The pathogenicity resulting from Staphylococcus aureus infection has remarkable importance as one of the community-associated bacterial infections, due to the virulent ability of these bacteria to produce biofilms. This study was designed to detect biofilm production in clinical isolates from samples of wounds and urinary tract infections. The expression levels of the icaA gene that is responsible of slime layer production in biofilms was compared in isolates with different biofilm producing capabilities. Fifty seven samples that included 32 samples from urine and 25 samples from wounds were collected from Alwasti Hospital, Al-Kindi Teaching Hospital, and Alzahraa Clinic, Baghdad, Iraq. The bacteria was identified according to biochemical tests, API20 strip test, and PCR assay. The results of 16S rRNA PCR detection revealed that nine isolates were identified as S. aureus. The biofilm assay showed that 46.15% of the isolates were strong biofilm producers, 46.15% had moderate ability to produce biofilm, and 7.70% were weak producers. Quantitative PCR assay was carried out on three isolates with different biofilm-producing abilities. The results demonstrated  that the strong biofilm-producing isolates had significantly higher (P ≤0.01) gene expression level (6.508) compared with the moderate (1.624) and the weak (1.231) isolates.

The volatile oil is metabolites product from some plant have biological activities such as antimicrobial ,antioxidant and have viability for reduced cancer cell ,through inhibit cell proliferation ,for this reason This study was set to extraction volatile oils from Inflorescences of Qusiqualis indica plant cultivation in Iraq from tow season (spring ,autumn)and identification of the Chemical compounds of these  ( using GC-MS ) ,The cytotoxic effect of two seasons volatile oils on human lung cancer cells A549  compared with normal REF cell cells were studied at concentrations (5,6,12,25,50)μl/ml and the programmed cell death (apoptosis) tested for cells lines  and measured P53 gene ,Caspase-8 expression levels. The results showed significant  quantitative and quantitative in two seasons oils  ,and there were significant differences in the degree of cytotoxicity between the two oils ,where is at the concentration (50 μl/ml)they have the highest effects of(A549)cells  compared with REF cells ,while the higher  apoptosis effect at (25 μl/ml) concentrate  for A549 compared with REF.