Untersuchungen an Zwillingen vonBeta vulgaris L.

Fischer, Hans Eberhard

doi:10.1007/bf00713461

Cited by 14 publications

(2 citation statements)

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“…In this case, two, or rarely, more embryos develop. The "twin embryos" character is heritable and is quite well distributed among 4x genotypes and in 3x hybrids (Fischer, 1956). Their percentage can be reduced by separation on a gravity table, simultaneously with empty seeds.…”

Section: Methods Of Seed Productionmentioning

confidence: 99%

Root and Tuber Crops

Bradshaw¹

2010

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Section: Methods Of Seed Productionmentioning

confidence: 99%

Root and Tuber Crops

Bradshaw¹

2010

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“…The first haploid plant in B. vulgaris species was detected and described in the analysis of seed progeny of sugar beet obtained in experiments using colchicine treatment of shoots [16]. Later, haploids were found in the progeny of plants treated with polyploidizing agents: among twin seeds isolated from diploid or anisoploid varieties, among the progeny of diploid cytoplasmic plants with male sterility, among spontaneous twins and plantlets produced by irradiated pollen, and by distant crosses (Zimmermann (1953) [17], Fischer (1956) [18], Butterfass [19], Kruse (1961) [20], Dobretsova et al, (1965) [21] and Hammond (1966) [22]; Bosemark (1971) [23]). However, for a long time, haploid sugar beet was not used in breeding since traditional methods allowed selecting only a limited number of haploid sugar beet plants [24]; moreover, there were no protocols for multiplication and maintenance of haploid plants in in vitro culture, for transfer to the diploid level.…”

Section: Introductionmentioning

confidence: 99%

Production of Gynogenic Plants of Red Beet (Beta vulgaris L.) in Unpollinated Ovule Culture In Vitro

Zayachkovskaya

Домблидес

Zayachkovsky

et al. 2021

Plants

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The unique and balanced components of the biochemical composition, together with high antioxidant activity, make the red beet necessary a dietary vegetable crop, much contributing to healthy food ration. The application of the technology for producing gynogenic plants in vitro increases the genetic diversity and significantly reduces the period of time required to obtain the appropriate homozygous lines used to create the F1 hybrids that are demanded in the market. For induction of gynogenesis, we used IMB medium developed by us with the addition of 55 g/L sucrose, 3 g/L phytogel, 200 mg/L ampicillin, and 0.4 mg/L thidiazuron (TDZ) and cultured at 28 °C in the dark for 4–6 weeks. Shoot regeneration from embryoids and callus was performed on MS medium with 20 g/L sucrose, 3 g/L phytogel, 1 mg/L 6-benzylaminopurine (BAP), and 0.1 mg/L gibberellic acid (GA3). Immersion of the obtained microshoots with 5–7 well-developed leaves for 10–15 s into concentrated sterile indole-3-butyric acid (IBA) solution (50 mg/L) followed by their cultivation on solid medium ½ IMB with 2% sucrose and 3 g/L phytogel was the most efficient method for root formation. The addition of silver nitrate (22 mg/L) to the nutrient medium provoked an increase in the number of induced ovules up to nine per Petri dish (up to 25% of induced ovules). Gynogenic development was produced in six out of 11 genotypes studied, and the plants that were then acclimatized to ex vitro conditions were obtained in three genotypes (Nezhnost’, Dobrynya, b/a 128). The evaluation of ploidy of gynogenic plants that was carried out by flow cytometry and direct counting of chromosomes stained with propion-lacmoide revealed that all obtained gynogenic plants were haploids (2n = x = 9).

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Sugar Beet Breeding

McGrath

Panella

2018

Plant Breeding Reviews

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Untersuchungen an Zwillingen vonBeta vulgaris L.

Cited by 14 publications

References 26 publications

Root and Tuber Crops

Root and Tuber Crops

Production of Gynogenic Plants of Red Beet (Beta vulgaris L.) in Unpollinated Ovule Culture In Vitro

Sugar Beet Breeding

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