Ultrastructure of type-I salivary-gland acini in four species of ticks and the influence of hydration states on the type-I acini ofAmblyomma americanum

Needham, Glen R.; Rosell, Rosemarie C.; Greenwald, Lewis

doi:10.1007/bf01194085

Cited by 21 publications

(26 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In type I acini ( Fig. 2B ), immunoreactivity reveals a vertical pattern toward the basolateral surface, as found in a previous study 30 . The immunoreactive abluminal epithelial cells containing small nuclei in type II acini were located between granular cells ( Fig.…”

Section: Resultssupporting

confidence: 87%

See 1 more Smart Citation

Multiple functions of Na/K-ATPase in dopamine-induced salivation of the Blacklegged tick, Ixodes scapularis

Kim

Urban

Boyle

et al. 2016

Sci Rep

View full text Add to dashboard Cite

Control of salivary secretion in ticks involves autocrine dopamine activating two dopamine receptors: D1 and Invertebrate-specific D1-like dopamine receptors. In this study, we investigated Na/K-ATPase as an important component of the secretory process. Immunoreactivity for Na/K-ATPase revealed basal infolding of lamellate cells in type-I, abluminal interstitial (epithelial) cells in type-II, and labyrinth-like infolding structures opening towards the lumen in type-III acini. Ouabain (10 μmol l−1), a specific inhibitor of Na/K-ATPase, abolished dopamine-induced salivary secretion by suppressing fluid transport in type III acini. At 1 μmol l−1, ouabain, the secreted saliva was hyperosmotic. This suggests that ouabain also inhibits an ion resorptive function of Na/K-ATPase in the type I acini. Dopamine/ouabain were not involved in activation of protein secretion, while dopamine-induced saliva contained constitutively basal level of protein. We hypothesize that the dopamine-dependent primary saliva formation, mediated by Na/K-ATPase in type III and type II acini, is followed by a dopamine-independent resorptive function of Na/K-ATPase in type I acini located in the proximal end of the salivary duct.

show abstract

Section: Resultssupporting

confidence: 87%

“…Moreover, silencing of the Na/K-ATPase gene via RNA interference demonstrated the importance of Na/K-ATPase to blood feeding and oviposition 29 . An ultrastructural study of type I acini visualized rich Na/K-ATPase-like reactions by the use of a phosphatase cytochemical method 30 .…”

mentioning

confidence: 99%

Multiple functions of Na/K-ATPase in dopamine-induced salivation of the Blacklegged tick, Ixodes scapularis

Kim

Urban

Boyle

et al. 2016

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Ticks have a well-developed epicuticular system (Ecdysozoan heritage) in both hard and soft bodied ticks (Hackman, 1982). Ticks also have a specialized mechanism to prevent water loss in the type I agranular salivary gland acini that functions as salt secretory glands to absorb moisture from the air (Balashov, 1972;Needham and Teel, 1986;Needham et al 1990). This acinar system is found in argasids, ixodids Needham et al 1990), presumably in N. namaqua, but not in other Parasitiformes , supporting the monophy of ticks.…”

Section: Minimizing Water Loss In Terrestrial Arthropodsmentioning

confidence: 99%

“…Ticks also have a specialized mechanism to prevent water loss in the type I agranular salivary gland acini that functions as salt secretory glands to absorb moisture from the air (Balashov, 1972;Needham and Teel, 1986;Needham et al 1990). This acinar system is found in argasids, ixodids Needham et al 1990), presumably in N. namaqua, but not in other Parasitiformes , supporting the monophy of ticks. This is a lineage specific adaptation related to the evolution of hematophagy and parasitism that allow ticks to survive for prolonged periods between feeding events.…”

Section: Minimizing Water Loss In Terrestrial Arthropodsmentioning

confidence: 99%

Ancestral reconstruction of tick lineages

Mans

Castro

Pienaar

et al. 2016

Ticks and Tick-borne Diseases

101

View full text Add to dashboard Cite

“…Ixodid ticks can capture atmospheric water molecules using hygroscopic saliva in the microenvironment where the relative humidity is higher than critical equilibrium activity ( McMullen, Sauer & Burton, 1976 ; Rudolph & Knulle, 1974 ). Different types of salivary gland acini have been suggested to have different roles in the water balance in Ixodid ticks ( Coons et al, 1994 ; Kim, Šimo & Park, 2014 ; Krolak, Ownby & Sauer, 1982 ; Megaw & Beadle, 1979 ; Needham, Rosell & Greenwald, 1990 ).…”

Section: Introductionmentioning

confidence: 99%

Water absorption through salivary gland type I acini in the blacklegged tick,Ixodes scapularis

Kim

Maldonado-Ruiz

Zurek

et al. 2017

PeerJ

View full text Add to dashboard Cite

Tick salivary glands play critical roles in maintaining water balance for survival, as they eliminate excess water and ions during blood feeding on hosts. In the long duration of fasting in the off-host period, ticks secrete hygroscopic saliva into the mouth cavity to uptake atmospheric water vapor. Type I acini of tick salivary glands are speculated to be involved in secretion of hygroscopic saliva based on ultrastructure studies. However, we recently proposed that type I acini play a role in resorption of water/ions from the primary saliva produced by other salivary acini (i.e., types II and III) during the tick blood feeding phase. In this study, we tested the function of type I acini in unfed female Ixodes scapularis. The route of ingested water was tracked after forced feeding of water with fluorescent dye rhodamine123. We found that type-I acini of the salivary glands, but not type II and III, are responsible for water uptake. In addition, the ingestion of water through the midgut was also observed. Injection or feeding of ouabain, a Na/K-ATPase inhibitor, suppressed water absorption in type I acini. When I. scapularis was offered a droplet of water, ticks rarely imbibed water directly (5%), while some approached the water droplet to use the high humidity formed in the vicinity of the droplet (23%). We conclude that during both on- and off-host stages, type I acini in salivary glands of female Ixodes scapularis absorb water and ions.

show abstract

Ultrastructure of type-I salivary-gland acini in four species of ticks and the influence of hydration states on the type-I acini ofAmblyomma americanum

Cited by 21 publications

References 25 publications

Multiple functions of Na/K-ATPase in dopamine-induced salivation of the Blacklegged tick, Ixodes scapularis

Multiple functions of Na/K-ATPase in dopamine-induced salivation of the Blacklegged tick, Ixodes scapularis

Ancestral reconstruction of tick lineages

Water absorption through salivary gland type I acini in the blacklegged tick,Ixodes scapularis

Contact Info

Product

Resources

About