Phagocytosis and giant cell formation at 0°C by macrophage (MØ) of Notothenia coriiceps

SILVA, JODIR PEREIRA DA

doi:10.1006/jfbi.2001.1858

Cited by 11 publications

(12 citation statements)

References 27 publications

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Section: Methodsmentioning

confidence: 99%

“…The liquid containing phagocytic cells (macrophages) was aspirated using a Pasteur pipette and centrifuged at 251.5 g for 5 min; the supernatant was discarded. Aliquots of the sediments were examined between a slide and coverslip, under phase contrast microscopy (400·) to determine the phagocytic capacity (PC = number of phagocytosing macrophages ⁄ 100 macrophages) of the macrophages as described by Silva et al (2002Silva et al ( , 2005 Liver and muscle samples (taken from the dorsal superior aspect, 2.0 cm from the left operculum) were collected from the fish. These samples were kept at )10°C and sent for analysis of ether-extracts (according to AOAC -Association of Official Analytical Chemists, 1984) at the Animal Nutrition Analytical Laboratory (Laborato´rio de Ana´lises em Nutric¸a˜o Animal -LANA) of UNESP ⁄ FCAVJ, Jaboticabal, SP.…”

Section: Methodsmentioning

confidence: 99%

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Effect of incorporating probiotics into the diet of matrinxã (Brycon amazonicus) breeders

Dias

Leonardo

Tachibana³

et al. 2011

Journal of Applied Ichthyology

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Summary The aim of the present study was to evaluate the influence of probiotics prepared from Bacillus subtilis (strain C‐3102‐Calsporin®; Calpis, Tokyo, Japan) as a dietary supplement on several reproductive aspects of female ‘matrinxã’(Brycon amazonicus), including hematological parameters, immunological characteristics and total lipid concentrations in muscle and liver tissue. The broodstock were kept in two ponds of 600 m2, at a density of 50 fish per pond, from March to November 2008. The fish were divided into two treatment groups: untreated control fish (T1), and fish fed with 10 g of probiotics per kg of ration (T2). Fish were fed a diet containing 32% crude protein (CP), 7% crude fat (CF), and 6.5% ether extract (EE) twice daily at a rate of 1% of the total biomass per day during the coldest months (May through August) and 3% during the warm season (March, April and September through November). During the reproductive period, 20 individuals from each treatment group were selected for the experiments; two doses of crude carp pituitary extract were given to females in 10‐h intervals, and one dose was given to the males at the same time the females were given the second dose. A total of 20 fish were used for the hematological analyses. After anesthetization, blood samples were withdrawn by caudal puncture to determine the total number of cells, differential and total leukocyte counts, thrombocyte count, hematocrit, hemoglobin rate and plasma cortisol and glucose levels. The hematimetric index mean corpuscular volume (VCM) and mean corpuscular hemoglobin concentration (MHCM) were calculated. Ten other ‘matrinxãs’ from the same groups were used to assess macrophage phagocytic activity and lipid levels. The results showed that females fed a probiotics‐supplemented diet exhibited an increase in numbers of oocytes and, consequently, had higher rates of fertilization and hatching of larvae. Probiotic‐treated fish also exhibited a significant increase in the phagocytic activity of macrophages, indicating an improvement in the immune system of breeders. Hematologic parameters were different in comparison to the time‐zero group, and plasma cortisol and glucose levels were higher in the females. The total lipids levels in muscle and liver tissue were lower in B. amazonicus that received feed supplemented with probiotics.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Effect of incorporating probiotics into the diet of matrinxã (Brycon amazonicus) breeders

Dias

Leonardo

Tachibana³

et al. 2011

Journal of Applied Ichthyology

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show abstract

“…After centrifugation, the supernatant was discarded and the pellet was resuspended in the remaining liquid and placed on a slide with a coverslip; phagocytes were counted under a phase‐contrast microscope. The number of active phagocytes and the total number of yeast cells inside phagocytic cells were determined, and the phagocytic capacity (PC) and phagocytic index (PI) were calculated according to the method described by Silva, Staines, Hernandez–Blazquez, Porto–Neto and Borges (2002), Silva, Porto–Neto, Borges and Jensch–Junior (2005).…”

Section: Methodsmentioning

confidence: 99%

Haematologic and immunologic parameters of bullfrogs,Lithobates catesbeianus, fed probiotics

Dias

Stéfani

Ferreira³

et al. 2009

Aquaculture Research

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The e¡ects of two probiotics (P 1^L actobacillus acidophilus, Bi¢dobacterium bi¢dum and Enterococcus faecium and P 2^B acillus subtilis) supplemented to commercial feed (40% crude protein) on the haematological and immunological parameters of the bullfrog Lithobates catesbeianus were studied. Two doses of each probiotic (5 and 10 g kg À 1 of food) were added to the diets and fed to frogs, totalling ¢ve treatments over112 days. Haematological analyses consisted of total and di¡erential leucocyte counts, erythrocyte and thrombocyte counts, haematocrit, haemoglobin levels and RBC indices (mean corpuscular volume, mean corpuscular haemoglobin^and mean corpuscular haemoglobin concentration) and the immunological parameters included phagocytic capacity and phagocytic index of peritoneal phagocytes. The results showed that the probiotics did not signi¢cantly in£uence any of the haematological parameters measured. However, immunological assays showed that the probiotics had an immunostimulating e¡ect. The greatest e¡ects were seen with probiotic P 1 fed at a dose of 10 g kg À 1 of diet and probiotic P 2 fed at 5 g kg À 1 of diet. Figure 2 Photomicrograph of abdominal lavage of bullfrogs (Lithobates catesbeianus) showing phagocytic cells with yeast in their interior (a^six yeasts, b^no yeast). Phase contrast microscope, Â 400 magni¢cation.

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“…Experiments were carried out in fibreglass tanks (1,000 l) filled with running sea water (1±1.0°C) in a temperature-controlled room at 0±1.0°C at the Biology Laboratories of the Brazilian Antarctic Station, "Comandante Ferraz", in which the recording of biometric data and identification of the fish took place (Silva et al 1998a(Silva et al , 1999. Following anaesthesia with benzocaine (50 ppm; Silva et al 2002), two square (2.0×2.0 cm) fullthickness excision wounds, removing scales, epidermis, dermis, hypoderm and most of the perimysium, were inflicted on both sides of the dorsal-lateral anterior region in each of the 21 fishes. Three fish were used as normal skin controls.…”

Section: Fish Maintenance and Wound Inductionmentioning

confidence: 99%

Microscopical study of experimental wound healing in Notothenia coriiceps (Cabeçuda) at 0°C

et al. 2005

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Notothenia coriiceps (Cabeçuda) is an Antarctic benthic fish frequently found with lesions in the tegument caused by seal predation. We have investigated epidermal repair in these animals by means of a microscopic study of experimental wound healing at 0 degrees C. At 24--48 h after wound induction, mucous exudate and necrotic lining cells covered the wound. At 7--14 days, an epidermal "tongue" could be discerned, folded at the tip, with intercellular oedema between the tip and the wound border. After 23--30 days, the wound was completely closed and the migrating epidermis, with intercellular oedema, was reduced. By 45--90 days, melanocytes progressively increased in the epidermis but no scales were formed. The inflammatory infiltrate was mainly composed of neutrophils after 7 days, at which time they were mostly replaced by macrophages; lymphocytes and plasma cells were also present. The border epidermis slid towards the centre, folding at the tip and finally fusing to form a diaphragm. The cells of the epidermis began to multiply only after complete closure of the wound. The lack of scale formation on induced and naturally found wounds, even after 90 days, suggests that different mechanisms in wound repair occur at 0 degrees C from those in fish from temperate and tropical environment. This is the first report of successful wound repair at polar temperatures, indicating the adaptation of N. coriiceps to the Antarctic environment.

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Phagocytosis and giant cell formation at 0°C by macrophage (MØ) of Notothenia coriiceps

Cited by 11 publications

References 27 publications

Effect of incorporating probiotics into the diet of matrinxã (Brycon amazonicus) breeders

Effect of incorporating probiotics into the diet of matrinxã (Brycon amazonicus) breeders

Haematologic and immunologic parameters of bullfrogs,Lithobates catesbeianus, fed probiotics

Microscopical study of experimental wound healing in Notothenia coriiceps (Cabeçuda) at 0°C

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