Untersuchungen zur Eiweißbindung von Arzneistoffen durch kontinuierliche Ultrafiltration, 1. Mitt. Eiweißbindung von Phenprocoumon

Rehse, Klaus; Rothe, Madeleine; Maurer, Rainer; Kinawi, Abdelwahab

doi:10.1002/ardp.19813141011

Cited by 14 publications

(1 citation statement)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Each of the above‐mentioned biologically active molecules is characterized by its specific physicochemical properties and behavior that change after the formation of the complex with the corresponding interacting partner. Based on these alterations, many different approaches to studying the interactions have been used–equilibrium dialysis , nuclear magnetic resonance , circular dichroism , fluorescence‐based techniques , isothermal titration calorimetry , continuous ultrafiltration , surface plasmon resonance (SPR) , Fourier transform infrared spectroscopy , MS , Raman spectroscopy , molecular modeling , HPLC , and thermophoresis , including CE as well. Besides the substantial instrumental development, the necessity of measured data quality as high as possible is essential requirement of these studies.…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis‐based approaches for the study of affinity interactions combined with various sensitive and nontraditional detection techniques

2019

View full text Add to dashboard Cite

Nearly all processes in living organisms are controlled and regulated by the synergy of many biomolecule interactions involving proteins, peptides, nucleic acids, nucleotides, saccharides, and small molecular weight ligands. There is growing interest in understanding them, not only for the purposes of interactomics as an essential part of system biology, but also in their further elucidation in disease pathology, diagnostics, and treatment. The necessity of detailed investigation of these interactions leads to the requirement of laboratory methods characterized by high efficiency and sensitivity. As a result, many instrumental approaches differing in their fundamental principles have been developed, including those based on capillary electrophoresis. Although capillary electrophoresis offers numerous advantages for such studies, it still has one serious limitation, its poor concentration sensitivity with the most commonly used detection method–ultraviolet‐visible spectrometry. However, coupling capillary electrophoresis with a more sensitive detector fulfils the above‐mentioned requirement. In this review, capillary electrophoresis combined with fluorescence, mass spectrometry, and several nontraditional detection techniques in affinity interaction studies are summarized and discussed, together with the possibility of conducting these measurements in microchip format.

show abstract

Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis‐based approaches for the study of affinity interactions combined with various sensitive and nontraditional detection techniques

2019

View full text Add to dashboard Cite

show abstract

Untersuchungen zur Eiweißbindung durch kontinuierliche Ultrafiltration, 2. Mitt. Eiweißbindung von Warfarin

Rehse¹,

Rothe²,

Kinawi³

1981

Archiv der Pharmazie

View full text Add to dashboard Cite

Die Eiweißbindung von Warfarin an menschliches Serumalbumin wurde durch kontinuierliche Ultrafiltration in den Konzentrationsbereichen (ctotal) 0.6–4.2 μg · ml−1 (Fluoreszenzdetektion) und 100–400 μg · ml−1 (UV‐Detektion) gemessen. Der freie Anteil α ist bis zu einer Gesamtkonzentration von 100 μg · ml−1 nahezu konstant und beträgt 0.5%. Auch die übrigen Parameter stimmen mit den Werten aus anderen Methoden recht gut überein. Der Vergleich mit der Eiweißbindung von Phenprocoumon zeigt im therapeutischen Konzentrationsbereich keine signifikanten Unterschiede.

show abstract

Untersuchungen zur Eiweißbindung von Arzneistoffen durch kontinuierliche Ultrafiltration, 3. Mitt.⁵⁾ Eiweißbindung von blutgerinnungshemmenden Tetronsäuren

Rehse

Rothe

Kühn

1982

Archiv der Pharmazie

View full text Add to dashboard Cite

show abstract

Untersuchungen zur Eiweißbindung von Arzneistoffen durch kontinuierliche Ultrafiltration, 1. Mitt. Eiweißbindung von Phenprocoumon

Cited by 14 publications

References 7 publications

Capillary electrophoresis‐based approaches for the study of affinity interactions combined with various sensitive and nontraditional detection techniques

Capillary electrophoresis‐based approaches for the study of affinity interactions combined with various sensitive and nontraditional detection techniques

Untersuchungen zur Eiweißbindung durch kontinuierliche Ultrafiltration, 2. Mitt. Eiweißbindung von Warfarin

Untersuchungen zur Eiweißbindung von Arzneistoffen durch kontinuierliche Ultrafiltration, 3. Mitt.⁵⁾ Eiweißbindung von blutgerinnungshemmenden Tetronsäuren

Contact Info

Product

Resources

About

Untersuchungen zur Eiweißbindung von Arzneistoffen durch kontinuierliche Ultrafiltration, 1. Mitt. Eiweißbindung von Phenprocoumon

Cited by 14 publications

References 7 publications

Capillary electrophoresis‐based approaches for the study of affinity interactions combined with various sensitive and nontraditional detection techniques

Capillary electrophoresis‐based approaches for the study of affinity interactions combined with various sensitive and nontraditional detection techniques

Untersuchungen zur Eiweißbindung durch kontinuierliche Ultrafiltration, 2. Mitt. Eiweißbindung von Warfarin

Untersuchungen zur Eiweißbindung von Arzneistoffen durch kontinuierliche Ultrafiltration, 3. Mitt.5) Eiweißbindung von blutgerinnungshemmenden Tetronsäuren

Contact Info

Product

Resources

About

Untersuchungen zur Eiweißbindung von Arzneistoffen durch kontinuierliche Ultrafiltration, 3. Mitt.⁵⁾ Eiweißbindung von blutgerinnungshemmenden Tetronsäuren