Uptake of polyamines by the unicellular green alga Chlamydomonas reinhardtii and their effect on ornithine decarboxylase activity

Theiss, Christine; Bohley, Peter; Bisswanger, Hans; Voigt, J.

doi:10.1078/0176-1617-00987

Cited by 18 publications

(9 citation statements)

References 42 publications

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“…The increase in the levels of free Spd and Spm might be due to their uptake and/or the transformation between them. The decrease in free Put level, however, can be explained by exogenous PAs leading to a decrease in the activity of ornithine decarboxylase (EC 4.1.1.17), a key enzyme in Put synthesis (Theiss et al, 2004). Free Spd and Spm were associated with small molecules or macromolecules, thereby resulting in the increase in PSconjugated PA levels as well as PIS-bound PA levels.…”

Section: Discussionmentioning

confidence: 98%

Exogenous polyamines enhance copper tolerance of Nymphoides peltatum

Wang

Shi

et al. 2007

Journal of Plant Physiology

117

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Section: Discussionmentioning

confidence: 98%

Exogenous polyamines enhance copper tolerance of Nymphoides peltatum

Wang

Shi

et al. 2007

Journal of Plant Physiology

117

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“…Polyamines were able to disrupt several metabolic functions within the cells by inhibition of protein synthesis accompanied by an irreversible dissociation of ribosomes [23]. The cytotoxic effects were observed in Chlamydomonas reinhardtii when exogenous putrescine was higher than 1.5 mM [31]. Indeed, we found that Synechocystis cells grown in the presence of 1.0 mM spermidine could accumulate a high content of spermidine regardless of salt-stress condition (insets of Figs.…”

Section: Discussionmentioning

confidence: 68%

Characterization of Spermidine Transport System in a Cyanobacterium,Synechocystis sp. PCC 6803

Raksajit

Yodsang²,

Mäenpää³

et al. 2009

J.Microbiol.Biotechnol

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The transport of spermidine into a cyanobacterium, Synechocystis sp. PCC 6803, was characterized by measuring the uptake of 14 C-spermidine. Spermidine transport was shown to be saturable with an apparent affinity constant (K m ) value of 67 µM and a maximal velocity (V max ) value of 0.45 nmol/min/mg protein. Spermidine uptake was pHdependent with the pH optimum being 8.0. The competition experiment showed strong inhibition of spermidine uptake by putrescine and spermine, whereas amino acids were hardly inhibitory. The inhibition kinetics of spermidine transport by putrescine and spermine was found to be noncompetitive with K i values of 292 and 432 µM, respectively. The inhibition of spermidine transport by various metabolic inhibitors and ionophores suggests that spermidine uptake is energy-dependent. The diminution of cell growth was observed in cells grown at a high concentration of NaCl. Addition of a low concentration of spermidine at 0.5 mM relieved growth inhibition by salt stress. Upshift of the external osmolality generated by either NaCl or sorbitol caused an increased spermidine transport with about 30-40% increase at 10 mosmol/kg upshift.

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“…Urea is a stage of catabolism of nitrogen bases and amino acids and may also function as an osmolyte [35]. Putrescine is the key component in the synthesis of various polyamines reg ulating the fission rate and the activity of physiological processes in Chlamydomonas [36]. It is also involved in the adaptation processes in plants, and its pool can be associated with the stability of a culture in the station ary growth phase.…”

Section: Resultsmentioning

confidence: 99%

Analysis of metabolic profile of Chlamydomonas reinhardtii cultivated under autotrophic conditions

Puzanskiy

Шаварда

Tarakhovskaya

et al. 2014

Appl Biochem Microbiol

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This study presents a metabolite profile analysis of unicellular green alga Chlamydomonas rein hardtii grown under autotrophic conditions at late stages of culture development. Metabolites were identified by gas chromatography coupled to mass spectrometry. Approximately 400 peaks corresponding to individual compounds were distinguished, of which approximately 100 compounds (including saccharides, fatty acids, aromatic compounds, amino acids, alcohols, etc.) were identified. A local database of mass spectra of uni dentified compounds was created using MassBank software. Mapping of the metabolomic data with the use of the ChlamyCyc service showed that the identified compounds are involved in various energetic, synthetic, and signaling pathways in Chlamydomonas. The mapping of metabolites by their chemical structure with the use of Cytoscape software, combined with quantitative interpretation, showed that the majority of organic matter was concentrated primarily in the carbon backbones of fatty acids and terpenes, as well as saccharides and structurally similar compounds.

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Uptake of polyamines by the unicellular green alga Chlamydomonas reinhardtii and their effect on ornithine decarboxylase activity

Cited by 18 publications

References 42 publications

Exogenous polyamines enhance copper tolerance of Nymphoides peltatum

Exogenous polyamines enhance copper tolerance of Nymphoides peltatum

Characterization of Spermidine Transport System in a Cyanobacterium,Synechocystis sp. PCC 6803

Analysis of metabolic profile of Chlamydomonas reinhardtii cultivated under autotrophic conditions

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