Vitrification of isolated human blastomeres

Comparison of Cryotip vs. Cryotop for mouse and human blastomere vitrification

“…In a previous publication (37), abnormal blastomeres were vitrified using the open Cryotop system, but only 2 of 21 (9%) blastomeres were alive after warming. However, when blastomeres were placed in a host/emptied ZP obtained from unfertilized oocytes, their survival rate increased to 75.7%.…”

“…Blastomere vitrification (BV) has been tested in different fish types: rainbow trout (26), salmon (27), goldfish (28), and zebra fish (29-31), among others (32), and also in bovines (33) and mice (34)(35)(36). There is one report in humans of 37 blastomeres from disaggregated abnormal embryos using the host's zona pellucida (ZP) to cover blastomeres via the Cryotop method (37).…”

Genome Banking for Vertebrates Wildlife Conservation

“…Such stem cells can also be derived from embryos generated by nuclear transfer of freeze-dried cells (Ono et al, 2008). Embryonic stem cells can also be derived from isolated blastomeres, and blastomers can also be cryopreserved individually by inserting them into emptied zona pellucida and then vitrifying them (Escriba et al, 2010). If embryonic stem cells are not available, somatic cells can be induced to become embryonic stem cells-like (Takahashi & Yamanaka, 2006), also known as induced pluripotent stem cells or iPS cells (for recent review see: Cox & Rizzino, 2010).…”

Mammalian reproduction out of cryopreserved cells and tissues: current state of the art and future options

“…These cells can be cryopreserved (Thomson et al , 1998; Toyooka et al , 2003) or vitrified (Reubinoff et al , 2001). Embryonic stem cells can also be derived from isolated blastomeres, and blastomeres can also be cryopreserved individually by inserting them into an emptied zona pellucida and then vitrifying them (Escribá et al , 2010).…”