Ultrastructural observations on the phagocytic behavior of winter flounder Pleuronectes americanus peritoneal neutrophils and macrophages in vivo

Je, Bodammer; Ra, Robohm

doi:10.3354/dao025197

Cited by 11 publications

(9 citation statements)

References 29 publications

(45 reference statements)

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“…EGCs were readily identifiable by large, homogeneous electron-opaque granules, and neutrophils were differentiated from morphologically similar macrophages by the presence of characteristic striated granules. All cell types were morphologically consistent with previous reports (Bodammer 1986, Bodammer & Robohm 1996. Mycobacteria were rarely observed within nonaggregated macrophages.…”

supporting

confidence: 80%

Ultrastructure of Mycobacterium marinum granuloma in striped bass Morone saxatilis

Gauthier

Vogelbein²,

Ottinger³

2004

Dis. Aquat. Org.

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An emerging epizootic of mycobacteriosis currently threatens striped bass Morone saxatilis populations in Chesapeake Bay, USA. Several species of mycobacteria, including Mycobacterium marinum, species resembling M. avium, M. gordonae, M. peregrinum, M. scrofulaceum and M. terrae, and the new species M. shottsii have been isolated from diseased and healthy bass. In this study, we describe the ultrastructure of developing M. marinum granulomas in experimentally infected bass over a period of 45 wk. The primary host response to injected mycobacteria was formation of large macrophage aggregations containing phagocytosed bacilli. M. marinum were always contained within phagosomes. Close association of lysosomes with mycobacterial phagosomes, as well as the presence of electron-opaque material within phagosomes, suggested phagolysosomal fusion. Development of granulomas involved epithelioid transformation of macrophages, followed by appearance of central necrosis. Desmosomes were present between mature epithelioid cells. The necrotic core region of M. marinum granulomas was separated from overlying epithelioid cells by several layers of flattened, electron-opaque spindle-shaped cells. These cells appeared to be formed by compression of epithelioid cells and, aside from a flattened nucleus, did not possess recognizable organelles. Following the development of well-defined, paucibacillary granulomas, secondary disease was observed. Recrudescence was marked by bacterial replication followed by disruption of granuloma architecture, including loss of epithelioid and spindle cell layers. In advanced recrudescent lesions, normal tissue was replaced by macrophages, fibroblasts, and other inflammatory leukocytes. Large numbers of mycobacteria were observed, both intracellular and suspended in cellular debris. KEY WORDS: Mycobacteriosis · Striped bass · Morone saxatilis · Mycobacterium marinum · Granuloma · Ultrastructure Resale or republication not permitted without written consent of the publisherDis Aquat Org 62: [121][122][123][124][125][126][127][128][129][130][131][132] 2004 body granulomas are composed of a solid mass of epithelioid cells and giant cells, with the eliciting agent often visible in the center. The other major granuloma type, the immune granuloma, is formed in response to insoluble particles that stimulate a cell-mediated immune response, such as mycobacteria. Cytokines produced by T cells, such as IL-12 and interferon-γ, play an important role in formation and maintenance of immune granulomas (Cooper et al. 1993, Ehlers et al. 2000. A prominent feature of immune granulomas produced by mycobacteria such as Mycobacterium tuberculosis is caseous necrosis in the lesion core, which produces a highly acidic, anoxic environment that may serve to degrade otherwise refractory bacilli.The structure and development of mycobacterial granulomas in mammals, especially those produced in response to Mycobacterium tuberculosis and BCG (attenuated M. bovis), have been described (Papadimitriou & Spector 1972, Ad...

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supporting

confidence: 80%

Ultrastructure of Mycobacterium marinum granuloma in striped bass Morone saxatilis

Gauthier

Vogelbein²,

Ottinger³

2004

Dis. Aquat. Org.

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“…Despite the low temperature used in the in vitro study and the use of saline as a medium, it was possible to verify phagocytosis by some rare MØ after only 30 min of incubation of the 48 h implanted coverslips (this was the only time span that observations were made every 30 min up to the full 4 h period of incubation). This period is similar to data for fishes of more elevated temperatures (Honda et al, 1986;Bodammer & Robohm, 1996;Sørensen et al, 1997).…”

Section: Discussionsupporting

confidence: 87%

Phagocytosis and giant cell formation at 0°C by macrophage (MØ) of Notothenia coriiceps

SILVA¹

2002

Journal of Fish Biology

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“…This period is similar to data for fishes of more elevated temperatures (Honda et al, 1986;Bodammer & Robohm, 1996;Sørensen et al, 1997).…”

Section: Discussionsupporting

confidence: 87%

“…The macrophage (MØ) is the main phagocytic cell in fishes, being important in immunity to a variety of bacterial diseases and is also cytotoxic against some parasite larvae (Blazer, 1991;Zelikoff et al, 1991;Bodammer & Robohm, 1996;Zapata et al, 1996;Dalmo et al, 1997). The capacity of MØ to firmly adhere to glass surfaces and their prolonged survival in culture medium are two characteristics that can be used to differentiate and separate them from granulocytes and lymphocytes (Zapata et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Phagocytosis and giant cell formation at 0° C by macrophage (MØ) of Notothenia coriiceps

Silva

Staines

Hernandez‐Blazquez³

et al. 2002

Journal of Fish Biology

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Macrophages adhered to round glass coverslips that had been implanted into the abdominal cavity of Notothenia coriiceps, collected from Admiralty Bay, King George Island, and removed and incubated in vitro for 4 h at 0° C at various intervals. The macrophages phagocytosed yeast Saccharomyces cerevisiae cells suspended in saline. Bi‐nuclear macrophages were first observed after 6 h. The formation of giant cells and their phagocytic activity was observed only in fish which had been injected with 1 ml of Bacillus of Calmette and Guerin (BCG)‐suspension 24 h before coverslip implantation and the coverslips removed 15 days after the implantation. Phagocytosis and the formation of giant cells in an Antarctic fish at 0° C is described for the first time.

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Ultrastructural observations on the phagocytic behavior of winter flounder Pleuronectes americanus peritoneal neutrophils and macrophages in vivo

Cited by 11 publications

References 29 publications

Ultrastructure of Mycobacterium marinum granuloma in striped bass Morone saxatilis

Ultrastructure of Mycobacterium marinum granuloma in striped bass Morone saxatilis

Phagocytosis and giant cell formation at 0°C by macrophage (MØ) of Notothenia coriiceps

Phagocytosis and giant cell formation at 0° C by macrophage (MØ) of Notothenia coriiceps

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