Upregulated effects of miR-7 in methicillin-resistant Staphylococcus aureus

Abstract

The aim of the study was to investigate the characteristic function of the upregulated effects of miR-7 in methicillin-resistant Staphylococcus aureus (MRSA). After separating the MRSA in clinic, the expression of miR-7 mRNA was tested by reverse transcription polymerase chain reaction. The overexpression, inhibition of miR-7, and control group were established by plasmid in vitro. Following transfection of the bacterial strain, the effect of β-lactam antibiotics in minimum inhibitory concentration (MIC) was observed using the microporous dilution method, and antibacterial effects in vitro were observed using the dynamic growth curve method. The expression of miR-7 in sensitive MRSA was upregulated distinctly, with significant difference (P<0.05). MIC and the number of bacteria in the miR-7 overexpression group significantly increased while the inhibition group decreased prominently, with significant difference (P<0.05). The control and null plasmid groups revealed no significant difference. In conclusion, miR-7 upregulated the antimicrobial activity of MRSA, and the intervention of its expression may become a possible antibacterial target.

Staphylococcus aureus colonisation in patients from a primary regional hospital

“…These strains are designated as methicillin-oxacillin resistant SA (MORSA) and are associated with treatment failure. From these reasons, it is clear that there is a need to monitor the incidence and antibiotic resistance of MRSA strains on a regular basis ( 8 ). There is also a need for the discovery of novel molecules that may have antibacterial activity against SA strains.…”

Screening candidate microR-15a- IRAK2 regulatory pairs for predicting the response to Staphylococcus aureus-induced mastitis in dairy cows

“…The biological analysis of miRNAs and their target genes can provide a good way for us to study the immunoregulatory process of mastitis caused by the infection of mammary tissue by S. aureus (Zhang et al ., 2016). The comparison of miRNA and mRNA expression profiles in S. aureus -inoculated vs. control tissue allowed us to identify key miRNAs and mRNAs pairs (Ramirez et al ., 2018) associated with development of mastitis in dairy cows.…”