Unveiling the potentials of biocompatible silver nanoparticles on human lung carcinoma A549 cells and Helicobacter pylori

Abstract

Silver nanoparticles (AgNPs) are gaining importance in health and environment. This study synthesized AgNPs using the bark extract of a plant, Toxicodendron vernicifluum (Tv) as confirmed by a absorption peak at 420 nm corresponding to the Plasmon resonance of AgNPs. The AgNPs were spherical, oval-shaped with size range of 2–40 nm as evident by field emission transmission electron microscopy (FE-TEM) and particle size analysis (PSA). The particles formed were crystalline by the presence of (111), (220) and (200) planes, as revealed by X ray diffraction (XRD) and energy dispersive spectroscopy (EDS). The presence of amine, amide, phenolic, and alcoholic aromatics derived from Tv extract was found to be capping and or reducing agents as evident by Fourier-transform infrared spectroscopy (FTIR) spectra. The Tv-AgNPs were observed to be biocompatible to chick embryonic and NIH3T3 cells at various concentrations. Interestingly, Tv-AgNPs at the concentration of 320 µg. mL −1 induced 82.5% of cell death in human lung cancer, A549 cells and further 95% of cell death with annexin V FITC/PI based apoptosis. The Tv-AgNPs selectively targeted and damaged the cancer cells through ROS generation. The Tv-AgNPs displayed minimal inhibitory concentration (MIC) of 8.12 µg.mL −1 and 18.14 µg.mL −1 against STEC and H. pylori respectively. This multi-potent property of Tv-AgNPs was due to shape and size specific property that facilitated easy penetration into the bacterial and cancer cells for targeted therapy.

Antimicrobial and Antibiofilm Activities of New Synthesized Silver Ultra-NanoClusters (SUNCs) Against Helicobacter pylori

“…The antimicrobial effect of different Ag + ion formulations against H. pylori has already been demonstrated in vivo and in vitro, but few studies on the efficacy of AgNPs are present in the literature do date. Very recently, a study about the effect of biocompatible AgNPs against H. pylori MH179988 has been published (Saravanakumar et al, 2019). The authors reported a MIC of 18.14 mg/L, which is 8 to 10 times higher than that found in the present study, highlighting the excellent bactericidal efficacy of SUNCs.…”

“…More in detail, AgNPs showed to be effective against H. pylori strains at very low concentrations (Amin et al, 2012(Amin et al, , 2014Gurunathan et al, 2015;Saravanakumar et al, 2019). They have also been tested on a large number of continuous and immortalized human cell lines in order to define their cytotoxic effect.…”

Unveiling the cytotoxicity of phytosynthesised silver nanoparticles using Tinospora cordifolia leaves against human lung adenocarcinoma A549 cell line

“…6 b ). Furthermore, it is stated that AgNPs initiate the cell apoptosis in various cancer cell lines such as human lung carcinoma A549, HepG2, human breast cancer cell MCF‐7, HCT116 [51].…”

A Review on Synthesis, Applications, Toxicity, Risk Assessment and Limitations of Plant Extracts Synthesized Silver Nanoparticles

“…The primary requirement for the in vivo applications is the biocompatibility of AgNPs with the cells. AgNPs synthesized using Toxicodendron vernicifluum aqueous bark extract were observed to be biocompatible to mouse embryo fibroblast cell line (NIH3T3 cells) at various concentrations [139]. Althaea officinalis leaf extract synthesized AgNPs were tested for genotoxicity evaluation in vivo on Zebra-fish (Danio rerio).…”