Uncultivable Bacteria: Implications and Recent Trends Towards Identification

Bhattacharya, Sanjay; Vijayalakshmi, Natesan; Sc, Parija

doi:10.1016/s0255-0857(21)03184-4

Cited by 9 publications

(2 citation statements)

References 14 publications

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“…For bacteriological examination, heart blood and faecal samples stored at 4C were processed on brain heart infusion agar, blood agar, MacConkey Agar and Eosin Methylene Blue Agar using a standard loop and incubated at 37C for 24-48 hours. Isolates were identified by colony morphology and gram staining (Bhattacharya, 2002). The antibiotic sensitivity test was carried out in Mueller Hinton agar using disc diffusion method as per standard procedures described by Bauer (Bauer et al, 1966).…”

Section: Methodsmentioning

confidence: 99%

Pathology of PMWS Associated with Classical Swine Fever Virus, Porcine Parvo Virus and Multidrug Resistant Enteric Escherichia coli Infections in an Organized Swine Farm, Rajasthan, India

Das

Sethi

John

et al. 2022

IJAR

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Background: Postweaning multisystemic wasting syndrome (PMWS) is caused by porcine circovirus type 2 (PCV2). It is a globally emerging clinical condition which affects nursing piglets and growing pigs. PCV2 requires various cofactors for fully development of PMWS. The present report describes the pathology of PMWS associated with classical swine fever virus (CSFV), porcine parvo virus (PPV) and multidrug resistant enteric Escherichia coli infections in an organized swine farm, Rajasthan. In India, this is the first report on pathology of PMWS associated with all the three viruses and multidrug resistant enteric colibacillosis. Methods: Detailed necropsy was conducted on pig carcass submitted to Post-mortem Facility, Division of Pathology, ICAR-Indian Veterinary Research Institute (IVRI), Izatnagar in the year 2017 from an organized swine farm, Rajasthan, India. Heart blood and diarrhoeic intestinal contents were collected aseptically and stored separately at 4°C for bacteriological examination and antibiotic sensitivity test. Tissue samples were collected in sterile container and stored at -40°C for DNA and RNA extraction and polymerase chain reaction (PCR) and also collected in 10% neutral buffered saline for histopathological processing. Result: The organized farm was having history of 33% (200/600) of mortality among 2 months to 6 months old piglets. Gross lesions were wasting, lymphadenopathy, non-collapsed lungs, hydropericardium, hepatomegaly, icterus, enteritis and pale kidneys, button shaped ulcers in the caecum and colon etc. Histopathologically, lymphoid depletion, reticuloendothelial cell proliferation in lymphoid tissue, fibrin thrombi in spleen, severe hepatitis, interstitial pneumonia, interstitial nephritis, severe necrosis in large intestine, meningitis, perivascular cuffing (PVC), gliosis, neuronal degeneration in cerebrum etc were observed. Molecular diagnosis and bacteriological examination confirmed presence of PCV2, PPV, CSFV and multidrug resistant Escherichia coli.

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Section: Methodsmentioning

confidence: 99%

Pathology of PMWS Associated with Classical Swine Fever Virus, Porcine Parvo Virus and Multidrug Resistant Enteric Escherichia coli Infections in an Organized Swine Farm, Rajasthan, India

Das

Sethi

John

et al. 2022

IJAR

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“…Different microorganisms grow in different environments and have variety of growth requirements; like nutrients, pH, osmotic conditions and temperature. The current limitations of cultivation of microbes in lab need to be addressed by formulation of newer media (Bhattacharya S, et al 2002) 8 .…”

Section: Introductionmentioning

confidence: 99%

Date Powder as Alternative Carbone Source in Optimizing the Cultivation of (Bacillus megaterium) in Submerged State Fermentation

Al-Taweil¹,

Aldawood²,

Alhamal

2021

J. Drug Delivery Ther.

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Problems statement: A study had shown a strain of Bacillus megaterium was isolated from the soil. Questions were whether date powder could be used as alternatives of carbon sources for Bacillus megaterium submerged state cultivation? Approach: To evaluate the effects of the using date powder; The fermentation was carried out in shake flasks using a complex medium consisting of (g/L) ammonium tartrate, 2.0; magnesium sulphateheptahydrate, 4.0; dipotassium phosphate, 14.0; calcium chloride, 0.2, NaHPO4, 4.0, yeast extract, 3.0, trace elements, 2.0ml and glucose 6.0(4.0, yeast extract, 3.0, trace elements, 2.0ml and glucose 6.0) were replaced by dates powder at three rates; 5,10 and15 g/L Results show that the rate of production of biomass during submerged fermentation by Bacillus megaterium at 30±1°C for 24 hours of incubation using date powder was maximizes between 18 and 24 hr of fermentation. The experimental runs and results for the Box– Behnken design. The 15 runs in a single block were used to study the effects using date powder in different rates on one response. Biomass concentration ranged from 6.5cfu /l to 12.9cfu /.comparing with 14.8 cfu/ g for the commercial growth media. The ANOVA tables give the statistical significance of the effects for biomass. These effects were dates powder rates, the optimized growth medium with pH 7, inoculums’ size 106, temp 24oC and moisture dates powder 15 g/L. The biomass concentrations obtained under the optimal conditions 12.9cfu/l. Key words: Date Powders, Fermentation, Bacillus megaterium, biomass, Submerged State

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