Phagocytosis and giant cell formation at 0° C by macrophage (MØ) of <i>Notothenia coriiceps</i>

Silva, J. R. M. C.; Staines, N. A.; Hernandez‐Blazquez, Francisco Javier; Porto-Neto, Laércio R.; Borges, João Carlos Shimada

doi:10.1111/j.1095-8649.2002.tb00294.x

Cited by 10 publications

(5 citation statements)

References 40 publications

(61 reference statements)

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“…Previous researches assessing in vivo phagocytosis in fish described methods using phase contrast microscope (Silva et al, 2002(Silva et al, , 2005Dias et al, 2011;Nunes et al, 2014;Farias et al, 2016) in order to facilitate the observation of the phagocytized yeasts inside of coelomic phagocyte. In the present study, the S. cerevisiae labeling enhanced the traceability of phagocytosis by making the phagocytized yeasts possible to be observed and counted under conventional light microscopy.…”

Section: Discussionmentioning

confidence: 99%

In vivo phagocytosis and hematology in Astyanax altiparanae, a potential model for surrogate technology

et al. 2020

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Abstract Although the potential of surrogate propagation technology for aquaculture and conservation of Neotropical fish, the poor understanding of the host immune system may results in rejection and destruction of the donor material. Thus, it is necessary to study and to develop methods to evaluate the effects of immunosuppressive drugs employment and to evaluate the immunocompatibility between donor and receptor. Thus, the present study aimed to optimize a methodology to assess in vivo phagocytosis in Astyanax altiparanae using Saccharomyces cerevisiae and to evaluate their hematological response resultant from the inflammatory induction. To this, S. cerevisiae were labeled with Congo red and injected in the coelomic cavity of A. altiparanae at the concentration of 2.5 x 106 cells mL-1. A PBS solution and a non-injected group were kept as control. Fish blood was sampled and the phagocytic capacity and index were determined at 1, 2, 3 and 6 h post-injection (hpi). The yeast injection successfully stimulated phagocytosis, with the best result for phagocytosis assessment after 2 hpi. Moreover, it was achieved a high traceability of phagocytized and non-phagocytized yeast under optic microscopy analysis due to the Congo red labeling. The hematological profile was similar to usually observed in early infections, indicating lymphocyte migration to inflammatory site and increase in number of circulating phagocytes due to natural response to inflammatory stimulus. In conclusion, our method was efficient to assess in vivo phagocytosis in A. altiparanae and will be an important tool to evaluate the efficacy of immunosuppressive drugs in this species. Additionally, these results may serve as support for further studies in fish immunocompetence, both in laboratory and in field conditions.

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Section: Discussionmentioning

confidence: 99%

In vivo phagocytosis and hematology in Astyanax altiparanae, a potential model for surrogate technology

et al. 2020

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“…Both innate and adaptive immune components must work with each other efficiently to prevent body from any diseases, where innate immunity responds to invading pathogens by identifying line-encoded molecules of the germ. Toll-like receptors (TLRs) and phagocytosis are the essential components of innate immunity that shields the host from foreign invaders by identifying and ultimately killing phagocyte cells (Silva et al 2002). In contrast, adaptive immunity recognizes pathogens through molecules created by somatic mechanisms followed by humoral and cellular reactions via B-and T-lymphocytes (Uribe et al 2011).…”

Section: Fish Immune Systemmentioning

confidence: 99%

A review article on nanotechnology in aquaculture sustainability as a novel tool in fish disease control

et al. 2021

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In recent decades, aquaculture has played a significant role in fulfilling the vast demand for animal protein requirements and consequently in food security. However, environmental contamination and disease prevalence are considered essential challenges for the sector. In this regard, new approaches have been paved in technology to deal effectively with such challenges. Among these, nanotechnology-as a novel and innovative tool-has a broad spectrum of uses and a tremendous potential in aquaculture and seafood preservation. It can provide new technologies for management of drugs as liberation of vaccines and therefore hold the assurance for civilized protection of farmed fish against disease-causing pathogens. This article presents a review of nanotechnology and its applications in aquaculture. Additionally, it gives a brief idea about the fish disease and classical ways of controlling pathogens. On the other hand, this review sheds the light on nanotechnology as a potential novel tool which may possibly enhance the management and the control of disease prevalence. Therefore, the importance of this technology to promote sustainable aquaculture has also been highlighted. Focusing on the role of selenium nanoparticles as an efficient element is discussed also in this article.

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“…The fish immune system comprises two main components: the innate and adaptive immune systems [23]. Innate immunity is activated by pathogens and serves as the host's first line of defence against infections [24].…”

Section: Introductionmentioning

confidence: 99%

The Immune Response to the Myxozoan Parasite Myxobolus cerebralis in Salmonids: A Review on Whirling Disease

Akram,

El-Matbouli,

Saleh

2023

IJMS

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Salmonids are affected by the economically significant whirling disease (WD) caused by the myxozoan parasite Myxobolus cerebralis. In the past, it was endemic to Eurasia, but it has now spread to different regions of North America, Europe, New Zealand, and South Africa. Among salmonids, rainbow trout is considered the most highly susceptible host. Upon entering to the host’s body, the parasite invades the spine and cranium, resulting in whirling behaviour, a blackened tail, and destruction of cartilage. The disease is characterized by the infiltration of numerous inflammatory cells, primarily lymphocytes and macrophages, with the onset of fibrous tissue infiltration. Several efforts have been undertaken to investigate the role of various immune modulatory molecules and immune regulatory genes using advanced molecular methods including flow cytometry and transcriptional techniques. Investigation of the molecular and cellular responses, the role of STAT3 in Th17 cell differentiation, and the inhibitory actions of suppressors of cytokine signaling (SOCS) on interferons and interleukins, as well as the role of natural resistance-associated macrophage proteins (Nramp) in WD have significantly contributed to our understanding of the immune regulation mechanism in salmonids against M. cerebralis. This review thoroughly highlights previous research and discusses potential future directions for understanding the molecular immune response of salmonids and the possible development of prophylactic approaches against WD.

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Phagocytosis and giant cell formation at 0° C by macrophage (MØ) of Notothenia coriiceps

Cited by 10 publications

References 40 publications

In vivo phagocytosis and hematology in Astyanax altiparanae, a potential model for surrogate technology

In vivo phagocytosis and hematology in Astyanax altiparanae, a potential model for surrogate technology

A review article on nanotechnology in aquaculture sustainability as a novel tool in fish disease control

The Immune Response to the Myxozoan Parasite Myxobolus cerebralis in Salmonids: A Review on Whirling Disease

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