UPLC-TOF-MS/MS metabolomics analysis of zebrafish metabolism by spirotetramat

Zhang, Jie; Qian, Le; Wang, Chen; Teng, Miaomiao; Duan, Manman; Chen, Xiangguang; Li, Xuefeng; Wang, Chengju

doi:10.1016/j.envpol.2020.115310

Cited by 14 publications

(9 citation statements)

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“…For example, the Fish Embryo Acute Toxicity (FET) Test (TG 236) outlines an acute embryonic exposure design from 0–96 hpf, which covers the organogenesis period [ 17 ]. For zebrafish metabolomics studies, short-term exposure effects could be assessed in terms of hours (e.g., 2.5–4 h; as described in, e.g., TG 236 in embryos and TG 203 in adult fish) or medium- to long-term exposures in the larval period (e.g., 3–7 days) [ 15 , 18 , 19 , 20 , 21 , 22 , 23 , 24 ]. For chronic exposure, test guidelines are available for larval fish up to 30 days (e.g., TG 210) and for adult fish up to 20 or 60 days (e.g., TGs 229 and 234), when individually dissected fish tissues (e.g., liver, brain, intestinal samples, gonads) or blood samples could be analyzed [ 25 , 26 , 27 ].…”

Section: Experimental Designmentioning

confidence: 99%

“…As a result of the low sample volume, embryos and larvae are usually pooled by treatment (e.g., control vs. exposed) for metabolomics analysis. Several studies reported the number of pooled individuals per sample group (e.g., 30–400) instead of weight [ 16 , 20 , 21 , 22 , 45 , 46 ]. Alternatively, Bai et al performed metabolomics analysis in 168 hpf larvae using the weight of 25 mg per pooled sample group [ 47 ].…”

Section: Experimental Designmentioning

confidence: 99%

“…Homogenization is a critical step for tissue samples [ 56 ]. Water and/or organic solvents are usually added to zebrafish samples and homogenization is usually performed using a bead beater with zirconium oxide beads (bead size 0.5 mm) for a few minutes (e.g., 4–6 min) [ 20 , 57 , 58 ]. For tissue samples, two beat cycles of 40 s at 6500 Hz followed by cooling in ice after each cycle can be sufficient to homogenize the samples [ 56 ].…”

Section: Experimental Designmentioning

confidence: 99%

“…For zebrafish samples, most studies do not describe in detail these latter parameters and their influence on the metabolite stability and sample homogenization. In addition to bead homogenization, the sample can also be kept in an ultrasound bath for 10–15 min [ 15 , 20 ]. This latter step should be carefully evaluated regarding the time, temperature, and frequency applied, since it can cause metabolite degradation [ 59 ].…”

Section: Experimental Designmentioning

confidence: 99%

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Mass Spectrometry-Based Zebrafish Toxicometabolomics: A Review of Analytical and Data Quality Challenges

et al. 2021

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Metabolomics has achieved great progress over the last 20 years, and it is currently considered a mature research field. As a result, the number of applications in toxicology, biomarker, and drug discovery has also increased. Toxicometabolomics has emerged as a powerful strategy to provide complementary information to study molecular-level toxic effects, which can be combined with a wide range of toxicological assessments and models. The zebrafish model has gained importance in recent decades as a bridging tool between in vitro assays and mammalian in vivo studies in the field of toxicology. Furthermore, as this vertebrate model is a low-cost system and features highly conserved metabolic pathways found in humans and mammalian models, it is a promising tool for toxicometabolomics. This short review aims to introduce zebrafish researchers interested in understanding the effects of chemical exposure using metabolomics to the challenges and possibilities of the field, with a special focus on toxicometabolomics-based mass spectrometry. The overall goal is to provide insights into analytical strategies to generate and identify high-quality metabolomic experiments focusing on quality management systems (QMS) and the importance of data reporting and sharing.

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Section: Experimental Designmentioning

confidence: 99%

Section: Experimental Designmentioning

confidence: 99%

Section: Experimental Designmentioning

confidence: 99%

Section: Experimental Designmentioning

confidence: 99%

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Mass Spectrometry-Based Zebrafish Toxicometabolomics: A Review of Analytical and Data Quality Challenges

et al. 2021

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“…This compound also causes biochemical, histopathological, and physiological changes in the ovaries of adult zebrafish (Danio rerio) (Wu et al, 2018). In zebrafish embryos, it is a teratogen agent (Zhang et al, 2019), affects lipid metabolism and causes mitochondrial lesions (Zhang et al, 2020). Spirodiclofen is considered to be a risk factor for adrenal cortical vacillation, atrophy of male reproductive tract Leydig cell hyperplasia and uterine adenocarcinoma in rats (Yoshida et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Movento® 240SC (Spirotetramat) and Envidor® 240SC (Spirodiclofen) keto-enol insecticides induce DNA damage in <i>Drosophila melanogaster</i> ovaries

González-Marín

Calderón-Segura

Pérez

et al. 2021

Fundam. Toxicol. Sci.

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Movento® 240SC and Envidor® 240SC are new insecticide derivatives of tetramic acid belonging to a keto-enol pesticide family. However, few studies have reported genotoxic effects in nontarget organisms. In the present study, the genotoxic effects of Movento® 240SC and Envidor® 240SC on Drosophila melanogaster ovaries were analyzed using the alkaline comet assay. Simultaneously, we determined the LD 50 for both insecticides. Virgin females were exposed to food at three sublethal concentrations (11.2, 22.4, 37.3 mg/L) of Movento® 240SC and (12.3, 24.6, 41.1 mg/L) of Envidor® 240SC for 72 hr. As a negative control group, females were exposed to food without insecticides, and as a positive control group, females were exposed to 17.5 mg/L bleomycin under the same experimental conditions. We analyzed three genotoxic parameters, tail length, tail moment, and tail intensity, in ovarian cells. The results showed that 11.2 mg/L Movento® 240SC insecticide significantly increased the tail intensity mean in ovarian cells compared with the negative control. However, 22.4 and 37.3 mg/L Movento® 240SC significantly increased tail length and tail moment means compared with the negative control. Envidor® 240SC insecticide at 12.3, 24.6, 41.1 mg/L significantly increased the three genotoxic parameters in ovarian cells compared with the negative control. The LD 50 values of Movento® 240SC and Envidor® 240SC insecticides were 79.1 mg/L and 78.0 mg/L, respectively. The genotoxic response of the two keto-enol pesticides was dependent on the concentration of each pesticide. The results demonstrated that Movento® 240SC and Envidor® 240SC keto-enol insecticides are genotoxic agents in D. melanogaster ovaries.

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Insight into the differences in the toxicity mechanisms of dinotefuran enantiomers in zebrafish by UPLC-Q/TOF–MS

Zhou

Yang

Ming

et al. 2022

Environ Sci Pollut Res

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UPLC-TOF-MS/MS metabolomics analysis of zebrafish metabolism by spirotetramat

Cited by 14 publications

References 54 publications

Mass Spectrometry-Based Zebrafish Toxicometabolomics: A Review of Analytical and Data Quality Challenges

Mass Spectrometry-Based Zebrafish Toxicometabolomics: A Review of Analytical and Data Quality Challenges

Movento® 240SC (Spirotetramat) and Envidor® 240SC (Spirodiclofen) keto-enol insecticides induce DNA damage in <i>Drosophila melanogaster</i> ovaries

Insight into the differences in the toxicity mechanisms of dinotefuran enantiomers in zebrafish by UPLC-Q/TOF–MS

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