Undibacterium terreum sp. nov., isolated from permafrost soil

Abstract

The bacterial strain C3 T was isolated from permafrost soil in Beijicun, Mohe County, Heilongjiang province, China. Cells of strain C3 T were Gram-stain-negative rods, 0.3-0.4 mm in diameter and 1.0-2.6 mm in length. Strain C3 T was strictly aerobic. Growth occurred at 15-37 6C but not at 4 or 42 6C, at pH 5.0-9.0 (optimum pH 6.0-7.0) and in the presence of 0-8 g NaCl l "1 (optimum 0-1 g l "1). The analysis of 16S rRNA gene sequences indicated that strain C3 T was phylogenetically related to members of the genus Undibacterium, with similarities ranging from 94.7 to 96.5 %. Strain C3 T contained ubiquinone 8 as the major respiratory quinone. The major cellular fatty acids were summed feature 3 (C 16 : 1 v7c/C 16 : 1 v6c), C 17 : 0 cyclo, straight-chain C 16 : 0 , C 12 : 0 and C 10 : 0 , unsaturated C 18 : 1 v7c and hydroxylated fatty acids C 10 : 0 3-OH and C 12 : 0 2-OH. The polar lipids were mainly phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The polyamines were putrescine and 2-hydroxyputrescine. The DNA G+C content was 57.4 mol% (determined from T m). Based on these results, it is concluded that strain C3 T represents a novel species of the genus Undibacterium, for which the name Undibacterium terreum sp. nov. is proposed, with C3 T (5CGMCC 1.10998 T 5NBRC 108789 T) representing the type strain.

Undibacterium macrobrachii sp. nov., isolated from a freshwater shrimp culture pond

“…T had Q-8 as the major respiratory quinone, which is the same as that of all other species of the genus Undibacterium (Kämpfer et al, 2007;Eder et al, 2011;Liu et al, 2013;Kim et al, 2014).…”

“…Putrescine and 2-hydroxyputrescine were the major polyamines of strain CMJ-9 T , in line with all other species of the genus Undibacterium for which the polyamine composition has been analysed (Kämpfer et al, 2007;Eder et al, 2011;Liu et al, 2013).…”

“…At the time of writing, the genus Undibacterium comprised six species with validly published names: Undibacterium pigrum (type species; Kämpfer et al, 2007), U. parvum (Kämpfer et al, 2007;Eder et al, 2011), U. oligocarboniphilum (Eder et al, 2011), U. terreum (Liu et al, 2013), U. jejuense and U. seohonense (Kim et al, 2014). Cells are characteristically Gram-staining-negative, oxidase-positive, rod-shaped, motile by a single polar flagellum and chemoheterotrophic.…”

“…Cells are characteristically Gram-staining-negative, oxidase-positive, rod-shaped, motile by a single polar flagellum and chemoheterotrophic. Members of the genus possess Q-8 as the major respiratory quinone, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol as predominant polar lipids, putrescine and 2-hydroxyputrescine as predominant polyamines, C 16 : 0 , summed feature 3 (C 16 : 1 v7c/C 16 : 1 v6c) and C 18 : 1 v7c as predominant fatty acids, C 10 : 0 3-OH as the major hydroxy fatty acid and DNA G+C contents of 50.6-57.4 mol% (Kämpfer et al, 2007;Eder et al, 2011;Liu et al, 2013). The present study was carried out to clarify the taxonomic position of the Undibacterium-like bacterial strain CMJ-9…”

Undibacterium arcticum sp. nov., isolated from arctic alpine soil

“…At the time of writing, this genus comprised nine species with validly published names: Undibacterium pigrum (type species; K€ ampfer et al, 2007), U. parvum (K€ ampfer et al, 2007;Eder et al, 2011), U. oligocarboniphilum (Eder et al, 2011), U. terreum (Liu et al, 2013), U. jejuense and U. seohonense (Kim et al, 2014), U. macrobrachii , U. squillarum and U. aquatile (Du et al, 2015). Cells are characteristically Gram-stain-negative, oxidase-positive, rod-shaped, motile by a single polar flagellum and chemoheterotrophic.…”