Untargeted Metabolomics Reveals a Complex Impact on Different Metabolic Pathways in Scallop Mimachlamys varia (Linnaeus, 1758) after Short-Term Exposure to Copper at Environmental Dose

Abstract

Ports are a good example of how coastal environments, gathering a set of diverse ecosystems, are subjected to pollution factors coming from human activities both on land and at sea. Among them, trace element as copper represents a major factor. Abundant in port ecosystem, copper is transported by runoff water and results from diverse port features (corrosion of structures, fuel, anti-fouling products, etc.). The variegated scallop Mimachlamys varia is common in the Atlantic port areas and is likely to be directly influenced by copper pollution, due to its sessile and filtering lifestyle. Thus, the aim of the present study is to investigate the disruption of the variegated scallop metabolism, under a short exposure (48 h) to a copper concentration frequently encountered in the waters of the largest marina in Europe (82 μg/L). For this, we chose a non-targeted metabolomic approach using ultra-high performance liquid chromatography coupled to high resolution mass spectrometry (UHPLC-HRMS), offering a high level of sensitivity and allowing the study without a priori of the entire metabolome. We described 28 metabolites clearly modulated by copper. They reflected the action of copper on several biological functions such as osmoregulation, oxidative stress, reproduction and energy metabolism.

Metabolomic analysis of honey bee (Apis mellifera L.) response to glyphosate exposure

“…Metabolomics is an analytical tool that can quickly and quantitatively measure the changes in a range of metabolites in response to an external stressor and provide an evaluation of the overall biological functioning of an individual at the molecular level. [21][22][23] In ecotoxicology, the metabolomics approach can help identify the unique metabolite profiles or 'fingerprints' in an organism after toxin exposure, which can serve as biomarkers for future exposure to the same compound. [24][25][26] The most established protocol in metabolomics is the metabolic profiling of biological fluids, such as plasma and urine, in mammalian systems since these fluids are easy to collect and store.…”

Hemolymph Metabolism Analysis of Honey Bee (Apis mellifera L.) Response to Different Bee Pollens

“…Metabolomics is an analytical tool that can be used to quickly and quantitatively measure the changes in a range of metabolites in response to an external stressor and has been shown to have some advantages over other postgenomic technologies [ 22 ]. Unlike the transcriptome or the proteome, which vary from species to species, the basic metabolic pathways and their metabolites are similar among different species, making metabolomic analysis much more universally applicable [ 23 ].…”