Ultrastructure of preimplantation genetic diagnosis-derived human blastocysts grown in a coculture system after vitrification

Escribá, Marı́a-José; Escobedo‐Lucea, Carmen; Mercader, Amparo; Santos, M. de los; Pellicer, António; Remohı́, J.

doi:10.1016/j.fertnstert.2006.01.033

Cited by 13 publications

(10 citation statements)

References 54 publications

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“…These findings confirm earlier results, which showed that warmed blastocysts that survived vitrification were not different from fresh blastocysts, in terms of quality, DNA and chromosome integrity, ultrastructure, and developmental competence [11,17,[22][23][24][25].…”

Section: Discussionsupporting

confidence: 91%

Survival, re-expansion and cell survival of human blastocysts following vitrification and warming using two vitrification systems

Lopes

Frederickx

Kerkhoven

et al. 2014

J Assist Reprod Genet

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Purpose This study evaluated and compared survival, re-expansion, and percentage of live cells of individual Days 5 and 6 human blastocysts that were vitrified and warmed with the Vit Kit Freeze/Thaw (Irvine Scientific, CA), or with two protocols using the Global Fast Freeze/Thaw Kits (LifeGlobal, Canada). Methods Frozen/thawed Day 2-3 or discarded embryos were cultured to blastocyst (culture day 5-6). Group 1 blastocysts were vitrified with the Vit Kit (n=29) and High Security Vitrification (HSV) devices. Group 2 (n=47) and Group 3 (n=48) blastocysts were cryopreserved with the Global Fast Freeze Kit and 0.25 ml straws, using a direct plunge or a −100°C holding step, respectively. Group 4 (Controls, n= 30) were not vitrified. Blastocysts were subsequently cultured for 24 h, assessed for survival and expansion, and then stained individually with propidium iodide and Hoechst. Live and total cell number was assessed with ImageJ (NIH), and the percentage of live cells calculated for each blastocyst. Results The percentage of live cells was not different between vitrified and control (non-vitrified) blastocysts, thus vitrification did not affect cell survival. Survival (following thawing and after 24 h culture), re-expansion, and percentage of live cells were not different for blastocysts vitrified and warmed between the two vitrification/warming kits, or between the two protocols for the Global Fast Freeze/Thaw Kits. Conclusions Blastocyst vitrification can be achieved with equal success using simplified protocols and cheaper and easy to load freezing straws, providing simultaneously increased safety, and efficiency with lower cost, when compared with vitrification using specialized embryo vitrification devices.

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Section: Discussionsupporting

confidence: 91%

Survival, re-expansion and cell survival of human blastocysts following vitrification and warming using two vitrification systems

Lopes

Frederickx

Kerkhoven

et al. 2014

J Assist Reprod Genet

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show abstract

“…During in vitro culture (C to E, Â40), some blastomeres cleaved (C), compacted (D), and cavitated (E). blastomere could be caused by the dynamic expression of its osmotic response (15)(16)(17)(18)(19)(20) or the pressure of both the VS and carrier on the surface of the sample, as discussed previously. All zonae included in this study were of a similar thickness (18.2 AE 2.9 mm [mean AE SD] and 12.3-24.5mm [95CI]) within the normal range of 10-31 mm (21).…”

Section: Figurementioning

confidence: 93%

Vitrification of isolated human blastomeres

Escribá

Grau

Escrich

et al. 2010

Fertility and Sterility

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“…In the present work, blastocysts were cryopreserved by our earlier reported vitrification and warming protocol (34)(35)(36). Such protocol was based on a work reported elsewhere by Yokota and co-workers (14), with modifications.…”

Section: Blastocyst Vitrification and Warming Proceduresmentioning

confidence: 99%

“…When we followed this protocol, nearly 50% of warmed blastocysts survived vitrification, were of good quality, and showed an ultrastructural feature comparable to noncryopreserved control embryos (36). Despite the reported limited survival rates, we retain this protocol using 0.25 mL-French straws as a safety device option for vitrification of human blastocysts.…”

mentioning

confidence: 97%

“…Elsewhere, we reported a vitrification procedure for human blastocysts by using an equimolar concentration of ethylene glycol and dimethyl sulfoxide as cryoprotectants and 0.25 mL-French straws as carriers (34)(35)(36). When we followed this protocol, nearly 50% of warmed blastocysts survived vitrification, were of good quality, and showed an ultrastructural feature comparable to noncryopreserved control embryos (36).…”

mentioning

confidence: 99%

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Vitrification of preimplantation genetically diagnosed human blastocysts and its contribution to the cumulative ongoing pregnancy rate per cycle by using a closed device

Escribá

Zulategui

Galán

et al. 2008

Fertility and Sterility

Self Cite

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Ultrastructure of preimplantation genetic diagnosis-derived human blastocysts grown in a coculture system after vitrification

Cited by 13 publications

References 54 publications

Survival, re-expansion and cell survival of human blastocysts following vitrification and warming using two vitrification systems

Survival, re-expansion and cell survival of human blastocysts following vitrification and warming using two vitrification systems

Vitrification of isolated human blastomeres

Vitrification of preimplantation genetically diagnosed human blastocysts and its contribution to the cumulative ongoing pregnancy rate per cycle by using a closed device

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