Toxicity of OTC to Ipomoea aquatica Forsk. and to microorganisms in a long-term sewage-irrigated farmland soil

Abstract

Water spinach (Ipomoea aquatic Forsk.) was selected to investigate the effects of oxytetracycline (OTC) on the toxicity of soil contaminated by long-term sewage irrigation. After acute toxicity test in petri dish at nine different OTC-spiked levels for 48 h, the germination rate was found to be generally inhibited in all treatments treated with OTC but the root elongation and activities of several antioxidant enzymes, superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) were either forward or backward stimulated to varying extent. During a 60-day sub-chronic toxicity test by means of a pot experiment, activities of SOD, POD and CAT in both the leaf and root tissue at 25 mg OTC per kg soil (dry weight) and in root tissue at 1 mg OTC per kg soil (dry weight) were significantly different than those in other treatments, which also indicated the higher sensitivity of the root. The foliar photosynthetic rate, stomatal conductance and transpiration rate were all gradually inhibited in spite of elevated water use efficiency under the pressure of the different OTC concentrations, which were highly significant different at 10 mg OTC per kg soil (dry weight). Indices of soil microbial diversity at 4 mg OTC kg −1 soil were significantly different from those of the control, indicating the potential adverse effects of OTC to soil microorganisms. The results suggest that the introduction of OTC could damage both plants and soil microorganisms, and during sub-chronic incubation, the sensitivity of different indices generally followed the order of root tissue antioxidant enzyme activities, soil microbial diversity indices, leaf photosynthesis-related index and leaf tissue enzyme antioxidant activities. In addition, the application of livestock and poultry manure containing pollutants like OTC in farmland soil, especially if the soil has been contaminated before, should be taken more seriously in the context of the current pursuit of increased agricultural production.

Low-cost Posidonia oceanica bio-adsorbent for efficient removal of antibiotic oxytetracycline from water

“…Oxytetracycline (OTC) is an antibiotic applied for the treatment of bacterial infections in humans and animals (Ma et al 2016). Curative and preventive treatments of bacterial infections improve the production of farm animals; this is the reasons why OTC is widely used in animal husbandry such as aquaculture and livestock (Lu et al 2021;Qin et al 2022).…”

Application of catastrophe theory in comprehensive ecological security assessment of plastic greenhouse soil contaminated by phthalate esters

“…Tenfold serial dilutions were made, and1000-fold soil dilution solution was used for injection into the wells of the Biolog Eco plates. Plates were incubated at 25°C for 7 days, and color development was measured daily as difference in absorbance (A) at 590 nm using a μQuant microplate spectrophotometer (BioTek, Winooski, VT) before the data were calculated using Gen5v1.06 software [ 32 ].…”

Effects of Chlortetracycline on the Growth of Eggplant and Associated Rhizosphere Bacterial Communities

“…In addition to destroying the microbial community in the soil environment, TCs pollution would also be absorbed and accumulated by edible plants and even affect the growth of vegetables, posing a threat to global food security and soil ecosystem function, which has attracted growing concerns [19,20]. It has been reported that TCs can prevent the growth of crops and vegetables, such as wheat, ginger, and Ipomoea aquatica Forsk [2,19,21]. The influence of TCs on edible plants is closely related to the frequent use of livestock and poultry manure.…”