An Ultraviolet‐Transparent Ultrasound Transducer Enables High‐Resolution Label‐Free Photoacoustic Histopathology

Kim, Donggyu; Park, Eunwoo; Park, Jeongwoo; Perleberg, Bjarne; Jeon, Sora; Ahn, Joongho; Ha, Mingyu; Kim, Hyung Ham; Kim, Jin Young; Jung, Chan Kwon; Kim, Chulhong

doi:10.1002/lpor.202300652

Cited by 9 publications

(6 citation statements)

References 53 publications

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“…14 Addressing these challenges, we introduce an ultraviolet-transparent ultrasound transducer (UV-TUT), utilizing polyvinylidene fluoride (PVDF) film and silver nanowires (AgNWs). [15][16][17] With an optical transmittance higher than 60% at 266 nm, the UV-TUT surpasses the performance of a ring-shaped ultrasound transducer by a factor of four. The implementation of a UV-PAM system utilizing the UV-TUT produces impressive outcomes, achieving a high numerical aperture (NA) and lateral resolution.…”

Section: Introductionmentioning

confidence: 99%

High-resolution label-free ultraviolet photoacoustic microscopy via an ultraviolet-transparent ultrasound transducer

Kim,

Park,

Park

et al. 2024

Photons Plus Ultrasound: Imaging and Sensing 2024

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Ultraviolet photoacoustic microscopy (UV-PAM) has emerged as a promising medical imaging technique for alternative histopathology, relying on the inherent optical absorption of DNA/RNA. However, traditional UV-PAM faces resolution challenges compared to clinical histological methods, limiting the observation of cellular structures. This limitation stems from the constraints of conventional reflection-mode UV-PAM systems, utilizing opto-ultrasound beam combiners or ringshaped ultrasound transducers. These components impose constraints on numerical apertures (NA), thereby limiting spatial resolution. On the flip side, transmission-mode UV-PAM encounters difficulties in imaging thick specimens due to signal attenuation. In this study, we introduce an innovative solutionthe development of an ultraviolet-transparent ultrasound transducer (UV-TUT) overcoming these limitations and enabling high-resolution UV-PAM system. The UV-TUT significantly enhances both NA and lateral resolution, outperforming previous reflection-mode UV-PAM systems. With an impressive light transmission efficiency in the UV region and sensitivity four times greater than traditional ring-shaped ultrasound transducers, the UV-TUT lays the foundation for improved imaging capabilities. Leveraging the capabilities of the UV-TUT, we exploited a UV-PAM system, showcasing superior performance for imaging mouse brain tissue sections compared to conventional opto-ultrasound beam combiner-based UV-PAM. Furthermore, our application of photoacoustic histopathology on uterine cancer tissue sections demonstrated image quality comparable to microscopy images, providing valuable insights for accurate histopathological analysis. This work signifies a significant advancement in UV-PAM system, holding the promise to enhance the clinical utility of alternative histopathology with unprecedented resolution and imaging capabilities.

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Section: Introductionmentioning

confidence: 99%

High-resolution label-free ultraviolet photoacoustic microscopy via an ultraviolet-transparent ultrasound transducer

Kim,

Park,

Park

et al. 2024

Photons Plus Ultrasound: Imaging and Sensing 2024

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“…Photoacoustic tomography (PAT) uniquely provides multiparametric information using only one modality. PAT integrates optics and acoustics information, delivering structural, functional, and molecular details [3][4][5][6][7][8]. By the photoacoustic effect, pulse laser induces ultrasound signals from biological tissue, enabling high-resolution structure imaging of biomolecules such as hemoglobin [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Multiparametric tumor analysis through photoacoustic tomography system using a hemispherical transducer array

Kim,

Lee,

Choi

et al. 2024

Photons Plus Ultrasound: Imaging and Sensing 2024

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Photoacoustic tomography is an emerging technique in the field of biomedical imaging that combines the advantages of optics and acoustics. Unlike conventional imaging modalities, which are limited in their ability to provide diverse types of diagnostic information, PAT can offer structural, functional, and molecular biometric imaging using a single modality. This advantage can be utilized in that multiparametric information is required to analyze various aspects of tumor. In this study, we have developed a versatile PAT system for tumor imaging which can provide high-definition and high-speed images. Our system can identify tumor characteristics such as angiogenesis, pharmacokinetics, and physiological functions for both primary and metastatic tumors. During 14 days after tumor cell inoculations, we visualize the angiogenesis and increased tortuosity of blood vessels surrounding the tumor. Additionally, we quantify changes in the oxygen saturation within the entire tumor region, revealing the presence of hypoxia in the tumor core. These findings highlight the ability of our system to provide valuable functional information about tumor physiology. Moreover, our versatile PAT system allows us to observe the organ accumulation characteristics of different contrast agents. With its ability to generate high-quality structural, functional, and molecular photoacoustic images, our system holds promise for advancing the field of biomedical imaging.

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“… 11 – 20 This integration enhances the ability to capture subtle pathological signs and improves diagnostic accuracy. 21 – 23 As a hybrid optical and acoustical imaging modality, PA imaging provides unique photochemical contrast capabilities at previously uncharted depths within biological tissue, 24 – 29 surpassing the capabilities of other optical imaging methods. 30 – 37 It mirrors the US imaging process 38 – 42 but utilizes pulsed laser excitation to induce the PA effect in tissue chromophores (e.g., hemoglobin, melanin, lipid, and collagen).…”

Section: Introductionmentioning

confidence: 99%

Video-rate endocavity photoacoustic/harmonic ultrasound imaging with miniaturized light delivery

Oh,

Kim,

Sung

et al. 2024

J. Biomed. Opt.

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. Significance Endocavity ultrasound (US) imaging is a frequently employed diagnostic technique in gynecology and urology for the assessment of male and female genital diseases that present challenges for conventional transabdominal imaging. The integration of photoacoustic (PA) imaging with clinical US imaging has displayed promising outcomes in clinical research. Nonetheless, its application has been constrained due to size limitations, restricting it to spatially confined locations such as vaginal or rectal canals. Aim This study presents the development of a video-rate (20 Hz) endocavity PA/harmonic US imaging (EPAUSI) system. Approach The approach incorporates a commercially available endocavity US probe with a miniaturized laser delivery unit, comprised of a single large-core fiber and a line beamshaping engineered diffuser. The system facilitates real-time image display and subsequent processing, including angular energy density correction and spectral unmixing, in offline mode. Results The spatial resolutions of the concurrently acquired PA and harmonic US images were measured at and in the radial direction, respectively, and 1.22 deg and 1.50 deg in the angular direction, respectively. Furthermore, the system demonstrated its capability in multispectral PA imaging by successfully distinguishing two clinical dyes in a tissue-mimicking phantom. Its rapid temporal resolution enabled the capture of kinetic dye perfusion into an ex vivo porcine ovary through the depth of porcine uterine tissue. EPAUSI proved its clinical viability by detecting pulsating hemodynamics in the male rat’s prostate in vivo and accurately classifying human blood vessels into arteries and veins based on measurements. Conclusions Our proposed EPAUSI system holds the potential to unveil previously overlooked indicators of vascular alterations in genital cancers or endometriosis, addressing pressing requirements in the fields of gynecology and urology.

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An Ultraviolet‐Transparent Ultrasound Transducer Enables High‐Resolution Label‐Free Photoacoustic Histopathology

Cited by 9 publications

References 53 publications

High-resolution label-free ultraviolet photoacoustic microscopy via an ultraviolet-transparent ultrasound transducer

High-resolution label-free ultraviolet photoacoustic microscopy via an ultraviolet-transparent ultrasound transducer

Multiparametric tumor analysis through photoacoustic tomography system using a hemispherical transducer array

Video-rate endocavity photoacoustic/harmonic ultrasound imaging with miniaturized light delivery

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