Hemolymph Metabolism Analysis of Honey Bee (Apis mellifera L.) Response to Different Bee Pollens

Chang, Hongcai; Ding, Guowei; Jia, Guanqing; Mao, Feng; Huang, Jiaxing

doi:10.3390/insects14010037

Cited by 3 publications

(3 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This result is consistent with the general statement that pollen rich in essential amino acids is more nutritious than other amino acids. Tryptophan plays an important role in the development, olfactory learning, and memory abilities of honey bee workers [ 76 ]. In addition, tryptophan can stimulate the development of the pharyngeal gland, which translates into greater production of royal jelly in nurses [ 76 ].…”

Section: The Key Role Of Protein and Amino Acids In The Metabolism Of...mentioning

confidence: 99%

“…Tryptophan plays an important role in the development, olfactory learning, and memory abilities of honey bee workers [ 76 ]. In addition, tryptophan can stimulate the development of the pharyngeal gland, which translates into greater production of royal jelly in nurses [ 76 ].…”

Section: The Key Role Of Protein and Amino Acids In The Metabolism Of...mentioning

confidence: 99%

“…Moreover, the periods of rapeseed, apricot and pear flowering overlap and bees are observed to have flower preferences. According to Chang et al [ 76 ], more frequent visits to rapeseed flowers, as opposed to pear flowers, result from the higher sugar content in the rapeseed nectar. In a laboratory experiment, bees fed a mono diet showed a significant preference for apricot pollen over pear pollen.…”

Section: The Phenomenon Of Hunger and Low Protein Diversity In A Bee ...mentioning

confidence: 99%

See 2 more Smart Citations

The Key Role of Amino Acids in Pollen Quality and Honey Bee Physiology—A Review

Bryś,

Strachecka

2024

Molecules

View full text Add to dashboard Cite

When studying honey bee nutrition, it is important to pay attention not only to the quantity but also to the quality of pollen for floral visitors. The recommended way to determine the value of pollen is to determine both the protein concentration and the amino acid composition in the insect’s hemolymph. In addition, the composition of pollen also includes lipids, sterols and biogenic elements such as carbon, nitrogen, etc. Very high protein concentration is observed in aloe pollen, averaging 51%. Plants with a high protein content, at the level of 27% in Europe, are rapeseed and phacelia. In turn, a plant that is poor in protein (at the level of 11%) is buckwheat. The aforementioned plants are sown over very large areas. Vast acreages in Central and Eastern Europe are occupied by pollen- and nectar-providing invasive plants, such as goldenrod. Therefore, bees are forced to use one food source—a mono diet—which results in their malnutrition. In the absence of natural pollen, beekeepers use other foods for bees; including soy protein, powdered milk, egg yolks, fish meal, etc. However, the colony is the strongest when bees are fed with pollen, as opposed to artificial protein diets. More research is needed on the relationship between bee pollen composition and nutrition, as measured by protein concentration and amino acid composition in apian hemolymph, colony strength, honey yield and good overwintering.

show abstract

Section: The Key Role Of Protein and Amino Acids In The Metabolism Of...mentioning

confidence: 99%

Section: The Key Role Of Protein and Amino Acids In The Metabolism Of...mentioning

confidence: 99%

Section: The Phenomenon Of Hunger and Low Protein Diversity In A Bee ...mentioning

confidence: 99%

See 1 more Smart Citation

The Key Role of Amino Acids in Pollen Quality and Honey Bee Physiology—A Review

Bryś,

Strachecka

2024

Molecules

View full text Add to dashboard Cite

show abstract

The effect of the method of preservation of bee honey on the development of the pharyngeal gland in honey bees Apis mellifera L

Kovalskyi,

Perig

2023

Tehnologìâ virobnictva ì pererobki produktìv tvarinnictva

View full text Add to dashboard Cite

Modern agricultural systems involve the use of large monocultures, which significantly limits the variety of flowers for bees. The article presents data on the biochemical composition of monofloral bee pollen and its influence on the development of pharyngeal glands in honey bees. The conducted studies give reason to believe that the protein content in bee pollen depends on the species origin and the preservation method. The studied samples for protein content show that its amount ranges from 11.9 to 24.9 %. The maximum amount of protein was found in fresh rapeseed and apple bee pollen. To study the influence of monofloral diets on the growth and development of the pharyngeal gland, 9 groups of analogous bee families were formed, 3 in each, which were fed three types of feed. The first type is fresh bee pollen from the following plants: apple tree, dandelion, winter rapeseed, buckwheat, clover, chestnut, raspberry. The second type is a similar bee pollen, only after a year of deep freezing storage. The ration of the third species consisted of the indicated bee pollen only in the process of harvesting it was dried. Along with this, the formed group of bee families was divided into two more subgroups. Some consumed mixed feed in equal proportions, others did not have any protein feed. The duration of the experiment was 15 days. On the 10th day, the development of acini of the pharyngeal gland was determined. The morphometry of the acini and the dynamics of vesicle filling indicate that the best indicators were found in the group of bees that consumed mixed bee pollen. A highly beneficial increase in the length and width of the acini of the pharyngeal gland was revealed by 36.4 and 34.7 % compared to bees that did not consume pollen at all. A group of bees that received fresh rapeseed and apple seed in their diet fully demonstrated their potential for royal jelly production. The worst development of the pharyngeal gland was found in the group of bees that consumed dried dandelion nectar. In this group of bees, the area of the acini ranged from 15.5 to 16.4 thousand μm2. The relationship between the protein content in the diet and the degree of development of the pharyngeal gland was revealed. Of the researched methods of its preservation, the conditions of deep freezing turned out to be the best. The use of experimental monofloral diets has a negative effect on the internal indicators of the body of honey bees. Key words: Apis mellifera L., pharyngeal gland, nutrition, protein, bee pollen, pollen storage.

show abstract

Revealing Changes in Ovarian and Hemolymphatic Metabolites Using Widely Targeted Metabolomics between Newly Emerged and Laying Queens of Honeybee (Apis mellifera)

Zhong,

Pan,

Wang

et al. 2024

Insects

View full text Add to dashboard Cite

The queen bee is a central and pivotal figure within the colony, serving as the sole fertile female responsible for its reproduction. The queen possesses an open circulatory system, with her ovaries immersed in hemolymph. A continuous and intricate transportation and interchange of substances exist between the ovaries and hemolymph of queen bees. To determine the characteristic metabolites in the hemolymph and ovary, as well as understand how their rapid metabolism contributes to the process of egg-laying by queens, we reared Apis mellifera queens from three different age groups: newly emerged queen (NEQ), newly laying queen (NLQ), and old laying queen (OLQ). Using widely targeted metabolomics, our study revealed that the laying queen (NLQ and OLQ) exhibited faster fatty acid metabolism, up-regulated expression of antioxidants, and significant depletion of amino acids compared to the NEQ. This study revealed that the levels of carnitine and antioxidants (GSH, 2-O-α-D-glucopyranosyl-L-ascorbic acid, L-ascorbic acid 2-phosphate, etc.) in the NLQ and OLQ were significantly higher compared to NEQ. However, most of the differentially expressed amino acids, such as L-tryptophan, L-tyrosine, L-aspartic acid, etc., detected in NLQ and OLQ were down-regulated compared to the NEQ. Following egg-laying, pathways in the queens change significantly, e.g., Tryptophan metabolism, Tyrosine metabolism, cAMP signaling pathway, etc. Our results suggest that carnitine and antioxidants work together to maintain the redox balance of the queen. Additionally, various amino acids are responsible for maintaining the queen’s egg production.

show abstract

Hemolymph Metabolism Analysis of Honey Bee (Apis mellifera L.) Response to Different Bee Pollens

Cited by 3 publications

References 50 publications

The Key Role of Amino Acids in Pollen Quality and Honey Bee Physiology—A Review

The Key Role of Amino Acids in Pollen Quality and Honey Bee Physiology—A Review

The effect of the method of preservation of bee honey on the development of the pharyngeal gland in honey bees Apis mellifera L

Revealing Changes in Ovarian and Hemolymphatic Metabolites Using Widely Targeted Metabolomics between Newly Emerged and Laying Queens of Honeybee (Apis mellifera)

Contact Info

Product

Resources

About