Ultraviolet photoacoustic microscopy with tissue clearing for high-contrast histological imaging

Li, Xiufeng; Kot, Jack C.K.; Tsang, Victor T. C.; Lo, Claudia T. K.; Huang, Bingxin; Tian, Ye; Wong, Ivy H. M.; Cheung, Helen H.Y.; Kang, Lei; Chang, Atta Cheuk Yan; Wong, Terence T. W.

doi:10.1016/j.pacs.2021.100313

Cited by 14 publications

(8 citation statements)

References 29 publications

(39 reference statements)

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“…Tissue clearing techniques have a wide range of applications in OR-PAM [30,31]. The presence of high refractive index substances, such as lipids and pigments, and low refractive index substances, such as water, in tissues results in strong light absorption and scattering.…”

Section: Introductionmentioning

confidence: 99%

High‐resolution in vivo imaging of human nailbed microvasculature by using photoacoustic microscopy

Zhao

Tao

et al. 2023

Journal of Biophotonics

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Microcirculation imaging has significantly clinical value in early diagnosis and curative effect judgment of various diseases. The most superficial layer of the nailbed is rich in capillaries, which is suitable as a window on the microcirculation. However, few techniques can noninvasively observe the blood supply distribution of the nailbed, especially for high-resolution imaging of capillaries. In this study, we adapted an opticalresolution photoacoustic microscopy (OR-PAM) to image the nailbed microvasculature. The imaging sensitivity was significantly improved by hydration pretreatment of the nail. In vitro phantom experiments demonstrate that the sensitivity was improved about 3.5 times after hydration. In vivo imaging experiments of the nailbed microvasculature were conducted to further examine the enhanced sensitivity and practicability of OR-PAM. Moreover, the quantitative analysis of capillary loops showed that OR-PAM can extract the detection indicators including vascular morphology, diameter, and length, which provides a basis for clinical microcirculation detection using OR-PAM.

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Section: Introductionmentioning

confidence: 99%

High‐resolution in vivo imaging of human nailbed microvasculature by using photoacoustic microscopy

Zhao

Tao

et al. 2023

Journal of Biophotonics

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“…Figure 2 shows the scanning trajectory of the UV-PAM system during imaging. Sub-image of each section (e.g., area of 5 mm x 30 µm) is first generated using a scattered interpolation algorithm, and then, a whole image (e.g., area of 5 mm x 5 mm) is obtained by stitching all the sub-images using custom image processing algorithm (see The lateral resolution of the present system is ~1.2 µm which has been experimentally measured in a recent publication 21 .…”

Section: Representative Resultsmentioning

confidence: 99%

High-Speed Ultraviolet Photoacoustic Microscopy for Histological Imaging with Virtual-Staining assisted by Deep Learning

Kang

et al. 2022

JoVE

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“…First, it is difficult to image thick and/or unprocessed biological samples with transmission-mode UV-PAM systems, which place the specimen between an optical objective lens and an ultrasound (US) transducer. [18,19] Therefore, the transmission-mode UV-PAM systems have been used only for the histopathology of thin specimens, which are impractical for clinical use. To successfully visualize histological morphology in thick samples for intraoperative FS diagnosis, reflection-mode UV-PAM systems, in which the optical excitation and US detection are located on the same side, have been developed.…”

Section: Introductionmentioning

confidence: 99%

An Ultraviolet‐Transparent Ultrasound Transducer Enables High‐Resolution Label‐Free Photoacoustic Histopathology

Kim,

Park,

Park

et al. 2023

Laser & Photonics Reviews

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Ultraviolet photoacoustic microscopy (UV‐PAM), based on the high intrinsic optical absorption of DNA/RNA, holds great promise for intraoperative label‐free histopathological imaging modalities. Although clinical histopathology requires high‐resolution images to observe individual cell structures, conventional UV‐PAM suffers from relatively low resolution compared to the clinical histological modalities. Notably, opto‐ultrasound beam combiners or ring‐shaped ultrasound transducers, which have been used as geometrically coaxial methods of conventional reflection‐mode UV‐PAMs, restrict the numerical apertures (NA) due to their long working distances and/or hollow structures. Here, an ultraviolet‐transparent ultrasound transducer (UV‐TUT) is demonstrated that enables high‐resolution reflection‐mode UV‐PAM by improving the NA (0.38) and consequent spatial resolution (0.47 ± 0.03 µm). The study has successfully demonstrated its superior performance with mouse brain tissue and performed photoacoustic histopathology with cancerous animal tissues. This work proposes the ultimate form of a label‐free reflection‐mode UV‐PAM system with the potential to boost the practical use of intraoperative photoacoustic histopathology.

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Ultraviolet photoacoustic microscopy with tissue clearing for high-contrast histological imaging

Cited by 14 publications

References 29 publications

High‐resolution in vivo imaging of human nailbed microvasculature by using photoacoustic microscopy

High‐resolution in vivo imaging of human nailbed microvasculature by using photoacoustic microscopy

High-Speed Ultraviolet Photoacoustic Microscopy for Histological Imaging with Virtual-Staining assisted by Deep Learning

An Ultraviolet‐Transparent Ultrasound Transducer Enables High‐Resolution Label‐Free Photoacoustic Histopathology

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