Uptake of calcium and silicon released from calcium silicate–based endodontic materials into root canal dentine

Han, Le; Okiji, Takashi

doi:10.1111/j.1365-2591.2011.01924.x

Cited by 314 publications

(312 citation statements)

References 37 publications

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“…[13,25] It has been speculated that facilitating the formation of an interfacial layer between MTA and dentin would result in a favourable sealing ability since it would enhance the adhesion and the DR of MTA. [14,22,25] In the present study, adhesion of cement to CH-treated dentin was greater than the adhesion of cement to TAP-treated dentin. Shokouhinejad et al [21] suggested the application of non-setting CH to neutralize the pH and to increase the DR of MTA prior to the placement of MTA in a low-pH environment.…”

Section: Resultsmentioning

confidence: 69%

“…[15,17,18] Both materials were reported to form an interfacial layer and a tag-like mineral structure extending to the dentinal tubules. [13,14,16,25] However, some differences in interfacial layers formed by MTA and BD were reported. [14,16] Han and Okiji [14] reported that BD released more Ca CC ions and formed more calcium phosphate precipitates and thicker Ca-and Si-rich dentin areas than MTA.…”

Section: Resultsmentioning

confidence: 99%

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Effect of medicaments used in endodontic regeneration technique on push-out bond strength of MTA and Biodentine

Türk

Fidler

2015

Biotechnology & Biotechnological Equipment

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The aim of the present study was to evaluate the effect of the intracanal medicaments used in regenerative endodontic treatment on push-out bond strength (PBS) of mineral trioxide aggregate (MTA) and Biodentine (BD). The root canals of 102 maxillary incisors were enlarged to simulate immature roots and were randomly divided into three groups (n D 34): a control group (no intracanal medicament) and two test groups, subjected to calcium hydroxide (CH) or triple antibiotic paste (TAP) medication for two weeks. After the medication removal, each group was divided in two subgroups and the coronal portion of each canal was filled with MTA or BD. After one week of storage, the coronal region of each root was horizontally sectioned and push-out test was performed. Data were analyzed by two-way analysis of variance and the Tukey post hoc test (P D 0.05). PBS values were significantly affected by the type of material (P < 0.001) and the type of medication used (P D 0.049), but no interaction was found (P D 0.97). The BD group showed significantly higher push-out resistance values than those of the MTA group (P < 0.001). TAP showed the lowest PBS values, which were significantly lower than those of the control group (P D 0.043) but not than those of CH (P D 0.229). After a two-week application period, TAP seemed to decrease the PBS of both cements, while CH did not. BD appeared to have higher PBS compared to MTA, regardless of medication.

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Section: Resultsmentioning

confidence: 69%

Section: Resultsmentioning

confidence: 99%

Effect of medicaments used in endodontic regeneration technique on push-out bond strength of MTA and Biodentine

Türk

Fidler

2015

Biotechnology & Biotechnological Equipment

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“…3 Biodentine was described as a bioactive dentin substitute with apatite formation after immersion in phosphate solution, 4 as the elemental uptake of Ca and Si into root canal dentin was found to be more prominent for Biodentine than for MTA. 5 Even though tricalcium silicate appears to be a common component of both MTA and Biodentine, X-ray diffractometry of unhydrated cements revealed that Biodentine consists of a triclinic form of tricalcium silicate, while MTA consists of the monoclinic form. 6 In comparison to other similar cements, Biodentine contains 30%-50% alumina (while Portland has less than 5%), 7 which produces an acid-resistant feature that may be useful in infection-laden sites of the human body, where the pH value drops significantly.…”

Section: Introductionmentioning

confidence: 99%

BiodentineTM is cytocompatible with human primary osteoblasts

et al. 2017

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Calcium silicate-based materials have been widely studied due to their resemblance to, and similar applicability of, mineral trioxide aggregate (MTA). Among these, Biodentine™ (BD) was specifically designed as a "dentin replacement" material for applications such as root perforations, apexification, treatment of resorptive lesions, and as a retrograde filling material. The present study aimed to assess the in vitro response of human primary osteoblasts to BD using MTA Angelus TM as a reference material, by simultaneously analyzing three different cell viability parameters, namely mitochondrial activity, membrane integrity, and cell density. BD and MTA extracts were prepared by incubation on culture media for 24 h or 42 days after mixing. Primary human osteoblasts were exposed to extracts for 24 h, at 37 o C with 5% CO 2 , and cell viability was evaluated by the XTT, NRU, and CVDE assays. Both materials induced cell viability levels higher than 70% when extracted for 24 h. However, when cells were exposed to extracts with increased conditioning times, MTA presented significant cytotoxic effects (p < 0.05) in comparison to the control and MTA at 24 h. After 42 days, the XTT assay identified a significant reduction in cell viability by BD when compared to the control (p<0.05), despite the fact that levels above the 70% viability cutoff were attained for biocompatible materials. It can be concluded that BD is cytocompatible with human primary osteoblasts, indicating its adequacy in direct contact with bone tissues.

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“…Therefore, Ca 3 SiO 5 is considered to be a good substitute for dentin in restorations 2,4 and is more resilient to shear force than other cements 10 . In another study, this material was shown to induce an inflammatory response upon initial contact with tissue, but later demonstrated biocompatible acceptance after 2 weeks 8 .…”

Section: Introductionmentioning

confidence: 99%

“…The product consists of a powder (tricalcium silicate, calcium carbonate and zirconium oxide as a radiopacifier) and a liquid (containing calcium chloride and a water-soluble polymer) that are mixed together to form the cement 3,4 .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the genotoxicity and mutagenicity of Ca3SiO5-based cement

et al. 2016

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Ca 3 SiO 5 is new cement based on the composition of Portland that has been developed to have superior physicochemical and biological properties. In a clinical evaluation, the cement did not appear to have cytotoxic properties and allowed for the proliferation of pulp cells and gingival fibroblasts. However, no previous studies have evaluated the genotoxicity or the mutagenicity of Ca 3 SiO 5 in vivo. Therefore, the goal of this study is to evaluate the genotoxic and mutagenic potential of Ca 3 SiO 5 -based cement in vivo. Twenty-four male Wistar rats were divided into 3 groups (n = 8). Group A rats received subcutaneous implantation of Ca 3 SiO 5 in the dorsum. Group B rats received a single dose of cyclophosphamide (positive control). Group C rats received subcutaneous implantation of empty tubes in the dorsum (negative control). After 24 hours, all animals were euthanized and the bone marrow of the femurs was collected for use in the comet assay and the micronucleus test. The comet assay revealed that the Ca 3 SiO 5 group had a tail intensity of 23.57 ± 7.70%, the cyclophosphamide group had a tail intensity of 27.43 ± 7.40%, and the negative control group had a tail intensity of 24.75 ± 5.55%. The average number of micronuclei was 6.25 (standard deviation, SD = 3.53) in the Ca 3 SiO 5 group, 9.75 (SD = 2.49) in the cyclophosphamide group, and 0.75 (SD = 1.03) in the negative control group. There was an increase in the micronuclei frequency in the Ca 3 SiO 5 group compared to that of the negative control group (p < 0.05). Our data showed that exposure to the Ca 3 SiO 5 -based cement resulted in an increase in the frequency of micronuclei, but no genotoxicity was detected according to the comet assay.

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Uptake of calcium and silicon released from calcium silicate–based endodontic materials into root canal dentine

Abstract: Both Biodentine and MTA caused the uptake of Ca and Si in the adjacent root canal dentine in the presence of PBS. The dentine element uptake was more prominent for Biodentine than MTA.

Cited by 314 publications

References 37 publications

Effect of medicaments used in endodontic regeneration technique on push-out bond strength of MTA and Biodentine

Effect of medicaments used in endodontic regeneration technique on push-out bond strength of MTA and Biodentine

BiodentineTM is cytocompatible with human primary osteoblasts

Evaluation of the genotoxicity and mutagenicity of Ca3SiO5-based cement

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