Vitrification of preimplantation genetically diagnosed human blastocysts and its contribution to the cumulative ongoing pregnancy rate per cycle by using a closed device

Escribá, Marı́a-José; Zulategui, J.; Galán, A.; Mercader, Amparo; Remohı́, José; Santos, M. de los

doi:10.1016/j.fertnstert.2007.04.035

Cited by 26 publications

(18 citation statements)

References 52 publications

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“…The long period required for hybridization (up to 72 h) has limited the widespread use of this technique, as it is necessary to freeze the biopsied embryos, and the survival rate of the thawed embryos has been relatively poor (approximately 50% did not survive the thawing process) (Hu et al, 2004;Escribá et al, 2008). The development of a highly efficient cryopreservation technique is urgently needed to reduce fears concerning the impact of cryopreservation on embryo viability.…”

Section: Slippage Of Microsatellitesmentioning

confidence: 99%

Whole genome amplification in preimplantation genetic diagnosis

Zheng

Wang

et al. 2011

J. Zhejiang Univ. Sci. B

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Preimplantation genetic diagnosis (PGD) refers to a procedure for genetically analyzing embryos prior to implantation, improving the chance of conception for patients at high risk of transmitting specific inherited disorders. This method has been widely used for a large number of genetic disorders since the first successful application in the early 1990s. Polymerase chain reaction (PCR) and fluorescent in situ hybridization (FISH) are the two main methods in PGD, but there are some inevitable shortcomings limiting the scope of genetic diagnosis. Fortunately, different whole genome amplification (WGA) techniques have been developed to overcome these problems. Sufficient DNA can be amplified and multiple tasks which need abundant DNA can be performed. Moreover, WGA products can be analyzed as a template for multi-loci and multi-gene during the subsequent DNA analysis. In this review, we will focus on the currently available WGA techniques and their applications, as well as the new technical trends from WGA products.

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Section: Slippage Of Microsatellitesmentioning

confidence: 99%

Whole genome amplification in preimplantation genetic diagnosis

Zheng

Wang

et al. 2011

J. Zhejiang Univ. Sci. B

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“…Fortunately, different data in the literature report that blastocyst vitrification on day-5 or 6 is a successful method for cryopreservation with surprising higher survival rates than cleavage embryos in PGD programs [48,49].…”

Section: Blastocyst Biopsymentioning

confidence: 99%

Preimplantation genetic diagnosis and the biopsy technique: Important considerations

Greco¹,

Fabozzi²,

Ruberti³

et al. 2013

ARSci

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Preimplantation genetic diagnosis allows to test the genetic status of embryos prior to implantation. In order to obtain genetic material, on which carry out a genetic diagnosis, a procedure named embryo biopsy is required. In the last two decades, embryo biopsy at the cleavage stage has been the mostly performed procedure. However, recently, alternative methods allowing the retrieval of a larger number of cells (blastocyst stage biopsy), or representing a valid alternative to overcome ethical issues (polar body biopsy) have obtained increasing consensus. This article reviews different methods of embryo biopsy and points out their positive and negative aspects.

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“…It yields more biopsied cells (10-30 cells) and avoids removing an embryonic cell. Frozen trophectoderm-biopsied embryos are thawed during the next cycle for embryo transfer (de Boer et al 2004 ;Kokkali et al 2005 ;McArthur et al 2005 ;Kuwayama 2007 ;Escribá et al 2008 ;Schlenker et al 2009 ) .…”

Section: Obtaining Cells For Preimplantation Genetic Diagnosismentioning

confidence: 99%