Uptake of oxytetracycline and its phytotoxicity to alfalfa (Medicago sativa L.)

Kong, Weidong; Zhu, Yan; Liang, Yongchao; Zhang, J.; Smith, F. A.; Yang, Mingxing

doi:10.1016/j.envpol.2006.08.016

Cited by 179 publications

(74 citation statements)

References 36 publications

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“…To further distinguish active transport from facilitated diffusion, metabolic inhibitors were employed to affect active uptake by disabling cellular energy production. 2,4-dinitrophenol (2,4-DNP) is considered primarily as metabolic inhibitor (Kong et al 2007;Zhan et al 2010;Wang et al 2011). It is regarded as an uncoupler of oxidative phosphorylation, and of proton-coupled fluxes at the plasma membrane and endomembrane via the dissipation of trans-membrane electrochemical gradients of protons (Kong et al 2007).…”

Section: Discussionmentioning

confidence: 99%

“…Both glycerol and silver are widely used to assay aquaporin activity (Kong et al 2007), but their inhibitory mechanisms differ greatly. Silver may react with the sulfhydryl group of a cysteine in the vicinity of the conserved NPA motif, thus effectively blocks the constriction region of the water channel.…”

Section: Discussionmentioning

confidence: 99%

“…0.1 mmolL −1 sodium azide (NaN 3 ) or 0.6 mmolL −1 sodium vanadate (Na 3 VO 4 ) was applied as metabolic inhibitors according to the methods reported with some modifications (Thompson et al 2000;Krapfenbauer et al 2003;Herrmann et al 2003). 1 μmolL −1 AgNO 3 or 1 mmolL −1 glycerol was used as aquaporin inhibitors (Kong et al 2007). 10 μmolL −1 anthracene-9-carboxylic acid (9-AC, Aladdin Chemistry, Co. Ltd, China), or 5 μmolL −1 4,4′-diisothiocyanostibene-2,2′-disulfonate (DIDS, Tokyo Chemical Industry, Co. Ltd, Japan), or 5 μmolL −1 5-nitro 2-(3-phenylpropylamine) benzoic acid (NPPB, Tokyo Chemical Industry, Co. Ltd, Japan) was used as anion channel inhibitors (You et al 2010;Matveyeva et al 2003).…”

Section: Concentration-dependent Uptake Of Pfosmentioning

confidence: 99%

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Mechanistic studies of perfluorooctane sulfonate, perfluorooctanoic acid uptake by maize (Zea mays L. cv. TY2)

et al. 2013

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Background and aims Perfluorinated compounds (PFCs) are of particular environmental concern. The migration of PFCs from soil to plants is a likely pathway for PFCs to enter the human food chain. This study aimed to investigate the uptake mechanisms of perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) by maize (Zea mays L. cv. TY2).Methods Hydroponic greenhouse experiments were performed. Results The kinetics of PFOS and PFOA uptake fitted Mechaelis-Menten equation well, suggesting their carrier-mediated influx processes. Uptake of PFOS was insensitive to metabolic inhibitors (NaN 3 and Na 3 VO 4 ). In contrast, treated with NaN 3 and Na 3 VO 4 reduced the uptake of PFOA by 83 and 43 % respectively. PFOS uptake was decreased by 31 % and 25 % when plants were treated with aquaporin inhibitors, AgNO 3 and glycerol, respectively, while aquaporin inhibitors had no effect on PFOA uptake. Anion channel blockers, 4, 4′-diisothiocyanostibene-2,2′-disolfonate (DID) and 5-nitro 2-(3-phenylpropylamine) benzoic acid (NPPB) inhibited the uptake of PFOS by 33 % and 30 %, respectively. Anion channel blocker anthracene-9-carboxylic acid (9-AC) decreased the uptake of PFOA by 28 %. No competitive uptake was found between PFOS and PFOA. Conclusions Uptake of PFOS and PFOA by maize may have different mechanisms.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Concentration-dependent Uptake Of Pfosmentioning

confidence: 99%

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Mechanistic studies of perfluorooctane sulfonate, perfluorooctanoic acid uptake by maize (Zea mays L. cv. TY2)

et al. 2013

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“…Few prior studies have investigated wastewater irrigation as a continuous exposure route for the co-uptake of antimicrobials and pathogens into food crops. Similarly, prior studies of antimicrobial uptake were performed in hydroponic systems (Herklotz et al, 2010, Kong et al, 2007, Migliore et al, 2003and Wu et al, 2012 or by introducing antimicrobials via soil amendment (Boxall et al, 2006, Grote et al, 2007, Kumar et al, 2005, Sabourin et al, 2012and Seo et al, 2010.…”

Section: Introductionmentioning

confidence: 99%

Concomitant uptake of antimicrobials and Salmonella in soil and into lettuce following wastewater irrigation

Sallach

Zhang

Hodges

et al. 2015

Environmental Pollution

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“…OTC can significantly affect soil microbial activity and high concentrations can produce visual symptoms in plants including light green or yellow colours in the leaves of alfalfa (Boleas et al 2005;Kong et al 2007). Application of OTC has been shown to activate existing soil pollutants such as heavy metals in contaminated soils in previous studies in our laboratory, indicating an elevated environmental risk in soils with multiple contaminants (Chen et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Toxicity of OTC to Ipomoea aquatica Forsk. and to microorganisms in a long-term sewage-irrigated farmland soil

Chen

et al. 2016

Environ Sci Pollut Res

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Water spinach (Ipomoea aquatic Forsk.) was selected to investigate the effects of oxytetracycline (OTC) on the toxicity of soil contaminated by long-term sewage irrigation. After acute toxicity test in petri dish at nine different OTC-spiked levels for 48 h, the germination rate was found to be generally inhibited in all treatments treated with OTC but the root elongation and activities of several antioxidant enzymes, superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) were either forward or backward stimulated to varying extent. During a 60-day sub-chronic toxicity test by means of a pot experiment, activities of SOD, POD and CAT in both the leaf and root tissue at 25 mg OTC per kg soil (dry weight) and in root tissue at 1 mg OTC per kg soil (dry weight) were significantly different than those in other treatments, which also indicated the higher sensitivity of the root. The foliar photosynthetic rate, stomatal conductance and transpiration rate were all gradually inhibited in spite of elevated water use efficiency under the pressure of the different OTC concentrations, which were highly significant different at 10 mg OTC per kg soil (dry weight). Indices of soil microbial diversity at 4 mg OTC kg −1 soil were significantly different from those of the control, indicating the potential adverse effects of OTC to soil microorganisms. The results suggest that the introduction of OTC could damage both plants and soil microorganisms, and during sub-chronic incubation, the sensitivity of different indices generally followed the order of root tissue antioxidant enzyme activities, soil microbial diversity indices, leaf photosynthesis-related index and leaf tissue enzyme antioxidant activities. In addition, the application of livestock and poultry manure containing pollutants like OTC in farmland soil, especially if the soil has been contaminated before, should be taken more seriously in the context of the current pursuit of increased agricultural production.

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Uptake of oxytetracycline and its phytotoxicity to alfalfa (Medicago sativa L.)

Cited by 179 publications

References 36 publications

Mechanistic studies of perfluorooctane sulfonate, perfluorooctanoic acid uptake by maize (Zea mays L. cv. TY2)

Mechanistic studies of perfluorooctane sulfonate, perfluorooctanoic acid uptake by maize (Zea mays L. cv. TY2)

Concomitant uptake of antimicrobials and Salmonella in soil and into lettuce following wastewater irrigation

Toxicity of OTC to Ipomoea aquatica Forsk. and to microorganisms in a long-term sewage-irrigated farmland soil

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