experiments, only one scaffold type was analyzed. In our work, we compared two scaffold types: natural and synthetic [24]. Obtained results showed better regeneration of urothelium on synthetic biodegradable polymer compared to natural derived acellular blood vessel matrix [24].…”

Section: Plcl -Poly (L-lactide-co-caprolactone); Pga/plga -Polyglicolmentioning

confidence: 99%

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Artificial urinary conduit construction using tissue engineering methods

Kloskowski¹,

Pokrywczyńska²,

Drewa³

2015

CEJU

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Introduction Incontinent urinary diversion using an ileal conduit is the most popular method used by urologists after bladder cystectomy resulting from muscle invasive bladder cancer. The use of gastrointestinal tissue is related to a series of complications with the necessity of surgical procedure extension which increases the time of surgery. Regenerative medicine together with tissue engineering techniques gives hope for artificial urinary conduit construction de novo without affecting the ileum. Material and methods In this review we analyzed history of urinary diversion together with current attempts in urinary conduit construction using tissue engineering methods. Based on literature and our own experience we presented future perspectives related to the artificial urinary conduit construction. Results A small number of papers in the field of tissue engineered urinary conduit construction indicates that this topic requires more attention. Three main factors can be distinguished to resolve this topic: proper scaffold construction along with proper regeneration of both the urothelium and smooth muscle layers. Conclusions Artificial urinary conduit has a great chance to become the first commercially available product in urology constructed by regenerative medicine methods.

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Section: Plcl -Poly (L-lactide-co-caprolactone); Pga/plga -Polyglicolmentioning

confidence: 98%

Section: Current Attempts In Artificial Conduit Constructionmentioning

confidence: 99%

Section: Current Attempts In Artificial Conduit Constructionmentioning

confidence: 99%

Section: Future Perspectivesmentioning

confidence: 99%

Section: Plcl -Poly (L-lactide-co-caprolactone); Pga/plga -Polyglicolmentioning

confidence: 99%

See 3 more Smart Citations

Artificial urinary conduit construction using tissue engineering methods

Kloskowski¹,

Pokrywczyńska²,

Drewa³

2015

CEJU

Self Cite

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Labeling of Collagen Type I Templates with a Naturally Derived Contrast Agent for Noninvasive MR Imaging in Soft Tissue Engineering

Janke

Güvener

Dou

et al. 2018

Adv Healthcare Materials

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In vivo monitoring of tissue-engineered constructs is important to assess their integrity, remodeling, and degradation. However, this is challenging when the contrast with neighboring tissues is low, necessitating labeling with contrast agents (CAs), but current CAs have limitations (i.e., toxicity, negative contrast, label instability, and/or inappropriate size). Therefore, a naturally derived hemin-L-lysine (HL) complex is used as a potential CA to label collagen-based templates for magnetic resonance imaging (MRI). Labeling does not change the basic characteristics of the collagen templates. When hybrid templates composed of collagen type I reinforced with degradable polymers are subcutaneously implanted in mice, longitudinal visualization by MRI is possible with good contrast and in correlation with template remodeling. In contrast, unlabeled collagen templates are hardly detectable and the fate of these templates cannot be monitored by MRI. Interestingly, tissue remodeling and vascularization are enhanced within HL-labeled templates. Thus, HL labeling is presented as a promising universal imaging marker to label tissue-engineered implants for MRI, which additionally seems to accelerate tissue regeneration.

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Decellularized Scaffolds with Double‐Layer Aligned Microchannels Induce the Oriented Growth of Bladder Smooth Muscle Cells: Toward Urethral and Ureteral Reconstruction

Cheng,

Zhang,

Zhang

et al. 2023

Adv Healthcare Materials

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There is a great clinical need for regenerating urinary tissue. Native urethras and ureters have bidirectional aligned smooth muscle cells (SMCs) layers, which plays a pivotal role in micturition and transporting urine and inhibiting reflux. Thus far, urinary scaffolds have not been designed to induce the native‐mimicking aligned arrangement of SMCs. In this study, a tubular decellularized extracellular matrix (dECM) with an intact internal layer and bidirectional aligned microchannels in the tubular wall, which is realized by the subcutaneous implantation of a template, followed by the removal of the template, and decellularization, is engineered. The dense and intact internal layer effectively increases the leakage pressure of the tubular dECM scaffolds. Rat‐derived dECM scaffolds with three different sizes of microchannels are fabricated by tailoring the fiber diameter of the templates. The rat‐derived dECM scaffolds exhibiting microchannels of ≈65 µm show suitable mechanical properties, good ability to induce the bidirectional alignment and growth of human bladder SMCs, and elevated higher functional protein expression in vitro. These data indicate that rat‐derived tubular dECM scaffolds manifesting double‐layer aligned microchannels may be promising candidates to induce the native‐mimicking regeneration of SMCs in urethra and ureter reconstruction.

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Ureter Regeneration–The Proper Scaffold Has to Be Defined

Cited by 37 publications

References 47 publications

Artificial urinary conduit construction using tissue engineering methods

Artificial urinary conduit construction using tissue engineering methods

Labeling of Collagen Type I Templates with a Naturally Derived Contrast Agent for Noninvasive MR Imaging in Soft Tissue Engineering

Decellularized Scaffolds with Double‐Layer Aligned Microchannels Induce the Oriented Growth of Bladder Smooth Muscle Cells: Toward Urethral and Ureteral Reconstruction

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