Mesenchymal Stem Cells: Possibilities of New Treatment Options

Tokcaer-Keskin, Zeynep; Koçak, Hande; Gürsel, İhsan; Akçalı, Kamil Can

doi:10.1007/978-1-61779-630-2_6

Cited by 3 publications

(4 citation statements)

References 459 publications

(575 reference statements)

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“…MSCs have a high potential for regenerative medicine and tissue engineering not only due to their intrinsic self-renewal capacity and ability to differentiate functional cell types in specific tissues but also due to their homing capacity and nonimmunogenic features [ 1 , 2 , 7 ]. Since MSCs are important tools for cell-based therapies [ 25 – 27 ] and nanoparticles are promising labeling tools in noninvasive cell tracking [ 28 ], complete assessment of CPNs on stem cells should be performed.…”

Section: Discussionmentioning

confidence: 99%

“…Stem cells are the new hope for the century since they have the potential to differentiate into several types of cells and tissues. Applications involving the use of stem cells in humans that might have been considered “science fiction” fewer than 20 years ago are now being utilized with a great success rate [ 1 , 2 ]. Described by the pioneering studies of Friedenstein et al [ 3 ] in 1970, mesenchymal stem cells (MSCs) are multipotent cells, capable of self-renewal and differentiating into multiple lineages, such as osteocytes, adipocytes, chondrocytes, myoblasts, and cardiomyocytes [ 4 – 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…Described by the pioneering studies of Friedenstein et al [ 3 ] in 1970, mesenchymal stem cells (MSCs) are multipotent cells, capable of self-renewal and differentiating into multiple lineages, such as osteocytes, adipocytes, chondrocytes, myoblasts, and cardiomyocytes [ 4 – 6 ]. MSCs have a high potential for regenerative medicine and tissue engineering not only due to their intrinsic self-renewal capacity and ability to differentiate functional cell types in specific tissues but also due to their homing capacity and nonimmunogenic features [ 2 , 7 ]. Therefore use of MSCs may provide new strategies for many debilitating diseases including neurodegenerative and cardiovascular diseases, diabetes, and cancer [ 5 , 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

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Nanoparticle Labeling of Bone Marrow-Derived Rat Mesenchymal Stem Cells: Their Use in Differentiation and Tracking

Akhan

Tuncel

Akçalı

2015

BioMed Research International

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Mesenchymal stem cells (MSCs) are promising candidates for cellular therapies due to their ability to migrate to damaged tissue without inducing immune reaction. Many techniques have been developed to trace MSCs and their differentiation efficacy; however, all of these methods have limitations. Conjugated polymer based water-dispersible nanoparticles (CPN) represent a new class of probes because they offer high brightness, improved photostability, high fluorescent quantum yield, and noncytotoxicity comparing to conventional dyes and quantum dots. We aimed to use this tool for tracing MSCs' fate in vitro and in vivo. MSC marker expression, survival, and differentiation capacity were assessed upon CPN treatment. Our results showed that after CPN labeling, MSC markers did not change and significant number of cells were found to be viable as revealed by MTT. Fluorescent signals were retained for 3 weeks after they were differentiated into osteocytes, adipocytes, and chondrocytes in vitro. We also showed that the labeled MSCs migrated to the site of injury and retained their labels in an in vivo liver regeneration model. The utilization of nanoparticle could be a promising tool for the tracking of MSCs in vivo and in vitro and therefore can be a useful tool to understand differentiation and homing mechanisms of MSCs.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Nanoparticle Labeling of Bone Marrow-Derived Rat Mesenchymal Stem Cells: Their Use in Differentiation and Tracking

Akhan

Tuncel

Akçalı

2015

BioMed Research International

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“…[16][17][18] Inconsistent results were obtained by various authors during Isolation and defining the characteristic of MSC, as because isolation of MSC varied dramatically due to their species difference and adopting methodologies for expansion and plating of cells. 19,20 However bone marrow stem cells can be isolated by Ficoll gradient centrifugation, plastic adherence.…”

Section: Discussionmentioning

confidence: 99%

Culture, Characterization and Differentiation Potential of Rat Bone Marrow derived Mesenchymal Stem Cells

Tamilmahan¹

2016

JSRT

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Abbreviations: rBM-MSC, rat bone marrow derived mesenchymal stem cells; CFU-F, colony forming unit-fibroblast; DPBS, dulbecco's phosphate buffer saline; DMEM, dulbecco's modified eagles medium; RT PCR, reverse transcription polymerase chain reaction; RNA, ribonucleic acid IntroductionBone marrow hosts variety of tissues including hematopoietic lineage cells and mesenchymal stem cells (MSCs). The hematopoietic cells are the major source of the blood cells in the adult body which are regulated within the microenvironment of the stromal cells of the bone marrow.1,2 MSCs are multipotent cells and can be differentiate into various cell lineage depend upon their environment and culture condition in which they are kept. Rat bone marrow derived stem cells (rBM-MSCs) have long term self renewing and capabilities of pluripotency including osteoblast, adipocytes, chondrocytes, tenocytes, muscle cells and neurogenic cells, which make them ideal source of stem cells for regeneration of injured tissue. [3][4][5] Bone marrow derived mesenchymal stem cells have been isolated from different species. Report says that each species having different culturing and growing capacity. 6 For example human bone marrow derived stem cells are easy to harvest and maintain in culture condition where as rat MSCs is more difficult compared to other species. 7,8Technical difficulties in isolation limited the number of animal experiment and its required animal transplantation model for pre clinical studies.9 Density gradient centrifugation, 10 plastic adherence 11 and immunomagnetic selection 12,13 like various methods are available for isolation of MSCs from bone marrow. No appropriate methods are available for selection of suitable cells. Each method has their own pros and cons. Therefore this study combined density gradient centrifugation and plastic adherence for easy and reliable method for selection of suitable cells. Materials and methods Collection of bone marrowFour male wistar rats aged 8-14weeks old were prepared for collection of bone marrow. All procedure was undertaken after getting prior approval from Institutional Animal Ethics Committee (IAEC). Briefly after euthanasia, animals back side were cleanly shaved and prepared aseptically. The skin incision was made on the lateral aspect of thigh and both femur and tibia from one rat were taken after stripping out adherent muscles of the knee end. The collected bone was taken to laminar hood and placed in Petri dish containing DMEM (Dulbecco's Modified Eagles Medium) media with antibiotics. Both the metaphyseal region of femur was cut with scissors and needle was inserted into the bone to aspirate the content by flushing with 1ml of complete media. The cell suspension was centrifuged at 980rpm for 5min to concentrate the cells. The cell pellets were resuspended with 5ml of complete DMEM medium then layered over HISTOPAQUE -1077(Sigma) and centrifuged at 2500rpm for 30minutes. Mononuclear cells were collected from the interface by gradient centrifugation and washed with Ca+ and Mg+ free ...

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Cardiogenic differentiation of murine bone marrow-derived mesenchymal stem cells by 5-azacytidine: A follow-up In vitro Study

Eldin

Ibrahim

Mousa

et al. 2019

J Microsc Ultrastruct

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Background:Cell-based therapy is a promising tool in the management of myocardial infarction.Aim of the Work:The aim of this study is to examine the in vitro potential differentiation of murine bone marrow (BM)-derived stem cells into cardiomyocytes using 5-azacytidine after 1, 3, and 5 weeks and follow it up after 8 weeks.Materials and Methods:BM-derived mesenchymal stem cells (MSCs) were extracted from the bones of adult albino rats. MSCs were induced with 10 μM 5-azacytidine for 24 h. The cells were examined after 1, 3, 5, and 8 weeks. Cell characterization with immunocytochemistry for detection of CD105, desmin, and T-troponin and transmission electron microscopy was performed.Results:The 5-azacytidine-induced MSCs showed light and electron microscopic histological characteristics resembling cardiomyocytes and progressively expressed the cardiac muscle-specific markers over the 1st, 3rd, and 5th weeks, yet by the 8th week, these parameters were significantly downregulated.Conclusion:Prolonged survival of 5-azacytidine-induced MSCs in culture beyond the 8th week resulted in loss of the newly acquired cardiomyocyte characteristics. It is not recommended to prolong the maintenance of 5-azacytidine-induced MSCs in culture on the hope of increasing its cardiogenic potentiality beyond 5 weeks.

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Mesenchymal Stem Cells: Possibilities of New Treatment Options

Cited by 3 publications

References 459 publications

Nanoparticle Labeling of Bone Marrow-Derived Rat Mesenchymal Stem Cells: Their Use in Differentiation and Tracking

Nanoparticle Labeling of Bone Marrow-Derived Rat Mesenchymal Stem Cells: Their Use in Differentiation and Tracking

Culture, Characterization and Differentiation Potential of Rat Bone Marrow derived Mesenchymal Stem Cells

Cardiogenic differentiation of murine bone marrow-derived mesenchymal stem cells by 5-azacytidine: A follow-up In vitro Study

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