Urinary tract infections caused by staphylococcus aureus DNA in comparison to the candida albicans DNA

Al-Mathkhury, Harith Jabbar Fahad; Abdul-Ghaffar, Saba Nazeih

doi:10.4297/najms.2011.3562

Cited by 4 publications

(3 citation statements)

References 19 publications

(28 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They can be easily inoculated into the urethra from the surrounding skin during anal cleaning after defecation. Similar result had been reported [26]. The other bacteria Salmonella spp and Pseudomonas aeruginosa had also been reported to cause UTI [26-28].…”

Section: Discussionsupporting

confidence: 86%

Bacteriology and Antibiogram of Urinary Tract Infection Among Female Patients in a Tertiary Health Facility in South Eastern Nigeria

Oli¹,

Akabueze²,

Ezeudu³

et al. 2017

TOMICROJ

View full text Add to dashboard Cite

Background:Urinary Tract Infection (UTI) is a common contagion among men and women with the incidence relatively higher among women due to their differing anatomy. An understanding of the kind of pathogens implicated in urinary tract infections as well as antibiotic susceptibility profiling may help the clinician make rationally correct empirical choice in their treatment.Objective:This study is aimed at determining the type and antibiotic susceptibility pattern of bacterial uropathogens isolated from female patients attending Chukwuemeka Odumegwu Ojukwu University Teaching Hospital (COOUTH), Awka, Nigeria.Method:Two hundred and forty patients with clinically diagnosed UTI and who were on at least 5 days’ antibiotic holiday were recruited into the study. Their demographic characteristics were captured using pre-tested questionnaire. Their clean catch mid-stream urine samples were collected using sterile universal container and sent to the Microbiology Department for processing. Within 30 minutes of samples collection, the specimens were cultured and the isolates were identified, after 24 h of incubation, using standard microbiological techniques. Antibiotic susceptibility tests were done with standard antibiotic discs using the Kirby–bauer disc diffusion method.Results:Out of the 240 urine samples, 89.17% yielded significant bacteriuria. The pathogens implicated were Escherichia coli (28.5%), Staphylococcus aureus (28.0%), Salmonella spp (22.8%) and Pseudomonas aeruginosa (20.5%). HIV status, patients age, pregnancy status and marital status all significantly affected bacteriuria rate (p value < 0.05), while patients’ location (sub-urban/rural dwelling), and level of education did not (p value > 0.05). The pattern of microbial resistance to antibiotics suggests that ceftazidime, fosfomycin and cefoxitin may not be used as first-line agents in the empirical treatment of UTIs rather; levofloxacin, meropenem or aztreonam should be considered. Levofloxacin was significantly effective against all the isolates and may be administered empirically while waiting for the culture result (Mean % susceptibility was 79.85).Conclusion: E. coli and S. aureus were the predominant pathogens in the study and many were resistant to the commonly prescribed antibiotics and so leave the clinicians with only few alternative drugs for UTIs treatment. Routine surveillance and monitoring studies need to be constantly conducted to update clinicians on the prevalent pathogens and the rational and empirical treatment of UTIs. Aggressive and consistent health education using every possible media is also recommended to combat the menace of drug resistance occasioned by inappropriate antibiotic use.

show abstract

Section: Discussionsupporting

confidence: 86%

Bacteriology and Antibiogram of Urinary Tract Infection Among Female Patients in a Tertiary Health Facility in South Eastern Nigeria

Oli¹,

Akabueze²,

Ezeudu³

et al. 2017

TOMICROJ

View full text Add to dashboard Cite

show abstract

“…The age of UTI patients ranged between 16-74 years with a mean of 49.84, as shown in Table-1. This result partially agrees with a study in Iraq that investigated UTI patients with an age ranged between 15-50 years [18,19]. While the age of wound patients ranged between 18-66 years with a mean of 42.04.…”

Section: -Study Populationsupporting

confidence: 90%

Detection of icaA Gene Expression in Clinical Biofilm-Producing Staphylococcus Aureus Isolates

Mohammed

Radif

2020

eijs

View full text Add to dashboard Cite

The pathogenicity resulting from Staphylococcus aureus infection has remarkable importance as one of the community-associated bacterial infections, due to the virulent ability of these bacteria to produce biofilms. This study was designed to detect biofilm production in clinical isolates from samples of wounds and urinary tract infections. The expression levels of the icaA gene that is responsible of slime layer production in biofilms was compared in isolates with different biofilm producing capabilities. Fifty seven samples that included 32 samples from urine and 25 samples from wounds were collected from Alwasti Hospital, Al-Kindi Teaching Hospital, and Alzahraa Clinic, Baghdad, Iraq. The bacteria was identified according to biochemical tests, API20 strip test, and PCR assay. The results of 16S rRNA PCR detection revealed that nine isolates were identified as S. aureus. The biofilm assay showed that 46.15% of the isolates were strong biofilm producers, 46.15% had moderate ability to produce biofilm, and 7.70% were weak producers. Quantitative PCR assay was carried out on three isolates with different biofilm-producing abilities. The results demonstrated that the strong biofilm-producing isolates had significantly higher (P ≤0.01) gene expression level (6.508) compared with the moderate (1.624) and the weak (1.231) isolates.

show abstract

“…Higher desquamation of cell associated with S. aureus and E. coli infection observed in this study (table 1) could be adduced to the bacterial adhesion molecule. The observed pleomorphic cells and higher nuclear/cytoplasmic ratio (figures 1 and 3, respectively) could be due to bacterial and candidal DNA integration into the human genome [22]. Cytoplasmic vacuolation (clear cell; figure 1) is characteristic of a lesion which is usually associated with cystitis [23] while the papillary lesion (figure 2) is associated with pyelonephritis.…”

Section: Discussionmentioning

confidence: 99%

Urethelial Dysplasia and Desquamation Associated with Urinary Tract Infection: A Case-controlled Study

Okoye¹,

Joel²,

Onyenze³

2016

ujmr

View full text Add to dashboard Cite

This study explored use of urine cytology as an early first-line approach in the laboratory diagnosis of dysplasia associated with urinary tract infection. Fifty (50) patients attending the obstetrics and gynaecology clinic at Madonna University Teaching Hospital and 50 apparently healthy subjects were recruited for the study. Urine samples were cultured and analyzed using the dipstick and Giemsa methods. The result of this study revealed significant higher number of nitrite positive samples, bacteria and pus cells when female test subjects were compared with apparently healthy female subjects (p<0.05). The frequency of Staphylococcus aureus, Escherichia coli, Streptococcus faecalis and C. albicans identified is 50.6, 27.3, 10.4 and 9.1 %, respectively while other isolated Coliforms amounted to 2.6%. Microscopy revealed marked cytolyses, desquamation and abnormal nuclear/cytoplasmic ratio. S. aureus and E. coli induced higher exfoliation and morphological change (72.7%) compared to other isolates. In conclusion, this study suggests that chronic bacterial infection could lead to accelerated desquamation of cells and urothelial carcinoma. More so, it revealed that females are at a higher risk of developing epithelial neoplasia following UTI when compared to the males. Thus, urine smears could be used as first line approach in the early diagnosis of urothelial malignancy.

show abstract

Urinary tract infections caused by staphylococcus aureus DNA in comparison to the candida albicans DNA

Cited by 4 publications

References 19 publications

Bacteriology and Antibiogram of Urinary Tract Infection Among Female Patients in a Tertiary Health Facility in South Eastern Nigeria

Bacteriology and Antibiogram of Urinary Tract Infection Among Female Patients in a Tertiary Health Facility in South Eastern Nigeria

Detection of icaA Gene Expression in Clinical Biofilm-Producing Staphylococcus Aureus Isolates

Urethelial Dysplasia and Desquamation Associated with Urinary Tract Infection: A Case-controlled Study

Contact Info

Product

Resources

About